Construct: ORF TRCN0000467028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002858.2_s317c1
- Derived from:
- ccsbBroadEn_03591
- DNA Barcode:
- CGACTAAGAACAGAGCAATGAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETNK1 (55500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_017019580.1 | 91.9% | 89.4% | (many diffs) |
| 2 | human | 55500 | ETNK1 | ethanolamine kinase 1 | NM_018638.5 | 80.3% | 80.3% | 0_1ins267 |
| 3 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_017019581.1 | 72.2% | 71% | (many diffs) |
| 4 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_005253420.4 | 56.6% | 53.3% | (many diffs) |
| 5 | human | 55500 | ETNK1 | ethanolamine kinase 1 | NM_001039481.2 | 35.1% | 32.7% | (many diffs) |
| 6 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XR_002957349.1 | 18.8% | 1_19del;986_1088del;1479_7192del | |
| 7 | mouse | 75320 | Etnk1 | ethanolamine kinase 1 | NM_029250.2 | 70.7% | 75.4% | (many diffs) |
| 8 | mouse | 75320 | Etnk1 | ethanolamine kinase 1 | XM_006507011.3 | 52.7% | 55.5% | (many diffs) |
| 9 | mouse | 75320 | Etnk1 | ethanolamine kinase 1 | XM_006507012.1 | 45.4% | 48.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ctgcggccgc ccgcggtcca gctccgacaa caggaatttt ctccgagagc 121 gggccgggct cagttcagct gctgtccaga cccggatcgg caacagtgcc gcctccagac 181 gttctcctgc cgctcgcccg cccgtcccag cgcccccagc cctcccgcga gggcgccccg 241 ggacggaagg atccaccagt ctgtcggcgc ccgccgttct cgtggtcgcc gtcgccgtcg 301 tcgtggtggt agtctccgcc gtcgcctggg ccatggccaa ttacatccac gtccctcccg 361 gctccccgga ggtgcccaag ctgaacgtca ccgttcagga tcaggaggag catcgctgcc 421 gggagggggc cctgagcctc ctgcaacacc tgcggcctca ctgggacccc caggaggtga 481 ccctgcagct cttcacagat ggaatcacaa ataaacttat tggctgttac gtgggaaaca 541 ccatggagga tgtagtcctg gtgagaattt atggcaataa gactgagtta ttagtcgatc 601 gagatgagga agtaaagagt tttcgagtgt tgcaggctca tgggtgtgca ccacaactct 661 actgtacctt caataatgga ctatgctatg aatttataca aggagaagca ctggatccaa 721 agcatgtctg caacccagcc attttcaggc taatagctcg tcagcttgct aaaatccatg 781 ctattcatgc acacaatggc tggatcccca aatctaatct ttggctaaag atgggaaagt 841 atttctctct cattcccaca ggatttgcag atgaagacat taataaaagg ttcctaagtg 901 atatcccaag ctctcagatt ctccaggaag agatgacttg gatgaaggag attctttcca 961 acctgggctc acctgttgtg ctttgccata atgaccTATT GTGTAAGAAT ATAATCTACA 1021 ATGAGAAACA AGGTGATGTA CAGTTCATTG ATTATGAATA TTCTGGATAC AACTACCTGG 1081 CATATGATAT TGGAAATCAT TTCAATGAAT TTGCAGGTGT GAGTGATGTA GACTATAGTC 1141 TGTATCCAGA TAGAGAACTA CAGAGTCAGT GGCTGCGTGC TTACCTTGAA GCCTACAAAG 1201 AATTTAAGGG CTTTGGGACT GAAGTTACTG AAAAGGAGGT AGAAATACTC TTCATTCAAG 1261 TCAATCAGTT TGCATTGGCT TCTCATTTCT TTTGGGGATT GTGGGCTTTG ATTCAAGCCA 1321 AATACTCCAC TATTGAGTTT GATTTCCTTG GGTATGCAAT TGTTCGTTTT AACCAGTACT 1381 TTAAAATGAA GCCTGAGGTT ACTGCATTAA AAGTGCCTGA GTACCCAACT TTCTTGTACA 1441 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1501 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1561 AGGACGACGA CTAAGAACAG AGCAATGAGG GACGCGTTAA GTCgacaatc aacctctgga 1621 ttacaaaatt tgtgaaagat t