Construct: ORF TRCN0000465915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003307.3_s317c1
- Derived from:
- ccsbBroadEn_14808
- DNA Barcode:
- AACCGTCGGGCCCACCATGAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K2 (5605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5605 | MAP2K2 | mitogen-activated protein k... | NM_030662.3 | 99.8% | 100% | 453C>T;660C>A |
| 2 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_006722799.2 | 76.5% | 76.7% | 453C>T;660C>A;705_706ins279 |
| 3 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026989.1 | 75.2% | 67.6% | (many diffs) |
| 4 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026990.1 | 70.7% | 50% | (many diffs) |
| 5 | human | 407835 | LOC407835 | mitogen-activated protein k... | NR_002144.1 | 65.9% | (many diffs) | |
| 6 | human | 5605 | MAP2K2 | mitogen-activated protein k... | XM_017026991.1 | 61.5% | 60.2% | (many diffs) |
| 7 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_001347144.1 | 87.2% | 94.2% | (many diffs) |
| 8 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | NM_023138.5 | 87% | 94% | (many diffs) |
| 9 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513684.3 | 45.1% | 48.2% | (many diffs) |
| 10 | mouse | 26396 | Map2k2 | mitogen-activated protein k... | XM_006513683.3 | 45% | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggcccggagg aagccggtgc tgccggcgct caccatcaac cctaccatcg 121 ccgagggccc atcccctacc agcgagggcg cctccgaggc aaacctggtg gacctgcaga 181 agaagctgga ggagctggaa cttgacgagc agcagaagaa gcggctggaa gcctttctca 241 cccagaaagc caaggtcggc gaactcaaag acgatgactt cgaaaggatc tcagagctgg 301 gcgcgggcaa cggcggggtg gtcaccaaag tccagcacag accctcgggc ctcatcatgg 361 ccaggaagct gatccacctt gagatcaagc cggccatccg gaaccagatc atccgcgagc 421 tgcaggtcct gcacgaatgc aactcgccgt acatcgtggg cttctacggg gccttctaca 481 gtgacgggga gatcagcatt tgcatggaac acatggatgg cggctccctg gaccaggtgc 541 tgaaagaggc caagaggatt cccgaggaga tcctggggaa agtcagcatc gcggttctcc 601 ggggcttggc gtacctccga gagaagcacc agatcatgca ccgagatgtg aagccctcca 661 acatcctcgt gaactctaga ggggagatca agctgtgtga cttcggggtg agcggccagc 721 tcatagactc catggccaac tccttcgtgg gcacgcgctc ctacatggct ccggagcggt 781 tgcagggcac acattactcg gtgcagtcgg acatctggag catgggcctg tccctggtgg 841 agctggccgt cggaaggtac cccatccccc cgcccgacgc caaagagctg gaggccatct 901 ttggccggcc cgtggtcgac ggggaagaag gagagcctca cagcatctcg cctcggccga 961 ggccccccgg gcgccccgtc agcggtcacg ggatggatag ccggcctgcc atggccatct 1021 ttgaactcct ggactatatt gtgaacgagc cacctccTAA GCTGCCCAAC GGTGTGTTCA 1081 CCCCCGACTT CCAGGAGTTT GTCAATAAAT GCCTCATCAA GAACCCAGCG GAGCGGGCGG 1141 ACCTGAAGAT GCTCACAAAC CACACCTTCA TCAAGCGGTC CGAGGTGGAA GAAGTGGATT 1201 TTGCCGGCTG GTTGTGTAAA ACCCTGCGGC TGAACCAGCC CGGCACACCC ACGCGCACCG 1261 CCGTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACCGTCGG GCCCACCATG AAATTACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt