Transcript: Human NM_001012419.2

Homo sapiens ankyrin repeat domain 20 family member A3 (ANKRD20A3), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
ANKRD20A3 (441425)
Length:
3930
CDS:
79..2550

Additional Resources:

NCBI RefSeq record:
NM_001012419.2
NBCI Gene record:
ANKRD20A3 (441425)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001012419.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000262908 ATTAGGCACAGAGTCATATTT pLKO_005 3002 3UTR 100% 15.000 7.500 Y ANKRD20A4 n/a
2 TRCN0000168760 GCACTGGACAAGGACAATAAT pLKO.1 559 CDS 100% 15.000 7.500 Y ANKRD20A1 n/a
3 TRCN0000167418 GCAGAAGCTAAAGTGAATAAT pLKO.1 2224 CDS 100% 15.000 7.500 Y ANKRD20A1 n/a
4 TRCN0000167796 GCCTCATTTCTAGGAAATAAA pLKO.1 2922 3UTR 100% 15.000 7.500 Y ANKRD20A1 n/a
5 TRCN0000419116 ATATCCAAAGAGGCTTTATTG pLKO_005 2366 CDS 100% 13.200 6.600 Y ANKRD20A1 n/a
6 TRCN0000262907 ATGCAGAAGATTATGCTATTT pLKO_005 779 CDS 100% 13.200 6.600 Y ANKRD20A4 n/a
7 TRCN0000167112 CACAGGTCAAAGAAGGAAATA pLKO.1 1226 CDS 100% 13.200 6.600 Y ANKRD20A1 n/a
8 TRCN0000434616 CATCAGAAAGAGGATCATAAA pLKO_005 2332 CDS 100% 13.200 6.600 Y ANKRD20A1 n/a
9 TRCN0000128130 CGGTATCAACAAGAGCTTAAT pLKO.1 1795 CDS 100% 13.200 6.600 Y ANKRD20A2 n/a
10 TRCN0000157113 GAAGAGGCTTGTGCCGTTATT pLKO.1 412 CDS 100% 13.200 6.600 Y ANKRD20A5P n/a
11 TRCN0000134343 GCATGATAGATGCACCTTAAA pLKO.1 1386 CDS 100% 13.200 6.600 Y ANKRD20A3 n/a
12 TRCN0000128553 GCCACATGTTAAGACCATAAT pLKO.1 3075 3UTR 100% 13.200 6.600 Y ANKRD20A2 n/a
13 TRCN0000129983 GCTTAGAAACATGCCTCATTT pLKO.1 2910 3UTR 100% 13.200 6.600 Y ANKRD20A2 n/a
14 TRCN0000262906 TCATCAGAAAGAGGATCATAA pLKO_005 2331 CDS 100% 13.200 6.600 Y ANKRD20A4 n/a
15 TRCN0000262909 ACTATGACTCACCAGGTATTG pLKO_005 701 CDS 100% 10.800 5.400 Y ANKRD20A4 n/a
16 TRCN0000127485 CCACAGGTCAAAGAAGGAAAT pLKO.1 1225 CDS 100% 10.800 5.400 Y ANKRD20A2 n/a
17 TRCN0000432708 GATGCAGAAGATTATGCTATT pLKO_005 778 CDS 100% 10.800 5.400 Y ANKRD20A1 n/a
18 TRCN0000262905 TGTGAAAGGAGCAGTACAAAG pLKO_005 1113 CDS 100% 10.800 5.400 Y ANKRD20A4 n/a
19 TRCN0000168216 CAAGTTCACATGCCGTTGATA pLKO.1 647 CDS 100% 5.625 2.813 Y ANKRD20A1 n/a
20 TRCN0000155962 CTGTGAAAGGAGCAGTACAAA pLKO.1 1112 CDS 100% 5.625 2.813 Y ANKRD20A11P n/a
21 TRCN0000167295 CAGATTGATGTCTGTGACAAA pLKO.1 352 CDS 100% 4.950 2.475 Y ANKRD20A1 n/a
22 TRCN0000128666 CCAATCCAAACCTTAAGGATA pLKO.1 449 CDS 100% 4.950 2.475 Y ANKRD20A2 n/a
23 TRCN0000133759 CCAATCCAAACCTTAAGGATA pLKO.1 449 CDS 100% 4.950 2.475 Y ANKRD20A3 n/a
24 TRCN0000129747 CCCAGATGAATTGAAGTGTAT pLKO.1 3188 3UTR 100% 4.950 2.475 Y ANKRD20A2 n/a
25 TRCN0000137775 GCAAGGCTCAATGACTGTCAT pLKO.1 3480 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
26 TRCN0000138952 GCCGTTATTCTGCTGGAACAT pLKO.1 424 CDS 100% 4.950 2.475 Y ANKRD20A3 n/a
27 TRCN0000138852 GCTCTCCATTATGCCGTGTAT pLKO.1 484 CDS 100% 4.950 2.475 Y ANKRD20A3 n/a
28 TRCN0000128015 GCTGAGAATACAAGGCTCAAT pLKO.1 1825 CDS 100% 4.950 2.475 Y ANKRD20A2 n/a
29 TRCN0000136726 GCTGAGAATACAAGGCTCAAT pLKO.1 1825 CDS 100% 4.950 2.475 Y ANKRD20A3 n/a
30 TRCN0000172688 GCTGGCAGATTAACCCAACAA pLKO.1 1312 CDS 100% 4.950 2.475 Y ANKRD20A1 n/a
31 TRCN0000156881 GCTGTGAAAGGAGCAGTACAA pLKO.1 1111 CDS 100% 4.950 2.475 Y ANKRD20A11P n/a
32 TRCN0000137143 GCTGTCTGTGATTTACGAGTT pLKO.1 3537 3UTR 100% 4.050 2.025 Y ANKRD20A3 n/a
33 TRCN0000134908 CCAGGTATTGTCAATATCCTT pLKO.1 712 CDS 100% 3.000 1.500 Y ANKRD20A3 n/a
34 TRCN0000153376 GCCAATCCAAACCTTAAGGAT pLKO.1 448 CDS 100% 3.000 1.500 Y ANKRD20A5P n/a
35 TRCN0000157682 CCTTTGATACAGGCTGTCCAT pLKO.1 385 CDS 100% 2.640 1.320 Y ANKRD20A5P n/a
36 TRCN0000131073 GCCAGATTGATGTCTGTGACA pLKO.1 350 CDS 100% 2.640 1.320 Y ANKRD20A2 n/a
37 TRCN0000137846 GTGTGGATTCATTGCCTGCAT pLKO.1 920 CDS 100% 2.640 1.320 Y ANKRD20A3 n/a
38 TRCN0000156507 GTATAGTGAGAGCACCTCACT pLKO.1 501 CDS 100% 0.264 0.132 Y ANKRD20A5P n/a
39 TRCN0000423507 GGTATCAACAAGAGCTTAATG pLKO_005 1796 CDS 100% 13.200 6.600 Y ANKRD20A1 n/a
40 TRCN0000139212 CAGCACAGAACTGCTCTACAT pLKO.1 274 CDS 100% 4.950 2.475 Y ANKRD20A19P n/a
41 TRCN0000158171 CCTTGAAGCCTAGCACTGAAA pLKO.1 1079 CDS 100% 4.950 2.475 Y ANKRD20A11P n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001012419.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13700 pDONR223 100% 99.8% 99.7% None 893_895delAGC;1738T>G n/a
2 ccsbBroad304_13700 pLX_304 0% 99.8% 99.7% V5 893_895delAGC;1738T>G n/a
3 TRCN0000476704 CAATTCCTCTTAAGATTAAATCAT pLX_317 15.4% 99.8% 99.7% V5 893_895delAGC;1738T>G n/a
4 ccsbBroadEn_13693 pDONR223 100% 19% 18.3% None (many diffs) n/a
5 ccsbBroadEn_13719 pDONR223 100% 16% 9.4% None (many diffs) n/a
6 ccsbBroad304_13719 pLX_304 0% 16% 9.4% V5 (many diffs) n/a
7 TRCN0000467491 ATCAGATGCCTACGAAGGCAGTTC pLX_317 93.9% 16% 9.4% V5 (many diffs) n/a
8 ccsbBroadEn_13631 pDONR223 100% 5.9% 4.8% None (many diffs) n/a
9 ccsbBroad304_13631 pLX_304 0% 5.9% 4.8% V5 (many diffs) n/a
10 TRCN0000477773 GCACCGACAGTTTAATGATTATCA pLX_317 100% 5.9% 4.8% V5 (many diffs) n/a
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