Construct: ORF TRCN0000465905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016589.1_s317c1
- Derived from:
- BRDN0000395095
- DNA Barcode:
- TAATCAAATAAGTATCCCCGATCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DIAPH1 (1729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1729 | DIAPH1 | diaphanous related formin 1 | NM_001079812.3 | 99.6% | 90% | (many diffs) |
| 2 | human | 1729 | DIAPH1 | diaphanous related formin 1 | NM_005219.5 | 98.9% | 89.4% | (many diffs) |
| 3 | human | 1729 | DIAPH1 | diaphanous related formin 1 | XM_024454385.1 | 96.4% | 87.2% | (many diffs) |
| 4 | human | 1729 | DIAPH1 | diaphanous related formin 1 | XM_024454384.1 | 95.8% | 86.6% | (many diffs) |
| 5 | human | 1729 | DIAPH1 | diaphanous related formin 1 | NM_001314007.2 | 94.4% | 90.9% | (many diffs) |
| 6 | human | 1729 | DIAPH1 | diaphanous related formin 1 | XM_024454387.1 | 94.3% | 84.6% | (many diffs) |
| 7 | human | 1729 | DIAPH1 | diaphanous related formin 1 | XM_024454386.1 | 93.2% | 84.4% | (many diffs) |
| 8 | mouse | 13367 | Diaph1 | diaphanous related formin 1 | NM_007858.4 | 86.1% | 81.6% | (many diffs) |
| 9 | mouse | 13367 | Diaph1 | diaphanous related formin 1 | NM_001305980.2 | 85.5% | 81.1% | (many diffs) |
| 10 | mouse | 13367 | Diaph1 | diaphanous related formin 1 | NM_001305981.2 | 83.6% | 79% | (many diffs) |
| 11 | mouse | 13367 | Diaph1 | diaphanous related formin 1 | XM_011246826.2 | 55.2% | 62.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3522
- ORF length:
- 3453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccgccc ggcgggagac aggggcccgg ccgcgggacc cgggacaaga 121 agaagggccg gagcccagat gagctgccct cggcgggcgg cgacggcggc aaatctaaga 181 aatttctgga gagatttacc agcatgagaa ttaagaagga gaaggaaaag cccaattctg 241 ctcatagaaa ttcttctgca tcatatgggg atgatcccac agcacagtca ttgcaagatg 301 tttcagatga acaagtgctg gttctctttg aacagatgct gctggatatg aacctgaatg 361 aggagaaaca gcaacctttg agggagaagg acatcatcat caagagggag atggtgtccc 421 aatacttgta cacctccaag gctggcatga gccagaagga gagctctaag tctgccatga 481 tgtatattca ggagttgagg tcaggcttgc gggatatgcc tctgctcagc tgcctggagt 541 cccttcgtgt gtctctcaac aacaaccctg tcagttgggt gcaaacattt ggtgctgaag 601 gcttggcctc cttattggac attcttaaac gacttcatga tgagaaagaa gagactgctg 661 ggagttacga tagccggaac aagcatgaga tcattcgctg cttgaaagct tttatgaaca 721 acaagtttgg aatcaagacc atgttggaga cagaagaagg aatcctactg ctggtcagag 781 ccatggatcc tgctgttccc aacatgatga ttgatgcagc taagctgctt tctgctcttt 841 gtattctacc gcagccagag gacatgaatg aaagggtttt ggaggcaatg acagaaagag 901 ctgagatgga tgaagtggaa cgtttccagc cgctgctgga tggattaaaa agtggaacca 961 ctattgcact gaaggttgga tgcctacagc tgatcaatgc tctcatcaca ccagcggagg 1021 aacttgactt ccgagttcac atcagaagtg aactgatgcg tttggggcta catcaggtgt 1081 tgcaggacct tcgagagatt gaaaatgaag atatgagagt gcaactaaat gtgtttgatg 1141 aacaagggga agaggattcc tatgacctga agggacggct ggatgacatt cgcatggaga 1201 tggatgactt taatgaagtc tttcagattc tcttaaacac agtgaaggat tcaaaggcag 1261 agccacactt cctttccatc ctgcagcact tactcttggt ccgaaatgac tatgaggcca 1321 gacctcagta ctataagttg attgaagaat gtatttccca gatagttctg cacaagaacg 1381 gggctgatcc tgacttcaag tgccggcacc tccagattga gattgaggga ttaattgatc 1441 aaatgattga taagacaaag gtggagaaat ctgaagccaa agctgcagag ctggaaaaga 1501 agttggactc agagttaaca gcccgacatg agctacaggt ggaaatgaaa aagatggaaa 1561 gtgactttga gcagaagctt caagatcttc agggagaaaa agatgcactg cattctgaaa 1621 agcagcaaat tgccacagag aaacaggacc tggaagcaga ggtgtcccag ctcacaggag 1681 aggttgccaa gctgacaaag gaactggaag atgccaagaa agaaatggct tccctctctg 1741 cggcagctat tactgtacct ccttctgttc ctagtcgtgc tcctgttccc cctgcccctc 1801 ctttacctgg tgactctggc actattattc caccaccacc tgctcctggg gatagtacca 1861 ctcctcctcc tcctcctcct cctcctcctc cacctccttt gcctgggggt gtttgcatct 1921 cctcaccccc ttctttacct ggaggtactg ctatctctcc accccctcct ttgtctgggg 1981 atgctaccat ccctccaccc cctcctttgc ctgagggtgt tggcatccct tcaccctctt 2041 ctttgcctgg aggtactgcc atccccccac ctcctccttt gcctgggagt gctagaatcc 2101 ccccaccacc acctcctttg cctgggagtg ctggaattcc ccccccacct cctcccttgc 2161 ctggagaagc aggaatgcca cctcctcctc cccctcttcc tggtggtcct ggaatccctc 2221 cacctcctcc atttcccgga ggccctggca ttcctccacc tccacccgga atgggtatgc 2281 ctccacctcc cccatttgga tttggagttc ctgcagcccc agttctgcca tttggattaa 2341 cccccaaaaa gctttataag ccagaggtgc agctccggag gccaaactgg tccaagcttg 2401 tggctgagga cctctcccag gactgcttct ggacaaaggt gaaggaggac cgctttgaga 2461 acaatgaact tttcgccaaa cttaccctta ccttctctgc ccagaccaag acttccaaag 2521 ccaagaagga tcaagaaggt ggagaagaaa agaaatctgt gcaaaagaaa aaagtaaaag 2581 agttaaaggt gttggattca aagacagccc agaatctctc aatctttttg ggttccttcc 2641 gcatgcccta tcaagagatt aagaatgtca tcctggaggt gaatgaggct gttctgactg 2701 agtctatgat ccagaacctc attaagcaaa tgccagagcc agagcagtta aaaatgcttt 2761 ctgaactgaa ggatgaatat gatgacctgg ctgagtcaga gcagtttggc gtggtgatgg 2821 gcactgtgcc ccgactgcgg cctcgcctca atgccattct cttcaagcta caattcagcg 2881 agcaagtgga gaatatcaag ccagagattg tgtctgtcac tgctgcatgt gaggagttac 2941 gtaagagtga gagcttttcc aatctcctag agattacctt gcttgttgga aattacatga 3001 atgctggctc cagaaatgct ggtgcttttg gcttcaatat cagcttcctc tgtaagcttc 3061 gagacaccaa gtccacagat cagaagatga cgttgttaca cttcttggct gagttgtgtg 3121 agaatgacta tcccgatgtc ctcaagtttc cagacgagct tgcccatgtg gagaaagcca 3181 gccgagtttc tgctgaaaac ttgcaaaaga acctagatca gatgaagaaa caaatttctg 3241 atgtggaacg tgatgttcag aatttcccag ctgccacaga tgaaaaagac aagtttgttg 3301 aaaaaatgac cagctttgtg aaggatgcac aggaacagta taacaagctg cggatgatgc 3361 attctaacat ggagaccctc tataaggagc tgggcgagta cttcctcttt gaccccaaga 3421 agttgtctgt tgaagaattt ttcatggatc ttcacaattt tcggaatatg tttttgcaag 3481 cagtcaagga GGAGCCGCCC GGCGGGAGAC AGAAGAAAAG ATGAGGCGAG CAAAACTAGC 3541 CAAGGAGAAG GCAGAGAAGG AGCGGCTAGA GAAGCAGCAG AAGAGAGAGC AACTCATAGA 3601 CATGAATGCA GAGGGCGATG AGACAGGTGT GATGGACAGT CTTCTAGAAG CCCTGCAGTC 3661 AGGGGCAGCA TTCCGACGGA AGAGAGGGCC CCGTCAAGCC AACAGGAAGG CCGGGTGTGC 3721 AGTCACATCT CTGCTAGCTT CGGAGCTGAC CAAGGATGAT GCCATGGCTG CTGTTCCTGC 3781 CAAGGTGTCC AAGAACAGTG AGACATTCCC CACAATCCTT GAGGAAGCCA AGGAGTTGGT 3841 TGGCCGTGCA AGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 3901 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 3961 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATCAAATAA GTATCCCCGA 4021 TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att