Construct: ORF TRCN0000466777

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008009.1_s317c1
Derived from:
ccsbBroadEn_11060
DNA Barcode:
GTCTGTCAGTTTCCAATATTTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPSAP1 (5635)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466777
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5635 PRPSAP1 phosphoribosyl pyrophosphat... NM_002766.3 92.3% 92.4% 1_87del;1044A>G
2 human 5635 PRPSAP1 phosphoribosyl pyrophosphat... NM_001330503.2 79.1% 79.2% 0_1ins222;735A>G
3 human 5635 PRPSAP1 phosphoribosyl pyrophosphat... NM_001366236.1 79.1% 79.2% 0_1ins222;735A>G
4 human 5635 PRPSAP1 phosphoribosyl pyrophosphat... XR_001752566.2 40.9% (many diffs)
5 mouse 67763 Prpsap1 phosphoribosyl pyrophosphat... XM_006534018.2 86.6% 90.8% (many diffs)
6 mouse 67763 Prpsap1 phosphoribosyl pyrophosphat... XM_011249196.2 85.4% 89.5% (many diffs)
7 mouse 67763 Prpsap1 phosphoribosyl pyrophosphat... NM_026364.1 83.3% 91.6% (many diffs)
8 mouse 67763 Prpsap1 phosphoribosyl pyrophosphat... XM_006534017.3 82.3% 90.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1134
ORF length:
1068
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa cgccgctcgc accggctacc gagtcttctc ggccaactcc acggccgcct
121gcacggagct ggccaagcgc atcacagagc gccttggtgc tgaattgggg aagtctgttg
181tatatcaaga gaccaatgga gaaacaagag ttgaaataaa agaatctgtt cgtggccaag
241atattttcat tatacagaca atacccagag atgtgaatac agctgtgatg gagttgctca
301tcatggctta cgcactgaag actgcctgtg ccaggaacat tattggggtc atcccctact
361tcccctacag caagcagagc aagatgagga agaggggttc cattgtgtgc aagctgctag
421catccatgct ggcgaaagca ggtttaactc acattatcac tatggatctt catcaaaagg
481aaatacaagg ctttttcagc tttcctgtgg acaaccttag agcctcacct ttcctgcttc
541agtatatcca ggaagaaatt ccaaattaca gaaatgcagt cattgtagct aagtctcctg
601atgctgcaaa gagggcccag tcctatgcgg agagactgcg tctgggtttg gccgtcattc
661acggggaagc tcagtgcacg gaactggaca tggacgatgg tcgtcactcc ccgcctatgg
721TCAAAAATGC TACTGTGCAC CCAGGCCTGG AGTTGCCATT GATGATGGCC AAAGAGAAGC
781CACCGATAAC TGTAGTTGGA GATGTTGGAG GCCGCATCGC AATCATCGTG GATGACATTA
841TTGACGATGT GGAGAGTTTT GTTGCTGCCG CGGAGATCCT GAAAGAGAGA GGCGCCTATA
901AGATCTATGT TATGGCCACC CACGGCATCC TGTCTGCAGA GGCCCCTCGC CTGATTGAGG
961AGTCCTCCGT AGACGAGGTG GTGGTGACGA ATACTGTCCC TCATGAGGTT CAGAAGCTGC
1021AGTGTCCCAA GATAAAGACT GTGGATATCA GTTTGATTCT TTCTGAAGCC ATTCGGAGAA
1081TCCACAATGG AGAGTCCATG GCCTACCTTT TCCGAAACAT CACTGTGGAT GACTACCCAA
1141CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1201ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1261TATCTTGTGG AAAGGACGAG TCTGTCAGTT TCCAATATTT CTTACGCGTT AAGTCgacaa
1321tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)

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