Construct: ORF TRCN0000467145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000777.3_s317c1
- Derived from:
- ccsbBroadEn_15119
- DNA Barcode:
- AATAATGTTTAACCGTTACCCGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCK1 (57143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467145
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_020421.4 | 100% | 100% | |
| 2 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366485.2 | 92.5% | 92.5% | 739_740ins117 |
| 3 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366487.2 | 90.1% | 90.1% | 856_1026del |
| 4 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366489.2 | 89.8% | 89.8% | 419_420ins159 |
| 5 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366486.2 | 87.3% | 87.3% | 1006_1007ins198 |
| 6 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001142545.2 | 86.9% | 86.9% | 217_218ins204 |
| 7 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366488.2 | 79.9% | 79.9% | 739_740ins117;889_890ins198 |
| 8 | human | 57143 | ADCK1 | aarF domain containing kina... | NM_001366490.2 | 75.9% | 75.9% | 0_1ins378 |
| 9 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_017021476.1 | 75.9% | 75.9% | 0_1ins378 |
| 10 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_017021477.1 | 75.9% | 75.9% | 0_1ins378 |
| 11 | human | 57143 | ADCK1 | aarF domain containing kina... | XR_001750450.1 | 56% | 1_118del;974_1523del;2238_2801del | |
| 12 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_011536985.1 | 54.4% | 52.3% | (many diffs) |
| 13 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_011536987.2 | 50.5% | 46.3% | (many diffs) |
| 14 | human | 57143 | ADCK1 | aarF domain containing kina... | XM_011536988.2 | 50.5% | 46.3% | (many diffs) |
| 15 | human | 57143 | ADCK1 | aarF domain containing kina... | NR_158988.2 | 46.8% | 1_73del;930_1009del;1723_3348del | |
| 16 | human | 57143 | ADCK1 | aarF domain containing kina... | NR_158989.2 | 42% | 1_73del;929_1387del;2102_3727del | |
| 17 | human | 57143 | ADCK1 | aarF domain containing kina... | NR_158987.2 | 36.7% | (many diffs) | |
| 18 | mouse | 72113 | Adck1 | aarF domain containing kina... | NM_001277296.1 | 85.4% | 88% | (many diffs) |
| 19 | mouse | 72113 | Adck1 | aarF domain containing kina... | NM_001277297.1 | 85.4% | 88% | (many diffs) |
| 20 | mouse | 72113 | Adck1 | aarF domain containing kina... | NM_028105.4 | 85.4% | 88% | (many diffs) |
| 21 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516245.3 | 77.9% | 80.1% | (many diffs) |
| 22 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516246.2 | 73.7% | 75.4% | (many diffs) |
| 23 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516251.2 | 63.5% | 65.5% | (many diffs) |
| 24 | mouse | 72113 | Adck1 | aarF domain containing kina... | XM_006516252.3 | 63.5% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1635
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagaaaggct ctcaagcttg cttcgtggac cagcatggct cttgctgcct 121 ctggcatcta cttctacagt aacaagtact tggaccctaa tgactttggc gctgtcaggg 181 tgggcagagc agttgctacg acggctgtca tcagttacga ctacctcact tccctgaaga 241 gtgtccctta tggctcagag gagtacttgc agctgagatc taaggtgcac cttcgctctg 301 ccaggcgtct ctgtgagctc tgctgtgcca accggggcac cttcatcaag gtgggccagc 361 acctgggggc tctggactac ctgttgccag aggagtacac cagcacgctg aaggtactgc 421 acagccaggc tccacagagc agcatgcaag agatccgcca ggtcatccga gaagatctgg 481 gcaaggagat ccatgatttg ttccagagct tcgatgacac ccctctgggg acggcctccc 541 tggcccaggt ccacaaggca gtgctgcatg atgggcggac ggtggccgtg aaggtccagc 601 acccaaaggt gcgggctcag agctcgaagg acattctcct gatggaggtg ctcgttctgg 661 ctgtgaagca gctgttccca gagtttgagt ttatgtggct tgtggatgaa gccaagaaga 721 acctgccttt ggagctggat ttcctcaatg aagggaggaa tgctgagaag gtgtcccaga 781 tgctcaggca ttttgacttc ttgaaggtcc cccgaatcca ctgggacctg tccacggagc 841 gggtcctcct gatggagttt gtggatggcg ggcaggtcaa tgacagagac tacatggaga 901 ggaacaagat cgacgtcaat gagatctcac gccacctggg caagatgtat agtgagatga 961 tcttcgtcaa tggcttcgtg cactgcgatc cccaccccgg caatgtactg gtgcggaagc 1021 accccggcac gggaaaggcg gagattgtcc tgttggacca tgggctttac cagatgctca 1081 cggaagaatt ccgcctgaat tactgccacc tctggcagtc tctgatctgg actgacatga 1141 agagagtgaa ggagtacagc cagcgactgg gagccgggga tctctacccc ttgtttgcct 1201 gcatgctgac ggcgcgatcg tgggactcgg tcaacagagg catcagccaa gctcccgtca 1261 ctgccactga ggacttagag attcgcaaca acgcggccaa ctaccTCCCC CAGATCAGCC 1321 ATCTCCTCAA CCACGTGCCG CGCCAGATGC TGCTCATCTT GAAGACCAAC GACCTGCTGC 1381 GTGGCATTGA GGCCGCCCTG GGCACCCGCG CCAGCGCCAG CTCCTTTCTC AACATGTCAC 1441 GTTGCTGCAT CAGAGCGCTA GCTGAGCACA AGAAGAAGAA TACCTGTTCA TTCTTCAGAA 1501 GGACCCAGAT CTCTTTCAGC GAGGCCTTCA ACTTATGGCA GATCAACCTC CATGAGCTCA 1561 TCCTGCGTGT GAAGGGGTTG AAGCTGGCTG ACCGGGTCTT GGCCCTAATA TGCTGGCTGT 1621 TCCCTGCTCC ACTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATAATGTTT AACCGTTACC 1801 CGGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt