Construct: ORF TRCN0000465716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016884.1_s317c1
- Derived from:
- BRDN0000395120
- DNA Barcode:
- CCCGCTAGCACTCTAGGATCTGGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TP53BP1 (7158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | NM_005657.4 | 99.8% | .4% | (many diffs) |
2 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | NM_001141980.3 | 99.6% | .4% | (many diffs) |
3 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | NM_001141979.3 | 99.5% | .4% | (many diffs) |
4 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | XM_011521985.2 | 97.1% | .4% | (many diffs) |
5 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | XM_011521986.3 | 85.4% | .4% | (many diffs) |
6 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | XR_931899.2 | 62% | (many diffs) | |
7 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | XR_001751382.2 | 56.1% | (many diffs) | |
8 | human | 7158 | TP53BP1 | tumor protein p53 binding p... | NM_001355001.2 | 51.5% | .6% | 0_1ins2864;2397_2397delTinsAC;2403A>T |
9 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_006499662.3 | 83.4% | .4% | (many diffs) |
10 | mouse | 27223 | Trp53bp1 | transformation related prot... | NM_013735.4 | 83.2% | .4% | (many diffs) |
11 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_011239581.2 | 83.2% | .4% | (many diffs) |
12 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_006499661.3 | 83.2% | .4% | (many diffs) |
13 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_011239582.2 | 82% | .4% | (many diffs) |
14 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_011239583.2 | 81.3% | .4% | (many diffs) |
15 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_017318936.1 | 81.2% | .4% | (many diffs) |
16 | mouse | 27223 | Trp53bp1 | transformation related prot... | NM_001290830.1 | 80.9% | .4% | (many diffs) |
17 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_011239584.2 | 80.9% | .4% | (many diffs) |
18 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_006499666.3 | 74.4% | .1% | (many diffs) |
19 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_017318938.1 | 72.4% | .1% | (many diffs) |
20 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_006499667.3 | 67.4% | .1% | (many diffs) |
21 | mouse | 27223 | Trp53bp1 | transformation related prot... | XM_006499668.3 | 44.4% | .7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 126
- ORF length:
- 57
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaccctact ggaagcagtg agattcagat ttctctcagc aagatactcc 121 ttgcctgata attgaagatt ctcagcctga aagccaggtt ctagaggatg attctggttc 181 tcacttcagt atgctatctc gacaccttcc taatctccag acgcacaaag aaaatcctgt 241 gttggatgtt gtgtccaatc ctgaacaaac agctggagaa gaacgaggag acggtaatag 301 tgggttcaat gaacatttga aagaaaacaa ggttgcagac cctgtggatt cttctaactt 361 ggacacatgt ggttccatca gtcaggtcat tgagcagtta cctcagccaa acaggacaag 421 cagtgttctg ggaatgtcag tggaatctgc tcctgctgtg gaggaagaga agggagaaga 481 gttggaacag aaggagaaag agaaggaaga agatacttca ggcaatacta cacattccct 541 tggtgctgaa gatactgcct catcacagtt gggttttggg gttctggaac tctcccagag 601 ccaggatgtt gaggaaaata ctgtgccata tgaagtggac aaagagcagc tacaatcagt 661 aaccaccaac tctggttata ccaggctgtc tgatgtggat gctaatactg caattaagca 721 tgaagaacag tccaacgaag atatccccat agcagaacag tccagcaagg acatccctgt 781 gacagcacag cccagtaagg atgtacatgt tgtaaaagag caaaatccac cacctgcaag 841 gtcagaggac atgcctttta gccccaaagc atctgttgct gctatggaag caaaagaaca 901 gttgtctgca caagaactta tggaaagtgg actgcagatt cagaagtcac cagagcctga 961 ggttttgtca actcaggaag acttgtttga ccagagcaat aaaacagtat cttctgatgg 1021 ttgctctact ccttcaaggg aggaaggtgg gtgttctttg gcttccactc ctgccaccac 1081 tctgcatctc ctgcagctct ctggtcagag gtcccttgtt caggacagtc tttccacgaa 1141 ttcttcagat cttgttgctc cttctcctga tgctttccga tctactcctt ttatcgttcc 1201 tagcagtccc acagagcaag aagggagaca agataagcca atggacacgt cagtgttatc 1261 tgaagaagga ggagagcctt ttcagaagaa acttcaaagt ggtgaaccag tggagttaga 1321 aaacccccct ctcctgcctg agtccactgt atcaccacaa gcctcaacac caatatctca 1381 gagcacacca gtcttccctc ctgggtcact tcctatccca tcccagcctc agttttctca 1441 tgacattttt attccttccc caagtctgga agaacaatca aatgatggga agaaagatgg 1501 agatatgcat agttcatctt tgacagttga gtgttctaaa acttcagaga ttgaaccaaa 1561 gaattcccct gaggatcttg ggctatcttt gacaggggat tcttgcaagt tgatgctttc 1621 tacaagtgaa tatagtcagt ccccaaagat ggagagcttg agttctcaca gaattgatga 1681 agatggagaa aacacacaga ttgaggatac ggaacccatg tctccagttc tcaattctaa 1741 atttgttcct gctgaaaatg atagtatcct gatgaatcca gcacaggatg gtgaagtaca 1801 actgagtcag aatgatgaca aaacaaaggg agatgataca gacaccaggg atgacattag 1861 tattttagcc actggttgca agggcagaga agaaacggta gcagaagatg tttgtattga 1921 tctcacttgt gattcgggga gtcaggcagt tccgtcacca gctactcgat ctgaggcact 1981 ttctagtgtg ttagatcagg aggaagctat ggaaattaaa gaacaccatc cagaggaggg 2041 gtcttcaggg tctgaggtgg aagaaatccc tgagacacct tgtgaaagtc aaggagagga 2101 actcaaagaa gaaaatatgg agagtgttcc gttgcacctt tctctgactg aaactcagtc 2161 ccaagggttg tgtcttcaaa aggaaatgcc aaaaaaagaa tgctcagaag ctatggaagt 2221 tgaaaccagt gtgattagta ttgattcccc tcaaaagttg gcaatacttg accaagaatt 2281 ggaacataag gaacaggaag cttgggaaga agctacttca gaggactcca gtgttgtcat 2341 tgtagatgtg aaagagccat ctcccagagt tgatgtttct tgtgaacctt tggagggagt 2401 ggagaagtgc tcagattccc agtcatggga ggatattgct ccagaaatag aaccatgtgc 2461 tgagaataga ttagacacca aggaagaaaa gagtgtagaa tatgaaggag atctgaaatc 2521 agggactgca gaaacagaac ctgtagagca agattcttca cagccttcct tacctttagt 2581 gagagcagat gatcctttaa gacttgacca ggagttgcag cagccccaaa ctcaggagaa 2641 aacaagtaat tcattaacag aagactcaaa aatggctaat gcaaagcagc taagctcaga 2701 tgcagaggcc cagaagctgg ggaagccctc tgcccatgcc tcacaaagct tctgtgaaag 2761 ttctagtgaa accccatttc atttcacttt gcctaaagaa ggtgatatca tcccaccatt 2821 gactggtgca accccacctc ttattgggca cctaaaattg gagcccaaga gacacagtac 2881 tcctattggt attagcaact atccagaaag caccatagca accagtgatg tcatgtctga 2941 aagcatggtg gagacccatg atcccatact tgggagtgga aaaggggatt ctggggctgc 3001 cccagacgtg gatgataaat tatgtctaag aatgaaactg gttagtcctg agactgaggc 3061 gagtgaagag tctttgcagt tcaacctgga aaagcctgca actggtgaaa gaaaaaatgg 3121 atctactgct gttgctgagt ctgttgccag tccccagaag accatgtctg tgttgagctg 3181 tatctgtgaa gccaggcaag agaatgaggc tcgaagtgag gatcccccca ccacacccat 3241 cagggggaac ttgctccact ttccaagttc tcaaggagaa gaggagaaag aaaaattgga 3301 gggtgaccat acaatcaggc agagtcaaca gcctatgaag cccattagtc ctgtcaagga 3361 ccctgtttct cctgcttccc agaagatggt catacaaggg ccatccagtc ctcaaggaga 3421 ggcaatggtg acagatgtgc tagaagacca gaaagaagga cggagtacta ataaggaaaa 3481 tcctagtaag gccttgattg aaaggcccag ccaaaataac ataggaatcc aaaccatgga 3541 gtgttccttg agggtcccag aaactgtttc agcagcaacc cagactataa agaatgtgtg 3601 tgagcagggg accagtacag tggaccagaa ctttggaaag caagatgcca cagttcagac 3661 tgagaggggg agtggtgaga aaccagtcag tgctcctggg gatgatacag agtcgctcca 3721 tagccaggga gaagaagagt ttgatatgcc tcagcctcca catggccatg tcttacatcg 3781 tcacatgaga acaatccggg aagtacgcac acttgtcact cgtgtcatta cagatgtgta 3841 ttatgtggat ggaacagaag tagaaagaaa agtaactgag gagactgaag agccaattgt 3901 agagtgtcag gagtgtgaaa ctgaagtttc cccttcacag actgggggct cctcaggtga 3961 cctgggggat atcagctcct tctcctccaa ggcatccagc ttacaccgca catcaagtgg 4021 gacaagtctc tcagctatgc acagcagtgg aagctcaggg aaaggagccg gaccactcag 4081 agggaaaacc agcgggacag aacccgcaga ttttgcctta cccagctccc gaggaggccc 4141 aggaaaactg agtcctagaa aaggggtcag tcagacaggg acgccagtgt gtgaggagga 4201 tggtgatgca ggccttggca tcagacaggg agggaaggct ccagtcacgc ctcgtgggcg 4261 tgggcgaagg ggccgcccac cttctcggac cactggaacc agagaaacag ctgtgcctgg 4321 ccccttgggc atagaggaca tttcacctaa cttgtcacca gatgataaat ccttcagccg 4381 tgtcgtgccc cgagtgccag actccaccag acgaacagat gtgggtgctg gtgctttgcg 4441 tcgtagtgac tctccagaaa ttcctttcca ggctgctgct ggcccttctg atggcttaga 4501 tgcctcctct ccaggaaata gctttgtagg gctccgtgtt gtagccaagt ggtcatccaa 4561 tggctacttt tactctggga aaatcacacg agatgtcgga gctgggaagt ataaattgct 4621 ctttgatgat gggtacgaat gtgatgtgtt gggcaaagac attctgttat gtgaccccat 4681 cccgctggac actgaagtga cggccctctc ggaggatgag tatttcagtg caggagtggt 4741 gaaaggacat aggaaggagt ctggggaact gtactacagc attgaaaaag aaggccaaag 4801 aaagtggtat aagcgaatgg ctgtcatcct gtccttggag caaggaaaca gactgagaga 4861 gcagtatggg cttggcccct atgaagcagt aacacctctt acaaaggcag cagatatcag 4921 cttagacaat ttggtggaag ggaagcggaa acggcgcagt aacgtcagct ccccagccac 4981 ccctactgcc tccagtagca gcagcacaac ccctacccga aagatcacag aaagtcctcg 5041 tgcctccatg ggagttctct caggcaaaag aaaacttatc acttctgaag aggaacggtc 5101 ccctgccaag cgaggtcgca agtctgccac agtaaaacct ggtgcagtag gggcaggaga 5161 gtttgtgagc ccctgtgaga gtggagacaa caccggtgaa ccctctgccc tggaagagca 5221 gagagggcct ttgcctctca acaagacctt gtttctgggc tacgcatttc tccttaccat 5281 ggccacaacc agtgacaagt tggccagccg ctccaaactg ccagatggac cctactggaa 5341 gcagtgaaga agaggaggaa tttttggaaa ttcctccttt caacaagcag tatacagaat 5401 cccagcttcg agcaggagct ggctatatcc ttgaagattt caatgaagcc cagtgtaaca 5461 cagcttacca gtgtcttcta attgcggatc agcattgtcg aacccggaag tacttcctgt 5521 gccttgccag tgggattcct tgtgtgtctc atgtctgggt ccatgatagt tgccatgcca 5581 accagctcca gaactaccgt aattatctgt tgccagctgg gtacagcctt gaggagcaaa 5641 gaattctgga ctggcaaccc cgtgaaaaTC CTTTCCAGAA TCTGAAGGTA CTCTTGGTAT 5701 CAGACCAACA GCAGAACTTC CTGGAGCTCT GGTCTGAGAT CCTCATGACT GGTGGTGCAG 5761 CCTCTGTGAA GCAGCACCAT TCAAGTGCCC ATAACAAAGA TATTGCTTTA GGGGTATTTG 5821 ATGTGGTGGT GACGGACCCC TCATGCCCAG CCTCGGTGCT GAAGTGTGCT GAAGCATTGC 5881 AGCTGCCTGT GGTGTCACAA GAGTGGGTGA TCCAGTGCCT CATTGTTGGG GAGAGAATTG 5941 GATTCAAGCA GCATCCAAAA TATAAACACG ATTATGTTTC TCACTTGCCA ACTTTCTTGT 6001 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 6061 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 6121 GAAAGGACGA CCCGCTAGCA CTCTAGGATC TGGAACGCGT TAAGTCgaca atcaacctct 6181 ggattacaaa atttgtgaaa gatt