Transcript: Human NR_003366.2

Homo sapiens ankyrin repeat domain 20 family member A8, pseudogene (ANKRD20A8P), non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
ANKRD20A8P (729171)
Length:
3670
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_003366.2
NBCI Gene record:
ANKRD20A8P (729171)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_003366.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000167418 GCAGAAGCTAAAGTGAATAAT pLKO.1 1963 3UTR 100% 15.000 7.500 Y ANKRD20A1 n/a
2 TRCN0000419116 ATATCCAAAGAGGCTTTATTG pLKO_005 2105 3UTR 100% 13.200 6.600 Y ANKRD20A1 n/a
3 TRCN0000434616 CATCAGAAAGAGGATCATAAA pLKO_005 2071 3UTR 100% 13.200 6.600 Y ANKRD20A1 n/a
4 TRCN0000128130 CGGTATCAACAAGAGCTTAAT pLKO.1 1534 3UTR 100% 13.200 6.600 Y ANKRD20A2 n/a
5 TRCN0000157113 GAAGAGGCTTGTGCCGTTATT pLKO.1 240 3UTR 100% 13.200 6.600 Y ANKRD20A5P n/a
6 TRCN0000423507 GGTATCAACAAGAGCTTAATG pLKO_005 1535 3UTR 100% 13.200 6.600 Y ANKRD20A1 n/a
7 TRCN0000262906 TCATCAGAAAGAGGATCATAA pLKO_005 2070 3UTR 100% 13.200 6.600 Y ANKRD20A4 n/a
8 TRCN0000255625 TGATTGCCATGGAGACTTAAA pLKO_005 2562 3UTR 100% 13.200 6.600 Y ANKRD20A20P n/a
9 TRCN0000262909 ACTATGACTCACCAGGTATTG pLKO_005 529 3UTR 100% 10.800 5.400 Y ANKRD20A4 n/a
10 TRCN0000262905 TGTGAAAGGAGCAGTACAAAG pLKO_005 852 3UTR 100% 10.800 5.400 Y ANKRD20A4 n/a
11 TRCN0000155962 CTGTGAAAGGAGCAGTACAAA pLKO.1 851 3UTR 100% 5.625 2.813 Y ANKRD20A11P n/a
12 TRCN0000128666 CCAATCCAAACCTTAAGGATA pLKO.1 277 3UTR 100% 4.950 2.475 Y ANKRD20A2 n/a
13 TRCN0000133759 CCAATCCAAACCTTAAGGATA pLKO.1 277 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
14 TRCN0000128015 GCTGAGAATACAAGGCTCAAT pLKO.1 1564 3UTR 100% 4.950 2.475 Y ANKRD20A2 n/a
15 TRCN0000136726 GCTGAGAATACAAGGCTCAAT pLKO.1 1564 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
16 TRCN0000172688 GCTGGCAGATTAACCCAACAA pLKO.1 1051 3UTR 100% 4.950 2.475 Y ANKRD20A1 n/a
17 TRCN0000156881 GCTGTGAAAGGAGCAGTACAA pLKO.1 850 3UTR 100% 4.950 2.475 Y ANKRD20A11P n/a
18 TRCN0000153376 GCCAATCCAAACCTTAAGGAT pLKO.1 276 3UTR 100% 3.000 1.500 Y ANKRD20A5P n/a
19 TRCN0000157682 CCTTTGATACAGGCTGTCCAT pLKO.1 213 3UTR 100% 2.640 1.320 Y ANKRD20A5P n/a
20 TRCN0000156507 GTATAGTGAGAGCACCTCACT pLKO.1 329 3UTR 100% 0.264 0.132 Y ANKRD20A5P n/a
21 TRCN0000139212 CAGCACAGAACTGCTCTACAT pLKO.1 102 3UTR 100% 4.950 2.475 Y ANKRD20A19P n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_003366.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13700 pDONR223 100% 58.2% None (many diffs) n/a
2 ccsbBroad304_13700 pLX_304 0% 58.2% V5 (many diffs) n/a
3 TRCN0000476704 CAATTCCTCTTAAGATTAAATCAT pLX_317 15.4% 58.2% V5 (many diffs) n/a
4 ccsbBroadEn_13693 pDONR223 100% 10.1% None (many diffs) n/a
5 ccsbBroadEn_13719 pDONR223 100% 8% None (many diffs) n/a
6 ccsbBroad304_13719 pLX_304 0% 8% V5 (many diffs) n/a
7 TRCN0000467491 ATCAGATGCCTACGAAGGCAGTTC pLX_317 93.9% 8% V5 (many diffs) n/a
8 ccsbBroadEn_13631 pDONR223 100% 3.9% None (many diffs) n/a
9 ccsbBroad304_13631 pLX_304 0% 3.9% V5 (many diffs) n/a
10 TRCN0000477773 GCACCGACAGTTTAATGATTATCA pLX_317 100% 3.9% V5 (many diffs) n/a
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