Construct: ORF TRCN0000467065

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004328.1_s317c1
Derived from:
ccsbBroadEn_04817
DNA Barcode:
CAGCGACGATACGTCTCCGCGCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GABPB2 (126626)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467065
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 126626 GABPB2 GA binding protein transcri... NM_001323908.2 100% 100%
2 human 126626 GABPB2 GA binding protein transcri... NM_144618.3 100% 100%
3 human 126626 GABPB2 GA binding protein transcri... XM_017000249.2 100% 100%
4 human 126626 GABPB2 GA binding protein transcri... NM_001323910.2 96.5% 96.5% 108_155del
5 human 126626 GABPB2 GA binding protein transcri... XM_017000246.2 96.5% 96.5% 108_155del
6 human 126626 GABPB2 GA binding protein transcri... XM_017000247.2 96.5% 96.5% 108_155del
7 human 126626 GABPB2 GA binding protein transcri... NM_001323906.2 92.7% 91.2% (many diffs)
8 human 126626 GABPB2 GA binding protein transcri... NM_001323909.2 91.5% 91.5% 619_620ins114
9 human 126626 GABPB2 GA binding protein transcri... XM_017000250.2 88.3% 88.3% 108_155del;667_668ins114
10 human 126626 GABPB2 GA binding protein transcri... NM_001323913.2 87.5% 87.5% 106_107ins168
11 human 126626 GABPB2 GA binding protein transcri... XM_017000252.2 87.5% 87.5% 106_107ins168
12 human 126626 GABPB2 GA binding protein transcri... NM_001323911.2 84.5% 82.8% (many diffs)
13 human 126626 GABPB2 GA binding protein transcri... NM_001323907.2 81.9% 79.9% (many diffs)
14 human 126626 GABPB2 GA binding protein transcri... NM_001323912.2 79% 79% 106_107ins168;451_452ins114
15 human 126626 GABPB2 GA binding protein transcri... XM_017000253.2 66.5% 66.1% (many diffs)
16 human 126626 GABPB2 GA binding protein transcri... XM_017000254.2 60.4% 60% (many diffs)
17 mouse 213054 Gabpb2 GA repeat binding protein, ... NM_029885.1 78.8% 77.4% (many diffs)
18 mouse 213054 Gabpb2 GA repeat binding protein, ... NM_172512.2 78.8% 77.4% (many diffs)
19 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_006501250.3 78.8% 77.4% (many diffs)
20 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_006501251.3 78.8% 77.4% (many diffs)
21 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_006501252.3 78.8% 77.4% (many diffs)
22 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_011240058.2 78.8% 77.4% (many diffs)
23 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_011240059.2 78.8% 77.4% (many diffs)
24 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_011240060.2 78.8% 77.4% (many diffs)
25 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_011240061.2 78.8% 77.4% (many diffs)
26 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_017319515.1 66.4% 65.9% (many diffs)
27 mouse 213054 Gabpb2 GA repeat binding protein, ... XM_006501253.3 58.7% 55.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1410
ORF length:
1344
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tttggtggac ttgggaaaga ggttgctaga agcagcaaga aaaggccaag
121atgatgaagt gagaacgttg atggcaaatg gcgccccatt caccacagac tggcttggaa
181catcacccct ccaccttgca gctcaatatg gtcattattc cacagcagaa gtactccttc
241gagcaggtgt tagcagggat gcccggacta aagtagacag gacccccttg cacatggctg
301cagccgatgg acatgcgcac atcgtggaac tgcttgttcg gaatggtgca gatgtgaatg
361ccaaggacat gctgaagatg acagctttgc attgggccac agagcgccac catcgagatg
421tcgtagagtt acttatcaaa tatggagctg atgtccatgc tttcagcaaa tttgataaat
481cagcctttga catagctctg gagaaaaaca atgctgagat tttggtcatc ctccaggaag
541caatgcagaa tcaggtgaat gttaatccag agagagccaa ccctgtgact gaccctgtga
601gtatggctgc tccattcatc ttcacgtcgg gtgaggttgt taacctcgca agccttattt
661cttcaaccaa caccaaaaca acctcaggtg acccccatgc ctcaacagta cagttttcaa
721attctaccac ctcagtgctg gctacccttg cagctcttgc tgaggcatca gtccccctct
781ccaactcaca cagagccaca gccaatacag aggaaattat agaaggaaat tccgttgact
841catcaatcca gcaagtaatg gggagtggag gccagagggt catcaccata gtgactgatg
901gagtccctct gggtaatatc caaacttcaa tccctactgg aggcattggc cagccattta
961ttgtaactgt gcaagatgga cagcaagttc taactgtacc tgctgGTAAG GTTGCAGAGG
1021AGACTGTAAT TAAAGAGGAA GAAGAAGAGA AGTTGCCACT AACAAAGAAA CCAAGGATAG
1081GAGAGAAGAC AAACAGTGTG GAGGAAAGCA AGGAAGGCAA TGAAAGAGAG CTACTACAGC
1141AACAACTCCA GGAGGCCAAT CGAAGAGCCC AGGAATACCG ACACCAGCTC CTAAAGAAAG
1201AGCAGGAAGC AGAACAGTAC CGTCTTAAGC TGGAGGCCAT AGCCCGACAG CAGCCCAATG
1261GAGTTGATTT CACCATGGTT GAAGAGGTGG CTGAGGTAGA TGCTGTAGTA GTCACAGAGG
1321GGGAGTTGGA AGAGAGAGAG ACAAAAGTGA CTGGGTCAGC AGGGACCACA GAGCCTCACA
1381CTAGAGTTTC CATGGCAACT GTTTCATCTT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1441TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1501CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGCG
1561ACGATACGTC TCCGCGCAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1621tgaaagatt

Download FASTA (ORF) (Full)