Construct: ORF TRCN0000467491
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015444.1_s317c1
- Derived from:
- ccsbBroadEn_13719
- DNA Barcode:
- ATCAGATGCCTACGAAGGCAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC642204 (642204)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467491
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XM_011543861.3 | 29.6% | 17% | (many diffs) |
| 2 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_011517879.2 | 29.5% | 17% | (many diffs) |
| 3 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XM_005272525.4 | 16.1% | 9.4% | (many diffs) |
| 4 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | NM_001012419.2 | 16% | 9.4% | (many diffs) |
| 5 | human | 84210 | ANKRD20A1 | ankyrin repeat domain 20 fa... | NM_032250.3 | 16% | 9.4% | (many diffs) |
| 6 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_024447553.1 | 16% | 9.4% | (many diffs) |
| 7 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | NM_001012421.1 | 16% | 9.4% | (many diffs) |
| 8 | human | 728747 | ANKRD20A4 | ankyrin repeat domain 20 fa... | NM_001098805.1 | 16% | 9.2% | (many diffs) |
| 9 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XM_011543860.3 | 15.8% | 9.2% | (many diffs) |
| 10 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_011517878.2 | 15.8% | 9.2% | (many diffs) |
| 11 | human | 441430 | ANKRD20A2 | ankyrin repeat domain 20 fa... | XM_011517877.3 | 15.7% | 9.2% | (many diffs) |
| 12 | human | 102723552 | ANKRD20A21P | ankyrin repeat domain 20 fa... | XR_002958558.1 | 13.5% | (many diffs) | |
| 13 | human | 440482 | ANKRD20A5P | ankyrin repeat domain 20 fa... | NR_040113.1 | 10.4% | (many diffs) | |
| 14 | human | 110006327 | ANKRD20A4-ANKRD20A20P | ANKRD20A4-ANKRD20A20P readt... | NR_146419.1 | 9% | (many diffs) | |
| 15 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XR_001746296.2 | 8.5% | (many diffs) | |
| 16 | human | 441425 | ANKRD20A3 | ankyrin repeat domain 20 fa... | XR_001746295.2 | 8.4% | (many diffs) | |
| 17 | human | 729171 | ANKRD20A8P | ankyrin repeat domain 20 fa... | NR_003366.2 | 8% | (many diffs) | |
| 18 | human | 400110 | ANKRD20A19P | ankyrin repeat domain 20 fa... | NR_073430.1 | 6.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 495
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagttattt gccTTCGGGA GCCGCAGGGG CCAGACGGCC CAGGGCTCTA 121 TAGAACATGT CTACACGGGT TCCGGATACC GAATCCGGGA CTCCGAACTG CAGAAGATCC 181 ACAGGGCAGC TGTCAAGGGC GACGCCGCGG GGGTGGAGCG CTGCCTGGCG CGCAGGAGCG 241 GAGACCTGGA CGCCCTGGAC AAGCAGCACA GGCTGTCCAT TGCCAGGAAG AGGCTTGCGC 301 CGTTATTCTG CTGGAACATG GCACCAATCC AAACCTTAAG GATATCTACC GCAACACTGC 361 TCTCCAGTAT GCTGTGTATA GTGAGAGCAC CTCACTGGCA GAAAAACTGC TTTTCCATGG 421 TGCAAATATT GAAGCACTGG ACAAGGTATA GATCAATCAA CTTTCTTTCC AAAATATTTG 481 TTTTAACATT GACATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCAGATGCC TACGAAGGCA 661 GTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt