Construct: ORF TRCN0000467182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011857.1_s317c1
- Derived from:
- ccsbBroadEn_09614
- DNA Barcode:
- AGCTGAGAAGCTCAGATATCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXL14 (144699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144699 | FBXL14 | F-box and leucine rich repe... | NM_152441.3 | 99.9% | 100% | 688C>T |
| 2 | human | 144699 | FBXL14 | F-box and leucine rich repe... | XM_017018875.2 | 96.5% | 96.6% | 688C>T;1149_1150ins42 |
| 3 | human | 144699 | FBXL14 | F-box and leucine rich repe... | XM_017018876.2 | 91.9% | 92.1% | (many diffs) |
| 4 | human | 144699 | FBXL14 | F-box and leucine rich repe... | XR_001748600.2 | 14.5% | (many diffs) | |
| 5 | mouse | 101358 | Fbxl14 | F-box and leucine-rich repe... | NM_133940.3 | 90% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacccacatc tcatgcctgt tcccggagct gctggccatg atcttcggct 121 acctggacgt ccgggacaag gggcgcgcgg cgcaggtgtg caccgcctgg cgggacgccg 181 cctaccacaa gtcggtgtgg cggggggtgg aggccaagct gcacctgcgc cgggccaacc 241 cgtcgctgtt ccccagcctg caggcccggg gcatccgccg ggtgcagatc ctgagcctcc 301 gccgcagcct cagctacgtg atccagggca tggccaacat cgagagcctc aacctcagcg 361 gctgctacaa cctcaccgac aacgggctgg gccacgcgtt tgtgcaggag atcggctccc 421 tgcgcgctct caacctgagc ctctgcaagc agatcactga cagcagcctg ggccgcatag 481 cccagtacct caagggcctg gaggtgctgg agctgggagg ttgcagcaac atcaccaaca 541 ctggccttct gctcatcgcc tggggtctgc agcgcctcaa gagccttaac ctccgcagct 601 gccgccacct ttcggatgtg ggcatcgggc acctggccgg catgacgcgc agcgcggcgg 661 agggctgcct gggcctggag cagctcacgc tacaggactg ccagaagctc acagatcttt 721 ctctaaagca catctcccga gggctgacgg gcttgaggct cctcaacctc agcttctgtg 781 ggggaatctc ggacgctggc ctcctgcacc tgtcgcacat gggcagcctg cgcagcctca 841 acctgcgctc ctgtgacaac atcagtgaca cgggcatcat gcatctggcc atgggcagcc 901 tgcgcctctc ggggctggat gtttcgttct gtgacaaggt gggagaccag agtctggctt 961 acatagccca ggggctggat ggcctcaagt ctctctccct ctgctcctgc cacatcagtg 1021 atgatggcat caaccgcatg gTGCGGCAGA TGCACGGGCT GCGCACGCTC AACATTGGAC 1081 AGTGTGTGCG CATCACGGAC AAGGGCCTGG AGCTGATCGC TGAGCACCTG AGCCAACTCA 1141 CCGGCATAGA CCTGTACGGC TGCACCCGAA TCACCAAGCG CGGCCTGGAG CGCATCACGC 1201 AGCTGCCGTG CCTCAAGGTA CTCAACCTGG GACTCTGGCA GATGACGGAC AGTGAGAAGG 1261 AGGCACGAGG GGATTTTTCT CCATTATTCA CTGTGAGAAC TCGGGGAAGC TCCAGAAGGT 1321 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAAGCTG AGAAGCTCAG ATATCAATTA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt