Construct: ORF TRCN0000466223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012851.2_s317c1
- Derived from:
- ccsbBroadEn_13986
- DNA Barcode:
- ACCATACTTGTCTATGTCGTCGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K12 (7786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466223
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_005269140.2 | 79.7% | 81.1% | 1359_1359delGins345 |
| 2 | human | 7786 | MAP3K12 | mitogen-activated protein k... | NM_001193511.2 | 59.3% | 57.7% | (many diffs) |
| 3 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_005269138.4 | 59.3% | 57.7% | (many diffs) |
| 4 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_006719588.4 | 59.3% | 57.7% | (many diffs) |
| 5 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_011538725.3 | 59.3% | 57.7% | (many diffs) |
| 6 | human | 7786 | MAP3K12 | mitogen-activated protein k... | NM_006301.4 | 55.7% | 54% | (many diffs) |
| 7 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_017019956.2 | 55.7% | 54% | (many diffs) |
| 8 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XR_001781538.1 | 58.3% | (many diffs) | |
| 9 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | NM_001163643.1 | 55.7% | 57.7% | (many diffs) |
| 10 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | NM_009582.4 | 55.7% | 57.7% | (many diffs) |
| 11 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XM_017316624.1 | 55.7% | 57.7% | (many diffs) |
| 12 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XM_017316625.1 | 55.7% | 57.7% | (many diffs) |
| 13 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XR_001781537.1 | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttgcctccat gagacccgaa caccctctcc ttcctttggg ggctttgtgt 121 ctaccctaag tgaggcatcc atgcgcaagc tggacccaga cacttctgac tgcactcccg 181 agaaggacct gacgcctacc cagtgtgtac ttcgagatgt ggtacccctt ggtgggcagg 241 gtgggggagg gcccagcccc tccccaggtg gagagccgcc ccctgagcct tttgccaaca 301 gtgtcctgca gctacatgag caggatgcag ggggcccagg gggagcagct gggtcacctg 361 agagtcgggc atccagagtt cgagctgacg aggtgcgact gcagtgccag agtggcagtg 421 gcttccttga gggcctcttt ggctgcctgc gccctgtctg gaccatgatt ggcaaagcct 481 actccactga gcacaagcag cagcaggaag acctttggga ggtccccttt gaggaaatcc 541 tggacctgca gtgggtgggc tcaggggccc agggtgctgt cttcctgggg cgcttccacg 601 gggaggaggt ggctgtgaag aaggtgcgag acctcaaaga aaccgacatc aagcacttgc 661 gaaagctgaa gcaccccaac atcatcactt tcaagggtgt gtgcacccag gctccctgct 721 actgcatcct catggagttc tgcgcccagg gccagctgta tgaggtactg cgggctggcc 781 gccctgtcac cccctcctta ctggttgact ggtccatggg catcgctggt ggcatgaact 841 acctgcacct gcacaagatt atccacaggg atctcaagtc acccaacatg ctaatcacct 901 acgacgatgt ggtgaagatc tcagattttg gcacttccaa ggagctgagt gacaagagca 961 ccaagatgtc ctttgcaggg acagtagcct ggatggcccc tgaggtgatc cgcaatgaac 1021 ctgtgtctga gaaggtcgac atctggtcct ttggcgtggt gctatgggaa ctgctgactg 1081 gtgagatccc ctacaaagac gtagattcct cagccattat ctggggtgtg ggaagcaaca 1141 gtctccatct gcccgtgccc tccagttgcc cagatggttt caagatcctg cttcgccagt 1201 gctggaatag caaaccacga aatcgcccat cattccgaca gatcctgctg catctggaca 1261 ttgcctcagc tgatgtactc tccacacccc aggagactta ctttaagtcc caggcagagt 1321 ggcgggaaga agtaaaactg cactttgaaa agattaagtc agaagggacc tgtctgcacc 1381 gcctagaaga ggaactggtg atgaggagga gggaggagcT CAGACACGCC CTGGACATCA 1441 GGGAGCACTA TGAAAGGAAG CTGGAGAGAG CCAACAACCT GTATATGGAA CTTAATGCCC 1501 TCATGTTGCA GCTGGAACTC AAGGAGAGGG AGCTGCTCAG GCGAGAGCAA GCTTTAGAGC 1561 GGAGGTGCCC AGGCCTGCTG AAGCCACACC CTTCCCGGGG CCCCCAGCTT CCCTCCCTCC 1621 ATGAAAGCCA CTCGTATTCC TTGTACATAG AGAAATATTT ATATAAATTA TATATATATA 1681 TACATATATA TATATATATG CGCCACATAA TCAACAGAAA GATGGGGCTG TCCCAGCCGT 1741 AAGTCAGGCT CGAGGGAGAC TGATCCCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT 1801 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 1861 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCATA 1921 CTTGTCTATG TCGTCGGCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 1981 gaaagatt