Construct: ORF TRCN0000466263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006379.1_s317c1
- Derived from:
- ccsbBroadEn_00603
- DNA Barcode:
- GTCGTATTTAGCCCTAATACTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAGE1 (2543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2543 | GAGE1 | G antigen 1 | XM_011543896.2 | 82.2% | 76.5% | (many diffs) |
| 2 | human | 2543 | GAGE1 | G antigen 1 | NM_001040663.2 | 81.9% | 80% | (many diffs) |
| 3 | human | 2576 | GAGE4 | G antigen 4 | NM_001474.1 | 81.8% | 80% | (many diffs) |
| 4 | human | 100008586 | GAGE12F | G antigen 12F | NM_001098405.2 | 81.5% | 79.2% | (many diffs) |
| 5 | human | 645073 | GAGE12G | G antigen 12G | NM_001098409.2 | 81.5% | 79.2% | (many diffs) |
| 6 | human | 729428 | GAGE12B | G antigen 12B | NM_001127345.2 | 81.5% | 79.2% | (many diffs) |
| 7 | human | 2577 | GAGE5 | G antigen 5 | NM_001475.1 | 81.5% | 79.2% | (many diffs) |
| 8 | human | 26748 | GAGE12I | G antigen 12I | NM_001477.1 | 81.5% | 79.2% | (many diffs) |
| 9 | human | 2579 | GAGE7 | G antigen 7 | NM_021123.3 | 81.5% | 79.2% | (many diffs) |
| 10 | human | 729396 | GAGE12J | G antigen 12J | NM_001098406.2 | 81.3% | 79.2% | (many diffs) |
| 11 | human | 729422 | GAGE12C | G antigen 12C | NM_001098408.1 | 81.3% | 78.5% | (many diffs) |
| 12 | human | 729431 | GAGE12E | G antigen 12E | NM_001098418.1 | 81.3% | 78.5% | (many diffs) |
| 13 | human | 100132399 | GAGE12D | G antigen 12D | NM_001127199.1 | 81.3% | 78.5% | (many diffs) |
| 14 | human | 2578 | GAGE6 | G antigen 6 | NM_001476.1 | 81.3% | 78.5% | (many diffs) |
| 15 | human | 645051 | GAGE13 | G antigen 13 | NM_001098412.2 | 81.1% | 79.2% | (many diffs) |
| 16 | human | 729442 | GAGE12H | G antigen 12H | NM_001098410.1 | 81.1% | 77.8% | (many diffs) |
| 17 | human | 729408 | GAGE2D | G antigen 2D | NM_001098407.1 | 80.1% | 77.8% | (many diffs) |
| 18 | human | 26749 | GAGE2E | G antigen 2E | NM_001127200.3 | 80.1% | 77.8% | (many diffs) |
| 19 | human | 100101629 | GAGE8 | G antigen 8 | NM_012196.1 | 80.1% | 77.8% | (many diffs) |
| 20 | human | 645037 | GAGE2B | G antigen 2B | NM_001098411.3 | 79.9% | 77.1% | (many diffs) |
| 21 | human | 2574 | GAGE2C | G antigen 2C | NM_001472.2 | 79.9% | 77.1% | (many diffs) |
| 22 | human | 729447 | GAGE2A | G antigen 2A | NM_001127212.1 | 79.6% | 76.4% | (many diffs) |
| 23 | human | 102724473 | GAGE10 | G antigen 10 | NM_001098413.3 | 77.9% | 72.8% | (many diffs) |
| 24 | human | 102724473 | GAGE10 | G antigen 10 | XM_024452325.1 | 65.6% | 57.8% | (many diffs) |
| 25 | human | 645073 | GAGE12G | G antigen 12G | NM_001354422.1 | 53.4% | 51.7% | (many diffs) |
| 26 | human | 2543 | GAGE1 | G antigen 1 | NR_102272.1 | 13.2% | 1_123del;541_3157del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttGGCGAGGA AGATCGACCT ATTATTGGCC TAGACCAAGG CGCTATGTAC 121 AGCCTCCTGA AATGATTGGG CCTATGCGGC CCGAGCAGTT CAGTGATGAA GTGGAACCAG 181 CAACACCTGA AGAAGGGGAA CCAGCAACTC AACGTCAGGA TCCTGCAGCT GCTCAGGAGG 241 GAGAGGATGA GGGAGCATCT GCAGGTCAAG GGCCGAAGCC TGAAGCTGAT AGCCAGGAAC 301 AGGGTCACCC ACAGACTGGG TGTGAGTGTG AAGATGGTCC TGATGGGCAG GAGATGGACC 361 CGCCAAATCC AGAGGAGGTG AAAACGCCTG AAGAAGAGAT GAGGTCTCAC TATGTTGCCC 421 AGACTGGGAT TCTCTGGCTT TTAATGAACA ATTGCTTCTT AAATCTTTCC CCACGGAAAC 481 CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGAGT CGTATTTAGC CCTAATACTC GGACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt