Construct: ORF TRCN0000465566

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007296.1_s317c1
Derived from:
ccsbBroadEn_06379
DNA Barcode:
TATGGTTATTATAGCAGACTCGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HLA-G (3135)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465566
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3135 HLA-G major histocompatibility co... NM_002127.5 99.1% 99.1% (many diffs)
2 human 3135 HLA-G major histocompatibility co... XM_024446420.1 99.1% 99.1% (many diffs)
3 human 3135 HLA-G major histocompatibility co... NM_001363567.1 97.6% 97.6% (many diffs)
4 human 3134 HLA-F major histocompatibility co... XM_011514564.1 83.5% 75.4% (many diffs)
5 human 3105 HLA-A major histocompatibility co... NM_002116.8 83.1% 75.8% (many diffs)
6 human 3134 HLA-F major histocompatibility co... NM_018950.2 82.8% 75% (many diffs)
7 human 3105 HLA-A major histocompatibility co... NM_001242758.1 82.5% 74.7% (many diffs)
8 human 3106 HLA-B major histocompatibility co... NM_005514.8 81.8% 74.5% (many diffs)
9 human 3107 HLA-C major histocompatibility co... NM_001243042.1 81.3% 73.4% (many diffs)
10 human 3107 HLA-C major histocompatibility co... NM_002117.6 81.2% 73.2% (many diffs)
11 human 3133 HLA-E major histocompatibility co... NM_005516.6 80.6% 70.3% (many diffs)
12 human 3133 HLA-E major histocompatibility co... XM_017010809.2 80.3% 70.1% (many diffs)
13 human 3134 HLA-F major histocompatibility co... XM_017010813.1 74% 67% (many diffs)
14 human 3135 HLA-G major histocompatibility co... XM_017010817.1 71.9% 71.5% (many diffs)
15 human 3135 HLA-G major histocompatibility co... XM_017010818.1 71.9% 71.5% (many diffs)
16 human 3133 HLA-E major histocompatibility co... XM_017010807.1 71.4% 57.9% (many diffs)
17 human 3133 HLA-E major histocompatibility co... XM_017010808.1 71.4% 57.9% (many diffs)
18 human 3134 HLA-F major histocompatibility co... XM_017010812.1 68.4% 61.9% (many diffs)
19 human 3134 HLA-F major histocompatibility co... XM_017010811.1 66.5% 60.1% (many diffs)
20 human 3134 HLA-F major histocompatibility co... XM_017010810.1 66.1% 59.8% (many diffs)
21 human 3134 HLA-F major histocompatibility co... NM_001098479.2 65.1% 58.8% (many diffs)
22 human 3134 HLA-F major histocompatibility co... NM_001098478.2 59.2% 52.1% (many diffs)
23 human 3134 HLA-F major histocompatibility co... XM_017010815.1 54.4% 50.1% (many diffs)
24 human 3136 HLA-H major histocompatibility co... NR_001434.4 52.8% (many diffs)
25 human 3134 HLA-F major histocompatibility co... XM_017010814.1 42.7% 39.3% (many diffs)
26 human 3134 HLA-F major histocompatibility co... XR_001743373.1 27.1% (many diffs)
27 human 3134 HLA-F major histocompatibility co... XR_001743374.1 26.1% (many diffs)
28 human 3134 HLA-F major histocompatibility co... XR_001743376.1 25.9% (many diffs)
29 human 3139 HLA-L major histocompatibility co... NR_027822.1 15.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1080
ORF length:
1014
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ggtcatggca ccccgaaccc tcttcctgct actctcgggg gccctgaccc
121tgaccgagac ctgggcgggc tcccactcca tgaggtattt cagcgccgcc gtgtcccggc
181ccagccgcgg ggagccccgc ttcatcgcca tgggctacgt ggacgacacg cagttcgtgc
241ggttcgacag cgactcggcg tgtccgagga tggagccgcg ggcgccgtgg gtggagcggg
301aggggccaga gtattgggaa gaggagacac ggaacaccaa ggcccacgca cagactgaca
361gaatgaacct gcagaccctg cgcggctact acaaccagag cgaggccagt tctcataccc
421tccagtggat gattggctgc gacctggggt ccgacggacg cctcctccgc gggtatgaac
481agtatgccta cgatggcaag gattacctcg ccctgaacga ggacctgcgc tcctggaccg
541cagcggacac tgcggctcag atctccaagc gcaagtgtga ggcggccaat gtggctgaac
601aaaggagagc ctacctggag ggcacgtgcg tggagtggct ccacagatac ctggagaacg
661ggaaggagat gctgcagcgc gcggaccccc ccaagacaca cgtgacccac caccctgtct
721ttgactatga ggccacccTG AGGTGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCATAC
781TGACCTGGCA GCGGGATGGG GAGGACCAGA CCCAGGACGT GGAGCTCGTG GAGACCAAGC
841CTGCAGGGGA TGGAACCTTC CAGAAGTGGG CAGCTGTGGT GGTGCCTTCT GGAGAGGAGC
901AGAGATACAC GTGCCATGTG CAGCATGAGG GGCTGCCGGA GCCCCTCATG CTGAGATGGA
961AGCAGTCTTC CCTGCCCACC ATCCCCATCA TGGGTATCGT TGCTGGTCTG GTTGTCCTTG
1021CAGCTGTAGT CACTGGAGCT GCGGTCGCTG CTGTGCTGTG GAGGAAGAAG AGCTCAGATT
1081GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1141GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1201TTTATATATC TTGTGGAAAG GACGATATGG TTATTATAGC AGACTCGACA CGCGTTAAGT
1261Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)