Construct: ORF TRCN0000467585
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014985.1_s317c1
- Derived from:
- ccsbBroadEn_02972
- DNA Barcode:
- CGTTGCGCGATTGGCGCAGTTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAGE2E (26749)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467585
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 729408 | GAGE2D | G antigen 2D | NM_001098407.1 | 100% | 100% | |
| 2 | human | 26749 | GAGE2E | G antigen 2E | NM_001127200.3 | 100% | 100% | |
| 3 | human | 100101629 | GAGE8 | G antigen 8 | NM_012196.1 | 100% | 100% | |
| 4 | human | 645037 | GAGE2B | G antigen 2B | NM_001098411.3 | 99.7% | 99.1% | 220C>G |
| 5 | human | 2574 | GAGE2C | G antigen 2C | NM_001472.2 | 99.7% | 99.1% | 220C>G |
| 6 | human | 729447 | GAGE2A | G antigen 2A | NM_001127212.1 | 99.4% | 98.2% | 175C>G;220C>G |
| 7 | human | 645051 | GAGE13 | G antigen 13 | NM_001098412.2 | 98.8% | 98.2% | 25_27delTAT;31T>C |
| 8 | human | 2576 | GAGE4 | G antigen 4 | NM_001474.1 | 98% | 97.4% | (many diffs) |
| 9 | human | 729396 | GAGE12J | G antigen 12J | NM_001098406.2 | 98% | 96.5% | (many diffs) |
| 10 | human | 100008586 | GAGE12F | G antigen 12F | NM_001098405.2 | 97.7% | 96.5% | (many diffs) |
| 11 | human | 645073 | GAGE12G | G antigen 12G | NM_001098409.2 | 97.7% | 96.5% | (many diffs) |
| 12 | human | 729428 | GAGE12B | G antigen 12B | NM_001127345.2 | 97.7% | 96.5% | (many diffs) |
| 13 | human | 2577 | GAGE5 | G antigen 5 | NM_001475.1 | 97.7% | 96.5% | (many diffs) |
| 14 | human | 26748 | GAGE12I | G antigen 12I | NM_001477.1 | 97.7% | 96.5% | (many diffs) |
| 15 | human | 2579 | GAGE7 | G antigen 7 | NM_021123.3 | 97.7% | 96.5% | (many diffs) |
| 16 | human | 2543 | GAGE1 | G antigen 1 | NM_001040663.2 | 97.4% | 96.5% | (many diffs) |
| 17 | human | 729422 | GAGE12C | G antigen 12C | NM_001098408.1 | 97.4% | 95.7% | (many diffs) |
| 18 | human | 729431 | GAGE12E | G antigen 12E | NM_001098418.1 | 97.4% | 95.7% | (many diffs) |
| 19 | human | 100132399 | GAGE12D | G antigen 12D | NM_001127199.1 | 97.4% | 95.7% | (many diffs) |
| 20 | human | 2578 | GAGE6 | G antigen 6 | NM_001476.1 | 97.4% | 95.7% | (many diffs) |
| 21 | human | 102724473 | GAGE10 | G antigen 10 | NM_001098413.3 | 97.4% | 93.9% | (many diffs) |
| 22 | human | 729442 | GAGE12H | G antigen 12H | NM_001098410.1 | 97.1% | 94.8% | (many diffs) |
| 23 | human | 2543 | GAGE1 | G antigen 1 | XM_011543896.2 | 88.4% | 87.9% | (many diffs) |
| 24 | human | 102724473 | GAGE10 | G antigen 10 | XM_024452325.1 | 77.9% | 73.2% | (many diffs) |
| 25 | human | 645073 | GAGE12G | G antigen 12G | NM_001354422.1 | 62.1% | 61.5% | (many diffs) |
| 26 | human | 2543 | GAGE1 | G antigen 1 | NR_102272.1 | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttggcgagga agatcgacct atcggccTAG ACCAAGACGC TACGTAGAGC 121 CTCCTGAAAT GATTGGGCCT ATGCGGCCCG AGCAGTTCAG TGATGAAGTG GAACCAGCAA 181 CACCTGAAGA AGGGGAACCA GCAACTCAAC GTCAGGATCC TGCAGCTGCT CAGGAGGGAG 241 AGGATGAGGG AGCATCTGCA GGTCAAGGGC CGAAGCCTGA AGCTGATAGC CAGGAACAGG 301 GTCACCCACA GACTGGGTGT GAGTGTGAAG ATGGTCCTGA TGGGCAGGAG ATGGACCCGC 361 CAAATCCAGA GGAGGTGAAA ACGCCTGAAG AAGGTGAAAA GCAATCACAG TGTTACCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAC GTTGCGCGAT TGGCGCAGTT ACTACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att