Construct: ORF TRCN0000465646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006541.1_s317c1
- Derived from:
- ccsbBroadEn_14524
- DNA Barcode:
- TTCTAAGGCGATGGCTCCTTCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465646
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4141 | MARS1 | methionyl-tRNA synthetase 1 | XR_001748704.2 | 63.7% | (many diffs) | |
| 2 | human | 10677 | AVIL | advillin | NM_006576.3 | 45.9% | 43.5% | (many diffs) |
| 3 | human | 10677 | AVIL | advillin | XM_017018710.2 | 45.9% | 43.5% | (many diffs) |
| 4 | human | 10677 | AVIL | advillin | XM_017018711.2 | 44.9% | 43.6% | (many diffs) |
| 5 | human | 10677 | AVIL | advillin | XM_017018712.2 | 44.9% | 43.6% | (many diffs) |
| 6 | human | 10677 | AVIL | advillin | XM_017018713.2 | 44.9% | 43.6% | (many diffs) |
| 7 | human | 10677 | AVIL | advillin | XM_024448800.1 | 44.9% | 43.6% | (many diffs) |
| 8 | human | 10677 | AVIL | advillin | XM_017018714.2 | 22.2% | 20.4% | (many diffs) |
| 9 | human | 10677 | AVIL | advillin | XM_017018715.2 | 21.1% | 19.8% | (many diffs) |
| 10 | mouse | 11567 | Avil | advillin | XM_006513102.2 | 42.8% | 40.3% | (many diffs) |
| 11 | mouse | 11567 | Avil | advillin | XM_006513097.3 | 41.8% | 39.4% | (many diffs) |
| 12 | mouse | 11567 | Avil | advillin | XM_006513098.3 | 41.8% | 39.4% | (many diffs) |
| 13 | mouse | 11567 | Avil | advillin | NM_009635.3 | 41.1% | 40.2% | (many diffs) |
| 14 | mouse | 11567 | Avil | advillin | XM_006513099.3 | 40.2% | 39.3% | (many diffs) |
| 15 | mouse | 11567 | Avil | advillin | XM_006513100.3 | 40.2% | 39.3% | (many diffs) |
| 16 | mouse | 11567 | Avil | advillin | XM_006513101.2 | 40.2% | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3054
- ORF length:
- 2988
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag actgttcgtg agtgatggcg tcccgggttg cttgccggtg ctggccgccg 121 ccgggagagc ccggggcaga gcagaggtgc tcatcagcac tgtaggcccg gaagattgtg 181 tggtcccgtt cctgacccgg cctaaggtcc ctgtcttgca gctggatagc ggcaactacc 241 tcttctccac tagtgcaatc tgccgatatt tttttttgtt atctggctgg gagcaagatg 301 acctcactaa ccagtggctg gaatgggaag cgacagagct gcagccagct ttgtctgctg 361 ccctgtacta tttagtggtc caaggcaaga agggggaaga tgttcttggt tcagtgcgga 421 gagccctgac tcacattgac cacagcttga gtcgtcagaa ctgtcctttc ctggctgggg 481 agacagaatc tctagccgac attgttttgt ggggagccct atacccatta ctgcaagatc 541 ccgcctacct ccctgaggag ctgagtgccc tgcacagctg gttccagaca ctgagtaccc 601 aggaaccatg tcagcgagct gcagagactg tactgaaaca gcaaggtgtc ctggctctcc 661 ggccttacct ccaaaagcag ccccagccca gccccgctga gggaagggct gtcaccaatg 721 agcctgagga ggaggagctg gctaccctat ctgaggagga gattgctatg gctgttactg 781 cttgggagaa gggcctagaa agtttgcccc cgctgcggcc ccagcagaat ccagtgttgc 841 ctgtggctgg agaaaggaat gtgctcatca ccagtgccct cccttacgtc aacaatgtcc 901 cccaccttgg gaacatcatt ggttgtgtgc tcagtgccga tgtctttgcc aggtactctc 961 gcctccgcca gtggaacacc ctctatctgt gtgggacaga tgagtatggt acagcaacag 1021 agaccaaggc tctggaggag ggactaaccc cccaggagat ctgcgacaag taccacatca 1081 tccatgctga catctaccgc tggtttaaca tttcgtttga tatttttggt cgcaccacca 1141 ctccacagca gaccaaaatc acccaggaca ttttccagca gttgctgaaa cgaggttttg 1201 tgctgcaaga tactgtggag caactgcgat gtgagcactg tgctcgcttc ctggctgacc 1261 gcttcgtgga gggcgtgtgt cccttctgtg gctatgagga ggctcggggt gaccagtgtg 1321 acaagtgtgg caagctcatc aatgctgtcg agcttaagaa aatggagctg gcgctggtgc 1381 ctgtgagcgc ccacggcaac ttctatgagg gggactgcta cgtcatcctc tcgacccgga 1441 gagtggccag tctcctatcc caggacatcc acttctggat cgggaaggac tcctcccagg 1501 atgagcaaag ctgcgcagcc atatatacca cacagctgga cgactacctg ggaggcagcc 1561 ctgtgcagca ccgagaggtc cagtaccatg agtcagacac tttccgtggc tacttcaagc 1621 agggcatcat ctacaagcag gggggtgtcg cctctgggat gaagcacgtg gagaccaata 1681 cctacgacgt gaagcggctg ctacatgtga aagggaaaag aaacatcagg gctaccgagg 1741 tggaaatgag ctgggacagt ttcaaccgag gtgatgtctt cttgctggac cttgggaaag 1801 tcatcatcca atggaatggc ccagagagca acagtgggga gcgcctgaag gctatgcttc 1861 tggcaaagga tattcgagac agggagcgag ggggccgtgc tgaaatagga gtgatcgagg 1921 gagacaagga ggcagccagc ccagagctga tgaaggtcct tcaggacacc cttggccgac 1981 gctccattat caagcctaca gtccctgatg agatcataga tcagaagcag aaatcaacta 2041 tcatgttgta tcatatctca gattcagctg ggcagctggc agtcacagag gtagcaacaa 2101 ggcctctggt ccaggactta ctgaaccatg atgactgcta catcctggac caaagtggaa 2161 ccaaaatcta cgtgtggaaa ggaaaaggag ccacaaaggc tgaaaaacag gcagccatgt 2221 ctaaagcgct gggcttcatc aagatgaaga gctaccccag cagcaccaat gtggagaccg 2281 tcaacgatgg tgctgagtcg gccatgttca agcagctgtt ccagaagtgg tcagtaaagg 2341 accagaccat gggcctgggg aaaacgttca gcattggtaa aattgctaaa gttttccagg 2401 ataaatttga tgtgactctg ctacacacca agccagaggt agctgcccag gaaagaatgg 2461 tcgatgatgg caacggaaaa gttgaggtct ggagaattga gaacctggag ctggtccctg 2521 tggagtatca atggtatggc ttcttttatg ggggagactg ttatctggtc ctctacacat 2581 acgaggtaaa tgggaagcca catcacatct tgtacatctg gcagggccgc cacgcctcac 2641 aggatgagct ggcagcctca gcataccagg cagtggaggt ggatcggcag tttgatgggg 2701 ctgctgtgca ggttcgagtc aggatgggaa cggagccacg ccacttcatg gccaTCTTCA 2761 AAGGGAAGCT AGTTATCTTT GAGGGTGGGA CTTCCAGGAA GGGAAATGCC GAGCCTGACC 2821 CTCCAGTAAG ACTCTTCCAA ATTCATGGAA ATGACAAATC TAACACCAAA GCAGTGGAAG 2881 TTCCAGCCTT TGCCTCCTCC CTAAACTCCA ATGATGTCTT TCTGCTGCGA ACTCAGGCAG 2941 AGCACTACCT GTGGTATGGC AAGGTAGGAT GGCTGGGCCC CGGGTCAGAC CAGCCCCTCG 3001 GAGCCCAGAC CTGCACTCCC CTCCTCCTGT CAGCAAGGTC AAAGGACCTA GAGTACCCAA 3061 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 3121 ATTCTACGTA GTAATGAACT AGCCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 3181 TATCTTGTGG AAAGGACGAT TCTAAGGCGA TGGCTCCTTC TAAACGCGTT AAGTCgacaa 3241 tcaacctctg gattacaaaa tttgtgaaag att