Transcript: Human XM_017014734.1

PREDICTED: Homo sapiens muscle associated receptor tyrosine kinase (MUSK), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MUSK (4593)
Length:
8737
CDS:
717..3092

Additional Resources:

NCBI RefSeq record:
XM_017014734.1
NBCI Gene record:
MUSK (4593)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148371 TACCCATTGTAGTACATGGT pXPR_003 AGG 422 18% 4 0.783 MUSK MUSK 77500
2 BRDN0001147656 GCAGTCTTGAGTCAATCACT pXPR_003 CGG 824 35% 8 0.7034 MUSK MUSK 77503
3 BRDN0001146773 CTTTGGAATATGCTGTCCCG pXPR_003 AGG 590 25% 5 0.2731 MUSK MUSK 77501
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017014734.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002167 GTCATTTACTACGTGCGAGAT pLKO.1 2913 CDS 100% 4.050 3.240 N MUSK n/a
2 TRCN0000356242 GACTGCCACATCTAGATTATA pLKO_005 1828 CDS 100% 15.000 10.500 N MUSK n/a
3 TRCN0000356283 ACATGCATAGCTACCAATAAG pLKO_005 1587 CDS 100% 13.200 9.240 N MUSK n/a
4 TRCN0000361449 ACATGCATAGCTACCAATAAG pLKO_005 1587 CDS 100% 13.200 9.240 N Musk n/a
5 TRCN0000195290 CAAGTGAAGATGAAACCTAAA pLKO.1 1062 CDS 100% 10.800 7.560 N MUSK n/a
6 TRCN0000002166 GCAGACTACTACAAAGCTAAT pLKO.1 2739 CDS 100% 10.800 7.560 N MUSK n/a
7 TRCN0000002165 CCAGGACTCTACACATGCATA pLKO.1 1575 CDS 100% 4.950 3.465 N MUSK n/a
8 TRCN0000002164 GTAAGTTTGTTCACCGAGATT pLKO.1 2638 CDS 100% 4.950 3.465 N MUSK n/a
9 TRCN0000356243 GGCCCATGAGGAGGTCATTTA pLKO_005 2900 CDS 100% 13.200 7.920 N MUSK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017014734.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06606 pDONR223 100% 98.9% 98.8% None 402G>A;1127_1150del n/a
2 ccsbBroad304_06606 pLX_304 0% 98.9% 98.8% V5 402G>A;1127_1150del n/a
3 TRCN0000465498 ACCCGCCCGAGAACTTTAAAAATA pLX_317 15.3% 98.9% 98.8% V5 402G>A;1127_1150del n/a
4 ccsbBroadEn_14704 pDONR223 0% 88.8% 88.8% None 375T>C;628_657del;948_949ins264 n/a
5 ccsbBroad304_14704 pLX_304 0% 88.8% 88.8% V5 375T>C;628_657del;948_949ins264 n/a
6 TRCN0000488630 CAGTCTGCATCGTGCCGCATACGT pLX_317 24% 40% 40% V5 (not translated due to prior stop codon) 1_1422del n/a
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