Construct: ORF TRCN0000467428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012849.1_s317c1
- Derived from:
- ccsbBroadEn_02555
- DNA Barcode:
- ACCCGCCTCTGCCGCGGTAATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TXNL4A (10907)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10907 | TXNL4A | thioredoxin like 4A | NM_006701.5 | 100% | 100% | |
| 2 | human | 10907 | TXNL4A | thioredoxin like 4A | NM_001305557.2 | 94.3% | 94.3% | 127_128ins24 |
| 3 | human | 10907 | TXNL4A | thioredoxin like 4A | NM_001303471.3 | 69.5% | 62.8% | (many diffs) |
| 4 | human | 10907 | TXNL4A | thioredoxin like 4A | NM_001305563.2 | 50% | 50% | 0_1ins213 |
| 5 | human | 10907 | TXNL4A | thioredoxin like 4A | NM_001305564.2 | 50% | 50% | 0_1ins213 |
| 6 | human | 10907 | TXNL4A | thioredoxin like 4A | NR_131176.2 | 12% | 1_171del;428_497del;668_3542del | |
| 7 | human | 10907 | TXNL4A | thioredoxin like 4A | NR_131175.2 | 11.5% | (many diffs) | |
| 8 | human | 10907 | TXNL4A | thioredoxin like 4A | NR_131177.2 | 9.2% | 1_171del;322_323ins104;494_3368del | |
| 9 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | NM_025299.3 | 89.9% | 100% | (many diffs) |
| 10 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | XM_006526487.3 | 89.9% | 100% | (many diffs) |
| 11 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | XR_386372.1 | 46.4% | (many diffs) | |
| 12 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | XM_017317914.1 | 43.1% | 50% | (many diffs) |
| 13 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | NM_001038608.2 | 38.7% | 36.3% | (many diffs) |
| 14 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | NM_001042408.1 | 38.7% | 36.3% | (many diffs) |
| 15 | mouse | 27366 | Txnl4a | thioredoxin-like 4A | NM_178604.3 | 38.7% | 36.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtacatgctc ccgcacctgc acaacggctg gcaggtggac caggccatcc 121 tctcggagga ggaccgcgtg gtcgtcatcc gcttcggcca cgactgggat ccTACGTGCA 181 TGAAGATGGA CGAGGTCCTG TACAGCATCG CCGAGAAGGT TAAAAATTTT GCAGTTATTT 241 ATCTTGTGGA TATTACAGAA GTGCCTGACT TCAACAAAAT GTATGAGTTA TACGATCCAT 301 GTACTGTCAT GTTTTTCTTC AGGAACAAGC ACATCATGAT TGACTTGGGG ACTGGCAACA 361 ACAACAAGAT TAACTGGGCC ATGGAGGACA AGCAGGAGAT GGTGGACATC ATCGAGACGG 421 TGTACCGCGG GGCCCGCAAA GGCCGCGGCC TGGTGGTGTC CCCCAAGGAC TACTCCACCA 481 AGTACCGCTA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACC CGCCTCTGCC GCGGTAATAC 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t