Construct: ORF TRCN0000467504
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001173.1_s317c1
- Derived from:
- ccsbBroadEn_10861
- DNA Barcode:
- GGTCGTTCCCTAAGCAGGAAGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRID1 (2894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467504
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2894 | GRID1 | glutamate ionotropic recept... | NM_017551.3 | 12.8% | 12.8% | 1_2637del |
| 2 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_006518567.3 | 16.2% | 17.2% | (many diffs) |
| 3 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_006518566.3 | 14.8% | 15.7% | (many diffs) |
| 4 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | XM_006518565.3 | 12.9% | 13.7% | (many diffs) |
| 5 | mouse | 14803 | Grid1 | glutamate receptor, ionotro... | NM_008166.2 | 11.7% | 12.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cagcctCATG GATGAAGACA TTGCTCACAA GCAGATTTCC CCAGCGTCGA 121 TTGAGCTCTC GGCCCTGGAG ATGGGGGGCC TGGCTCCCAC CCAGACCTTG GAGCCGACAC 181 GGGAGTACCA GAACACCCAG CTCTCGGTCA GCACCTTTCT GCCAGAGCAG AGCAGCCATG 241 GCACCAGCCG GACACTCTCA TCAGGGCCCA GCAGCAACCT GCCGCTGCCG CTGAGCAGCT 301 CGGCGACCAT GCCCTCCATG CAGTGCAAAC ACAGGTCACC CAACGGGGGG CTGTTCCGGC 361 AGAGCCCGGT GAAGACCCCC ATCCCCATGT CCTTCCAGCC CGTGCCTGGA GGCGTCCTTC 421 CAGAGGCTCT GGACACCTCC CACGGGACCT CCATCTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AGGTCGTTCC CTAAGCAGGA AGAATACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt