Transcript: Human NR_040113.1

Homo sapiens ankyrin repeat domain 20 family member A5, pseudogene (ANKRD20A5P), non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
ANKRD20A5P (440482)
Length:
3707
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_040113.1
NBCI Gene record:
ANKRD20A5P (440482)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_040113.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000157683 CATGGTGCCAATCCAAACCTT pLKO.1 662 3UTR 100% 3.000 1.800 N ANKRD20A5P n/a
2 TRCN0000262908 ATTAGGCACAGAGTCATATTT pLKO_005 3221 3UTR 100% 15.000 7.500 Y ANKRD20A4 n/a
3 TRCN0000419116 ATATCCAAAGAGGCTTTATTG pLKO_005 2586 3UTR 100% 13.200 6.600 Y ANKRD20A1 n/a
4 TRCN0000434616 CATCAGAAAGAGGATCATAAA pLKO_005 2552 3UTR 100% 13.200 6.600 Y ANKRD20A1 n/a
5 TRCN0000157113 GAAGAGGCTTGTGCCGTTATT pLKO.1 632 3UTR 100% 13.200 6.600 Y ANKRD20A5P n/a
6 TRCN0000121674 GCACAGAACTGCTCTACATTT pLKO.1 496 3UTR 100% 13.200 6.600 Y ANKRD20A19P n/a
7 TRCN0000134343 GCATGATAGATGCACCTTAAA pLKO.1 1605 3UTR 100% 13.200 6.600 Y ANKRD20A3 n/a
8 TRCN0000423507 GGTATCAACAAGAGCTTAATG pLKO_005 2015 3UTR 100% 13.200 6.600 Y ANKRD20A1 n/a
9 TRCN0000262906 TCATCAGAAAGAGGATCATAA pLKO_005 2551 3UTR 100% 13.200 6.600 Y ANKRD20A4 n/a
10 TRCN0000155962 CTGTGAAAGGAGCAGTACAAA pLKO.1 1332 3UTR 100% 5.625 2.813 Y ANKRD20A11P n/a
11 TRCN0000139212 CAGCACAGAACTGCTCTACAT pLKO.1 494 3UTR 100% 4.950 2.475 Y ANKRD20A19P n/a
12 TRCN0000128666 CCAATCCAAACCTTAAGGATA pLKO.1 669 3UTR 100% 4.950 2.475 Y ANKRD20A2 n/a
13 TRCN0000133759 CCAATCCAAACCTTAAGGATA pLKO.1 669 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
14 TRCN0000156157 CCTAGCACTGAAATGGAAGAT pLKO.1 1307 3UTR 100% 4.950 2.475 Y ANKRD20A11P n/a
15 TRCN0000158171 CCTTGAAGCCTAGCACTGAAA pLKO.1 1299 3UTR 100% 4.950 2.475 Y ANKRD20A11P n/a
16 TRCN0000138952 GCCGTTATTCTGCTGGAACAT pLKO.1 644 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
17 TRCN0000128015 GCTGAGAATACAAGGCTCAAT pLKO.1 2044 3UTR 100% 4.950 2.475 Y ANKRD20A2 n/a
18 TRCN0000136726 GCTGAGAATACAAGGCTCAAT pLKO.1 2044 3UTR 100% 4.950 2.475 Y ANKRD20A3 n/a
19 TRCN0000156881 GCTGTGAAAGGAGCAGTACAA pLKO.1 1331 3UTR 100% 4.950 2.475 Y ANKRD20A11P n/a
20 TRCN0000153376 GCCAATCCAAACCTTAAGGAT pLKO.1 668 3UTR 100% 3.000 1.500 Y ANKRD20A5P n/a
21 TRCN0000157682 CCTTTGATACAGGCTGTCCAT pLKO.1 605 3UTR 100% 2.640 1.320 Y ANKRD20A5P n/a
22 TRCN0000140773 GAACTGCTCTACATTTGGCCT pLKO.1 501 3UTR 100% 0.660 0.330 Y ANKRD20A19P n/a
23 TRCN0000157562 GAACTGCTCTACATTTGGCCT pLKO.1 501 3UTR 100% 0.660 0.330 Y ANKRD20A5P n/a
24 TRCN0000156507 GTATAGTGAGAGCACCTCACT pLKO.1 721 3UTR 100% 0.264 0.132 Y ANKRD20A5P n/a
25 TRCN0000262905 TGTGAAAGGAGCAGTACAAAG pLKO_005 1333 3UTR 100% 10.800 5.400 Y ANKRD20A4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_040113.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13700 pDONR223 100% 62.6% None (many diffs) n/a
2 ccsbBroad304_13700 pLX_304 0% 62.6% V5 (many diffs) n/a
3 TRCN0000476704 CAATTCCTCTTAAGATTAAATCAT pLX_317 15.4% 62.6% V5 (many diffs) n/a
4 ccsbBroadEn_13693 pDONR223 100% 13.3% None (many diffs) n/a
5 ccsbBroadEn_13719 pDONR223 100% 10.4% None (many diffs) n/a
6 ccsbBroad304_13719 pLX_304 0% 10.4% V5 (many diffs) n/a
7 TRCN0000467491 ATCAGATGCCTACGAAGGCAGTTC pLX_317 93.9% 10.4% V5 (many diffs) n/a
8 ccsbBroadEn_13664 pDONR223 100% 7.3% None (many diffs) n/a
9 ccsbBroad304_13664 pLX_304 0% 7.3% V5 (many diffs) n/a
10 TRCN0000477985 GGTTGACGTCGGCTACCACGCATT pLX_317 100% 7.3% V5 (many diffs) n/a
11 ccsbBroadEn_13631 pDONR223 100% 4% None (many diffs) n/a
12 ccsbBroad304_13631 pLX_304 0% 4% V5 (many diffs) n/a
13 TRCN0000477773 GCACCGACAGTTTAATGATTATCA pLX_317 100% 4% V5 (many diffs) n/a
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