Construct: ORF TRCN0000466573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013082.1_s317c1
- Derived from:
- ccsbBroadEn_14111
- DNA Barcode:
- TTGTGTATGCCTCTTGCCACGACC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAGE12I (26748)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100008586 | GAGE12F | G antigen 12F | NM_001098405.2 | 99.7% | 95.7% | 335delA |
| 2 | human | 645073 | GAGE12G | G antigen 12G | NM_001098409.2 | 99.7% | 95.7% | 335delA |
| 3 | human | 729428 | GAGE12B | G antigen 12B | NM_001127345.2 | 99.7% | 95.7% | 335delA |
| 4 | human | 26748 | GAGE12I | G antigen 12I | NM_001477.1 | 99.7% | 95.7% | 335delA |
| 5 | human | 2579 | GAGE7 | G antigen 7 | NM_021123.3 | 99.7% | 95.7% | 335delA |
| 6 | human | 729422 | GAGE12C | G antigen 12C | NM_001098408.1 | 99.4% | 94.8% | 148T>C;335delA |
| 7 | human | 729431 | GAGE12E | G antigen 12E | NM_001098418.1 | 99.4% | 94.8% | 148T>C;335delA |
| 8 | human | 100132399 | GAGE12D | G antigen 12D | NM_001127199.1 | 99.4% | 94.8% | 148T>C;335delA |
| 9 | human | 2576 | GAGE4 | G antigen 4 | NM_001474.1 | 99.4% | 94.8% | 223G>C;335delA |
| 10 | human | 2543 | GAGE1 | G antigen 1 | NM_001040663.2 | 99.1% | 94.8% | 223G>C;337_338delGGinsA |
| 11 | human | 729442 | GAGE12H | G antigen 12H | NM_001098410.1 | 99.1% | 94% | 148T>C;172A>G;335delA |
| 12 | human | 2577 | GAGE5 | G antigen 5 | NM_001475.1 | 99.1% | 94% | 67G>A;223G>C;335delA |
| 13 | human | 729396 | GAGE12J | G antigen 12J | NM_001098406.2 | 98.8% | 94% | (many diffs) |
| 14 | human | 2578 | GAGE6 | G antigen 6 | NM_001476.1 | 98.8% | 93.1% | (many diffs) |
| 15 | human | 645051 | GAGE13 | G antigen 13 | NM_001098412.2 | 98.5% | 94% | (many diffs) |
| 16 | human | 645037 | GAGE2B | G antigen 2B | NM_001098411.3 | 97.7% | 93.1% | (many diffs) |
| 17 | human | 2574 | GAGE2C | G antigen 2C | NM_001472.2 | 97.7% | 93.1% | (many diffs) |
| 18 | human | 729408 | GAGE2D | G antigen 2D | NM_001098407.1 | 97.4% | 92.3% | (many diffs) |
| 19 | human | 26749 | GAGE2E | G antigen 2E | NM_001127200.3 | 97.4% | 92.3% | (many diffs) |
| 20 | human | 729447 | GAGE2A | G antigen 2A | NM_001127212.1 | 97.4% | 92.3% | (many diffs) |
| 21 | human | 100101629 | GAGE8 | G antigen 8 | NM_012196.1 | 97.4% | 92.3% | (many diffs) |
| 22 | human | 102724473 | GAGE10 | G antigen 10 | NM_001098413.3 | 94.8% | 86.3% | (many diffs) |
| 23 | human | 2543 | GAGE1 | G antigen 1 | XM_011543896.2 | 90.2% | 93.2% | 223G>C;333_334insGAAAGCAATCACAG;337_357del |
| 24 | human | 102724473 | GAGE10 | G antigen 10 | XM_024452325.1 | 80% | 68.3% | (many diffs) |
| 25 | human | 645073 | GAGE12G | G antigen 12G | NM_001354422.1 | 62.7% | 61.5% | (many diffs) |
| 26 | human | 2543 | GAGE1 | G antigen 1 | NR_102272.1 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 441
- ORF length:
- 351
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGTTGGCG AGGAAGATCG ACCTATTATT 121 GGCCTAGACC AAGGCGCTAT GTACAGCCTC CTGAAATGAT TGGGCCTATG CGGCCCGAGC 181 AGTTCAGTGA TGAAGTGGAA CCAGCAACAC CTGAAGAAGG GGAACCAGCA ACTCAACGTC 241 AGGATCCTGC AGCTGCTCAG GAGGGAGAGG ATGAGGGAGC ATCTGCAGGT CAAGGGCCGA 301 AGCCTGAAGC TCATAGCCAG GAACAGGGTC ACCCACAGAC TGGGTGTGAG TGTGAAGATG 361 GTCCTGATGG GCAGGAGATG GACCCGCCAA ATCCAGAGGA GGTGAAAACG CCTGAAGAAG 421 GTGAAAGCAA TCACAGTGTT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 481 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 541 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTGTG TATGCCTCTT 601 GCCACGACCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt