Construct: ORF TRCN0000467427
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000034.1_s317c1
- Derived from:
- ccsbBroadEn_00978
- DNA Barcode:
- CCTTCTTCGGGGACGGAAGTTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAX (4149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467427
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4149 | MAX | MYC associated factor X | NM_002382.5 | 100% | 100% | |
| 2 | human | 4149 | MAX | MYC associated factor X | NM_145112.3 | 94.3% | 94.3% | 36_37ins27 |
| 3 | human | 4149 | MAX | MYC associated factor X | XM_011536773.3 | 74.7% | 61% | (many diffs) |
| 4 | human | 4149 | MAX | MYC associated factor X | NM_145113.3 | 63.5% | 61.2% | (many diffs) |
| 5 | human | 4149 | MAX | MYC associated factor X | NM_001320415.2 | 52% | 38.3% | (many diffs) |
| 6 | human | 4149 | MAX | MYC associated factor X | XM_017021312.2 | 52% | 38.3% | (many diffs) |
| 7 | human | 4149 | MAX | MYC associated factor X | XM_017021313.1 | 52% | 38.3% | (many diffs) |
| 8 | human | 4149 | MAX | MYC associated factor X | NM_197957.3 | 47.6% | 36.1% | (many diffs) |
| 9 | human | 4149 | MAX | MYC associated factor X | NM_145114.2 | 45.9% | 37.9% | (many diffs) |
| 10 | human | 4149 | MAX | MYC associated factor X | NM_001271069.1 | 42% | 30.6% | (many diffs) |
| 11 | human | 4149 | MAX | MYC associated factor X | NM_001271068.1 | 40.7% | 32.5% | (many diffs) |
| 12 | human | 4149 | MAX | MYC associated factor X | NR_073137.1 | 23.2% | (many diffs) | |
| 13 | human | 4149 | MAX | MYC associated factor X | XR_943450.3 | 21.8% | 1_202del;498_663del;849_2201del | |
| 14 | human | 4149 | MAX | MYC associated factor X | XR_943451.3 | 21.6% | 1_202del;497_678del;865_2217del | |
| 15 | human | 4149 | MAX | MYC associated factor X | XR_001750327.2 | 21.3% | (many diffs) | |
| 16 | human | 4149 | MAX | MYC associated factor X | XR_943452.3 | 20.6% | (many diffs) | |
| 17 | human | 4149 | MAX | MYC associated factor X | XR_001750326.2 | 20.5% | (many diffs) | |
| 18 | human | 4149 | MAX | MYC associated factor X | XR_002957553.1 | 18.3% | 1_193del;488_1072del;1259_2611del | |
| 19 | mouse | 17187 | Max | Max protein | NM_008558.2 | 96% | 99.3% | (many diffs) |
| 20 | mouse | 17187 | Max | Max protein | NM_001146176.1 | 90.4% | 93.7% | (many diffs) |
| 21 | mouse | 17187 | Max | Max protein | XM_006515523.3 | 73.9% | 76.8% | (many diffs) |
| 22 | mouse | 17187 | Max | Max protein | XM_006515524.3 | 61.6% | 61.2% | (many diffs) |
| 23 | mouse | 17187 | Max | Max protein | XM_017314968.1 | 37.5% | 38.9% | (many diffs) |
| 24 | mouse | 17187 | Max | Max protein | XR_381483.3 | 21.1% | (many diffs) | |
| 25 | mouse | 17187 | Max | Max protein | XR_872708.2 | 20.9% | (many diffs) | |
| 26 | mouse | 17187 | Max | Max protein | XR_001780418.1 | 20.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgataacgat gacatcgagg tggagagcga cgaagagcaa ccgaggtttc 121 aatctgcggc tgacaaacgg gctcatcata atgcactgga acgaaaacgt agggaccaca 181 tcaaagacag ctttcacagt ttgcgggacT CAGTCCCATC ACTCCAAGGA GAGAAGGCAT 241 CCCGGGCCCA AATCCTAGAC AAAGCCACAG AATATATCCA GTATATGCGA AGGAAAAACC 301 ACACACACCA GCAAGATATT GACGACCTCA AGCGGCAGAA TGCTCTTCTG GAGCAGCAAG 361 TCCGTGCACT GGAGAAGGCG AGGTCAAGTG CCCAACTGCA GACCAACTAC CCCTCCTCAG 421 ACAACAGCCT CTACACCAAC GCCAAGGGCA GCACCATCTC TGCCTTCGAT GGGGGCTCGG 481 ACTCCAGCTC GGAGTCTGAG CCTGAAGAGC CCCAAAGCAG GAAGAAGCTC CGGATGGAGG 541 CCAGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTTCTTCG GGGACGGAAG TTACAACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt