Construct: ORF TRCN0000467367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009364.1_s317c1
- Derived from:
- ccsbBroadEn_11020
- DNA Barcode:
- ACTCGGGGTTTTGCCCGAAGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCM1 (5108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467367
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352659.1 | 100% | 100% | |
| 2 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352660.1 | 100% | 100% | |
| 3 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352643.2 | 34.9% | 34.9% | 476_477delACinsGT;1591_4545del |
| 4 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352654.2 | 33.5% | 33.5% | 476_477delACinsGT;783_784ins117;1474_4263del |
| 5 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352655.2 | 33.5% | 33.5% | 476_477delACinsGT;783_784ins117;1474_4263del |
| 6 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352656.2 | 33.5% | 33.5% | 476_477delACinsGT;783_784ins117;1474_4263del |
| 7 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352657.2 | 32.3% | 32.3% | 476_477delACinsGT;783_784ins117;1474_4428del |
| 8 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352658.2 | 32.3% | 32.3% | 476_477delACinsGT;783_784ins117;1474_4428del |
| 9 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352638.2 | 26.4% | 26.4% | 476_477delACinsGT;1591_6000del |
| 10 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013490.2 | 26.4% | 26.4% | 476_477delACinsGT;1591_6000del |
| 11 | human | 5108 | PCM1 | pericentriolar material 1 | XM_024447171.1 | 26.4% | 26.4% | 476_477delACinsGT;1591_6000del |
| 12 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352637.2 | 26.3% | 26.3% | 476_477delACinsGT;1591_6024del |
| 13 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013489.1 | 26.3% | 26.3% | 476_477delACinsGT;1591_6024del |
| 14 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352636.2 | 26.3% | 26.3% | 476_477delACinsGT;1591_6027del |
| 15 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013486.1 | 26.3% | 26.2% | 476_477delACinsGT;1591_6036del |
| 16 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001315508.1 | 25.8% | 25.8% | 783_784ins117;1474_5580del |
| 17 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352642.2 | 25.8% | 25.8% | 476_477delACinsGT;783_784ins117;1474_5577del |
| 18 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013478.1 | 25.7% | 25.7% | 476_477delACinsGT;1591_6165del |
| 19 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352633.2 | 25.6% | 25.6% | 476_477delACinsGT;1591_6186del |
| 20 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352650.2 | 25.6% | 25.6% | 476_477delACinsGT;1591_6189del |
| 21 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352651.2 | 25.6% | 25.6% | 476_477delACinsGT;1591_6189del |
| 22 | human | 5108 | PCM1 | pericentriolar material 1 | XM_024447168.1 | 25.6% | 25.6% | 476_477delACinsGT;1591_6189del |
| 23 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352632.2 | 25.6% | 25.5% | 476_477delACinsGT;1591_6201del |
| 24 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013473.1 | 25.2% | 25.2% | 476_477delACinsGT;1591_6279del |
| 25 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013472.1 | 25.2% | 25.2% | 476_477delACinsGT;1591_6282del |
| 26 | human | 5108 | PCM1 | pericentriolar material 1 | XM_006716340.3 | 24.6% | 24.6% | 476_477delACinsGT;900_1088del;1780_6438del |
| 27 | human | 5108 | PCM1 | pericentriolar material 1 | XM_011544534.2 | 24.6% | 24.6% | 476_477delACinsGT;900_1088del;1780_6447del |
| 28 | human | 5108 | PCM1 | pericentriolar material 1 | XM_006716336.3 | 24.6% | 24.6% | 476_477delACinsGT;900_1088del;1780_6450del |
| 29 | human | 5108 | PCM1 | pericentriolar material 1 | XM_011544532.2 | 24.5% | 24.5% | 476_477delACinsGT;900_1088del;1780_6468del |
| 30 | human | 5108 | PCM1 | pericentriolar material 1 | XM_011544528.2 | 24.5% | 24.5% | 476_477delACinsGT;900_1088del;1780_6471del |
| 31 | human | 5108 | PCM1 | pericentriolar material 1 | XM_011544529.2 | 24.5% | 24.5% | 476_477delACinsGT;900_1088del;1780_6471del |
| 32 | human | 5108 | PCM1 | pericentriolar material 1 | XM_011544530.2 | 24.5% | 24.5% | 476_477delACinsGT;900_1088del;1780_6471del |
| 33 | human | 5108 | PCM1 | pericentriolar material 1 | XM_024447167.1 | 24.5% | 24.5% | 476_477delACinsGT;900_1088del;1780_6471del |
| 34 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352646.2 | 24.5% | 24.5% | 476_477delACinsGT;783_784ins117;1474_5883del |
| 35 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352647.2 | 24.5% | 24.5% | 476_477delACinsGT;783_784ins117;1474_5883del |
| 36 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013501.1 | 24.5% | 24.5% | 476_477delACinsGT;783_784ins117;1474_5883del |
| 37 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352648.2 | 24.5% | 24.4% | 476_477delACinsGT;783_784ins117;1474_5886del |
| 38 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352649.2 | 24.5% | 24.4% | 476_477delACinsGT;783_784ins117;1474_5886del |
| 39 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352641.2 | 24.4% | 24.4% | 476_477delACinsGT;783_784ins117;1474_5895del |
| 40 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352644.2 | 24.4% | 24.4% | 476_477delACinsGT;783_784ins117;1474_5907del |
| 41 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352645.2 | 24.4% | 24.4% | 476_477delACinsGT;783_784ins117;1474_5907del |
| 42 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013496.2 | 24.4% | 24.4% | 476_477delACinsGT;783_784ins117;1474_5907del |
| 43 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352640.2 | 24.4% | 24.3% | 476_477delACinsGT;783_784ins117;1474_5910del |
| 44 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352639.2 | 24.3% | 24.3% | 476_477delACinsGT;783_784ins117;1474_5919del |
| 45 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013493.1 | 24.3% | 24.3% | 476_477delACinsGT;783_784ins117;1474_5919del |
| 46 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013491.1 | 24.3% | 24.3% | 476_477delACinsGT;783_784ins117;1474_5922del |
| 47 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001315507.2 | 23.8% | 23.8% | 476_477delACinsGT;783_784ins117;1474_6048del |
| 48 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352635.2 | 23.8% | 23.8% | 476_477delACinsGT;783_784ins117;1474_6051del |
| 49 | human | 5108 | PCM1 | pericentriolar material 1 | XM_024447169.1 | 23.8% | 23.8% | 476_477delACinsGT;783_784ins117;1474_6051del |
| 50 | human | 5108 | PCM1 | pericentriolar material 1 | XM_024447170.1 | 23.8% | 23.8% | 476_477delACinsGT;783_784ins117;1474_6051del |
| 51 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352652.2 | 23.7% | 23.7% | 476_477delACinsGT;783_784ins117;1474_6072del |
| 52 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352653.2 | 23.7% | 23.7% | 476_477delACinsGT;783_784ins117;1474_6072del |
| 53 | human | 5108 | PCM1 | pericentriolar material 1 | NM_006197.4 | 23.7% | 23.7% | 476_477delACinsGT;783_784ins117;1474_6072del |
| 54 | human | 5108 | PCM1 | pericentriolar material 1 | NM_001352634.2 | 23.7% | 23.7% | 476_477delACinsGT;783_784ins117;1474_6084del |
| 55 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013480.1 | 23.5% | 23.4% | 476_477delACinsGT;783_784ins117;1474_6141del |
| 56 | human | 5108 | PCM1 | pericentriolar material 1 | XM_017013481.1 | 23.5% | 23.4% | 476_477delACinsGT;783_784ins117;1474_6141del |
| 57 | human | 5108 | PCM1 | pericentriolar material 1 | NR_148032.2 | 16.8% | (many diffs) | |
| 58 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_006509304.2 | 29.7% | 29.9% | (many diffs) |
| 59 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_011242179.1 | 29.7% | 29.9% | (many diffs) |
| 60 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_006509302.2 | 23.8% | 24% | (many diffs) |
| 61 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_006509301.2 | 23.8% | 24% | (many diffs) |
| 62 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_006509300.2 | 23.7% | 23.9% | (many diffs) |
| 63 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_006509298.2 | 23.2% | 23.4% | (many diffs) |
| 64 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_017312615.1 | 21.9% | 22.2% | (many diffs) |
| 65 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XM_006509299.2 | 21.6% | 21.8% | (many diffs) |
| 66 | mouse | 18536 | Pcm1 | pericentriolar material 1 | NM_023662.3 | 21.4% | 21.6% | (many diffs) |
| 67 | mouse | 18536 | Pcm1 | pericentriolar material 1 | XR_001778411.1 | 16.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacaggagga ggtccctttg aagatggcat gaatgatcag gatttaccaa 121 actggagtaa tgagaatgtt gatgacaggc tcaacaatat ggattggggt gcccaacaga 181 agaaagcaaa tagatcatca gaaaagaata agaaaaagtt tggtgtagaa agtgataaaa 241 gagtaaccaa tgatatttct ccggagtcgt caccaggagt tggaaggcga agaacaaaga 301 ctccacatac gttcccacac agtagataca tgagtcagat gtctgtccca gagcaggcag 361 aattagagaa actgaaacag cggataaact tcagtgattt agatcagaga agcattggaa 421 gtgattccca aggtagagca acagctgcta acaacaaacg tcagcttagt gaaaaccgaa 481 agcccttcaa ctttttgcct atgcagatta atactaacaa gagcaaagat gcatctacaa 541 gtcccccaaa cagagaaacg attggatcag cacagtgtaa agagttgttt gcttctgctt 601 taagtaatga cctcttgcaa aactgtcagg tgtctgaaga agatgggagg ggagaacctg 661 caatggagag cagccagatt gtaagcaggc ttgttcaaat tcgcgattat attactaaag 721 ctagttccat gcgggaagat cttgtagaga aaaatgagag atctgctaat gttgagcgcc 781 ttactcatct aatagatcac cttaaagaac aagagaagtc atatatgaaa tttcttaaaa 841 aaatccttgc tagagaaaat gaggaggagg atgttcggac tatagattca gctgtgggat 901 ctggttctgt agctgagagc acatcgctaa acatagatgt gcagtctgag gcttcagata 961 ccacggccag agatcctcag caggagccta tggaagagat agaaaatttg aagaaacaac 1021 atgatttatt aaaaagaatg ttacaacagc aggagcaact aagagctcta cagggacggc 1081 aggctgcact tctagctctg caacataaag cagagcaagc tattgcagtg atggatgatt 1141 ctgttgttgc agaaactgca ggtagcttat ctggcgtcag tatcacatct gaactaaatg 1201 aagaattgaa tgacttaatt cagcgttttc ataatcagct tcgtgattct cagcctccag 1261 ctgttccaga caatagaaga caggcagaaa gtctttcatt aactagggag gtttcccaga 1321 gcaggaaacc atcagcttca gaacgtttac cTGATGAGAA AGTCGAACTT TTTAGCAAAA 1381 TGAGAGTGCT ACAGGAAAAG AAACAAAAAA TGGACAAATT GCTTGGAGAA CTTCATACAC 1441 TTCGAGATCA GCATCTTAAC AATTCATCAT CCTCTCCACA AAGGAGTGTC GATCAGAGAA 1501 GTACTTCAGC TCCCTCTGCT TCTGTAGGCT TGGCACCGGT TGTCAATGGA GAATCCAATA 1561 GCCTCACATC ATCTGTTCCT TATCCTACTG CTTCTCTAGT ATCTCAGAAT GAGAGTGAAA 1621 ACGAAGGCCA CCTCAATCCA TCTGAAAAAC TCCAGTACCC AACTTTCTTG TACAAAGTGG 1681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1801 AACTCGGGGT TTTGCCCGAA GCCTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1861 aatttgtgaa agatt