Transcript: Human NR_073430.1

Homo sapiens ankyrin repeat domain 20 family member A19, pseudogene (ANKRD20A19P), non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
ANKRD20A19P (400110)
Length:
5096
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_073430.1
NBCI Gene record:
ANKRD20A19P (400110)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_073430.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140155 GCTGATAATTCGGCGATGCTT pLKO.1 4583 3UTR 100% 3.000 4.200 N ANKRD20A19P n/a
2 TRCN0000144322 CCAAATTGACATCTGTGACAA pLKO.1 3636 3UTR 100% 4.950 3.465 N ANKRD20A19P n/a
3 TRCN0000138993 CCTCTTCAGTTGCTGAGACTA pLKO.1 3956 3UTR 100% 4.950 3.465 N ANKRD20A19P n/a
4 TRCN0000139518 CACCAAGAAGTTCTTCGGCTT pLKO.1 3369 3UTR 100% 2.160 1.512 N ANKRD20A19P n/a
5 TRCN0000145562 CCTGTCCAAATCTTTCCAATA pLKO.1 4750 3UTR 100% 10.800 6.480 N ANKRD20A19P n/a
6 TRCN0000139635 CACCACCACCAAGAAGTTCTT pLKO.1 3363 3UTR 100% 4.950 2.970 N ANKRD20A19P n/a
7 TRCN0000121674 GCACAGAACTGCTCTACATTT pLKO.1 3561 3UTR 100% 13.200 6.600 Y ANKRD20A19P n/a
8 TRCN0000139212 CAGCACAGAACTGCTCTACAT pLKO.1 3559 3UTR 100% 4.950 2.475 Y ANKRD20A19P n/a
9 TRCN0000140248 GCTCTCCAGTATGCTGTGTAT pLKO.1 3775 3UTR 100% 4.950 2.475 Y ANKRD20A19P n/a
10 TRCN0000153376 GCCAATCCAAACCTTAAGGAT pLKO.1 3739 3UTR 100% 3.000 1.500 Y ANKRD20A5P n/a
11 TRCN0000140773 GAACTGCTCTACATTTGGCCT pLKO.1 3566 3UTR 100% 0.660 0.330 Y ANKRD20A19P n/a
12 TRCN0000157562 GAACTGCTCTACATTTGGCCT pLKO.1 3566 3UTR 100% 0.660 0.330 Y ANKRD20A5P n/a
13 TRCN0000128666 CCAATCCAAACCTTAAGGATA pLKO.1 3740 3UTR 100% 4.950 2.475 Y ANKRD20A2 n/a
14 TRCN0000133759 CCAATCCAAACCTTAAGGATA pLKO.1 3740 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_073430.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13693 pDONR223 100% 8.4% None (many diffs) n/a
2 ccsbBroadEn_13719 pDONR223 100% 6.9% None (many diffs) n/a
3 ccsbBroad304_13719 pLX_304 0% 6.9% V5 (many diffs) n/a
4 TRCN0000467491 ATCAGATGCCTACGAAGGCAGTTC pLX_317 93.9% 6.9% V5 (many diffs) n/a
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