Construct: ORF TRCN0000466288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013088.1_s317c1
- Derived from:
- ccsbBroadEn_06183
- DNA Barcode:
- AAGTCCGCGGCTTAGCAAAGCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EWSR1 (2130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | NM_001163287.2 | 99.9% | 99.7% | 968G>T |
| 2 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028660.2 | 57.3% | 52.4% | (many diffs) |
| 3 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028656.2 | 56% | 52.7% | (many diffs) |
| 4 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028653.2 | 55.7% | 51% | (many diffs) |
| 5 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028651.2 | 55.6% | 52.4% | (many diffs) |
| 6 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028652.2 | 55.6% | 50.9% | (many diffs) |
| 7 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028650.2 | 55.6% | 52.3% | (many diffs) |
| 8 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_024452181.1 | 54.8% | 49.7% | (many diffs) |
| 9 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028649.2 | 54.4% | 51.2% | (many diffs) |
| 10 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028648.2 | 53.6% | 49.1% | (many diffs) |
| 11 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_024452180.1 | 53.5% | 48.5% | (many diffs) |
| 12 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011530000.2 | 53.5% | 50.3% | (many diffs) |
| 13 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028647.2 | 52.6% | 48.2% | (many diffs) |
| 14 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028645.2 | 52.5% | 49.4% | (many diffs) |
| 15 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028646.2 | 52.5% | 48.1% | (many diffs) |
| 16 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011529999.3 | 52.4% | 49.3% | (many diffs) |
| 17 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | NM_001163285.2 | 52.3% | 47.9% | (many diffs) |
| 18 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | NM_005243.4 | 52.2% | 49.1% | (many diffs) |
| 19 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011529998.2 | 52.2% | 47.8% | (many diffs) |
| 20 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011529997.2 | 52.1% | 49% | (many diffs) |
| 21 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011530002.3 | 52% | 47.2% | (many diffs) |
| 22 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | NM_013986.4 | 51.8% | 47.5% | (many diffs) |
| 23 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028644.2 | 51.2% | 47% | (many diffs) |
| 24 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_005261389.4 | 51.2% | 48.2% | (many diffs) |
| 25 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011529996.3 | 51.2% | 46.9% | (many diffs) |
| 26 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011529995.3 | 51.1% | 48.1% | (many diffs) |
| 27 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028663.1 | 46.6% | 42.2% | (many diffs) |
| 28 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028662.2 | 44.9% | 40.6% | (many diffs) |
| 29 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028659.1 | 43.7% | 39.6% | (many diffs) |
| 30 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | NM_001163286.2 | 43.7% | 40.7% | (many diffs) |
| 31 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028658.1 | 43.7% | 39.6% | (many diffs) |
| 32 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028655.1 | 42.9% | 38.9% | (many diffs) |
| 33 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_005261390.4 | 42.8% | 39.9% | (many diffs) |
| 34 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028654.1 | 42.8% | 38.8% | (many diffs) |
| 35 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_011530001.2 | 42.8% | 39.9% | (many diffs) |
| 36 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028661.2 | 41.6% | 37% | (many diffs) |
| 37 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XM_017028657.2 | 40.7% | 37.3% | (many diffs) |
| 38 | human | 2130 | EWSR1 | EWS RNA binding protein 1 | XR_002958676.1 | 27.2% | (many diffs) | |
| 39 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | NM_001283063.1 | 86.3% | 88.7% | (many diffs) |
| 40 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | NM_001283062.1 | 51.7% | 48.8% | (many diffs) |
| 41 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | NM_007968.3 | 49.6% | 46.4% | (many diffs) |
| 42 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | NM_001283061.1 | 49.5% | 47.6% | (many diffs) |
| 43 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XM_006514512.3 | 49.2% | 46% | (many diffs) |
| 44 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XM_017314255.1 | 49.2% | 46% | (many diffs) |
| 45 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XM_006514510.2 | 49.1% | 47.2% | (many diffs) |
| 46 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XM_006514511.2 | 49.1% | 47.2% | (many diffs) |
| 47 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XM_006514509.2 | 48.7% | 45.6% | (many diffs) |
| 48 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XM_006514508.2 | 48.7% | 46.8% | (many diffs) |
| 49 | mouse | 14030 | Ewsr1 | Ewing sarcoma breakpoint re... | XR_001779874.1 | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtccacggat tacagtacct atagccaagc tgcagcgcag cagggctaca 121 gtgcttacac cgcccagccc actcaaggat atgcacagac cacccaggca tatgggcaac 181 aaagctatgg aacctatgga cagcccactg atgtcagcta tacccaggct cagaccactg 241 caacctatgg gcagaccgcc tatgcaactt cttatggaca gcctcccact ggttatacta 301 ctccaactgc cccccaggca tacagccagc ctgtccaggg gtatggcact ggtgcttatg 361 ataccaccac tgctacagtc accaccaccc aggcctccta tgcagctcag tctgcatatg 421 gcactcagcc tgcttatcca gcctatgggc agcagccagc agccactgca cctacaagac 481 cgcaggatgg aaacaagccc actgagacta gtcaacctca atctagcaca gggggttaca 541 accagcccag cctaggatat ggacagagta actacagtta tccccaggta cctgggagct 601 accccatgca gccagtcact gcacctccat cctaccctcc taccagctat tcctctacac 661 agccgactag ttatgatcag agcagttact ctcagcagaa cacctatggg CAACCGAGCA 721 GCTATGGACA GCAGAGTAGC TATGGTCAAC AAAGCAGCTA TGGGCAGCAG CCTCCCACTA 781 GTTACCCACC CCAAACTGGA TCCTACAGCC AAGCTCCAAG TCAATATAGC CAACAGAGCA 841 GCAGCTACGG GCAGCAGAGT TCATTCCGAC AGGACCACCC CAGTAGCATG GGTGTTTATG 901 GGCAGGAGTC TGGAGGATTT TCCGGACCAG GAGAGAACCG GAGCATGAGT GGCCCTGATA 961 ACCGGGGCAG GGGAAGAGGG GGATTTGATC GTGGAGGCAT GAGCAGAGGT GGGCGGGGAG 1021 GAGGACGCGG TGTAATGGGG TTACAAAGTG AGAGCCTTGT ATACACTTCA ATACTTAAAA 1081 AGTACCCGTA CTCAGTACTC AGCCGGCAGC ATAATGAAAA GTGGGACTAC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAAAGTCCG CGGCTTAGCA AAGCCGGACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt