Construct: ORF TRCN0000467586
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012918.1_s317c1
- Derived from:
- ccsbBroadEn_07114
- DNA Barcode:
- GAATGGAAAAAATCAAATTTAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2D3 (7323)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467586
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_003340.6 | 99.7% | 99.3% | 7C>G |
| 2 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181886.3 | 99.7% | 99.3% | 7C>G |
| 3 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181887.2 | 99.7% | 99.3% | 7C>G |
| 4 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181888.3 | 99.7% | 99.3% | 7C>G |
| 5 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181889.2 | 99.7% | 99.3% | 7C>G |
| 6 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181890.2 | 99.7% | 99.3% | 7C>G |
| 7 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181891.3 | 99.7% | 99.3% | 7C>G |
| 8 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_024454202.1 | 99.7% | 99.3% | 7C>G |
| 9 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181893.2 | 96.2% | 95.3% | (many diffs) |
| 10 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_181892.3 | 96.1% | 96.6% | (many diffs) |
| 11 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | NM_003339.3 | 86.3% | 96.5% | (many diffs) |
| 12 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | NM_001300795.1 | 80.2% | 80.2% | 0_1ins87 |
| 13 | human | 7323 | UBE2D3 | ubiquitin conjugating enzym... | XM_017008584.2 | 80.2% | 80.2% | 0_1ins87 |
| 14 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | NM_015983.4 | 76.8% | 91.1% | (many diffs) |
| 15 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | XM_017009820.1 | 73.7% | 76.9% | (many diffs) |
| 16 | human | 7322 | UBE2D2 | ubiquitin conjugating enzym... | NM_181838.1 | 70.7% | 78.9% | (many diffs) |
| 17 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_024446796.1 | 58.7% | 69.3% | (many diffs) |
| 18 | human | 51619 | UBE2D4 | ubiquitin conjugating enzym... | XM_011515422.3 | 58.2% | 67.3% | (many diffs) |
| 19 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | NM_025356.4 | 98.1% | 99.3% | (many diffs) |
| 20 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | XM_006501873.2 | 98.1% | 99.3% | (many diffs) |
| 21 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | XM_006501874.2 | 98.1% | 99.3% | (many diffs) |
| 22 | mouse | 66105 | Ube2d3 | ubiquitin-conjugating enzym... | XM_006501875.2 | 98.1% | 99.3% | (many diffs) |
| 23 | mouse | 56550 | Ube2d2a | ubiquitin-conjugating enzym... | NM_019912.2 | 85.4% | 96.5% | (many diffs) |
| 24 | mouse | 73318 | Ube2d2b | ubiquitin-conjugating enzym... | NM_001276397.1 | 82.9% | 90.4% | (many diffs) |
| 25 | mouse | 56550 | Ube2d2a | ubiquitin-conjugating enzym... | XM_006526120.2 | 70.5% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtgaaacgg attaataagg aacttagtga tttggcccgt gaccctccag 121 cacaatgttc tgcaggtcca gttggggatg atatgtttca ttGGCAAGCC ACAATTATGG 181 GACCTAATGA CAGCCCATAT CAAGGCGGTG TATTCTTTTT GACAATTCAT TTTCCTACAG 241 ACTACCCCTT CAAACCACCT AAGGTTGCAT TTACAACAAG AATTTATCAT CCAAATATTA 301 ACAGTAATGG CAGCATTTGT CTCGATATTC TAAGATCACA GTGGTCGCCT GCTTTAACAA 361 TTTCTAAAGT TCTTTTATCC ATTTGTTCAC TGCTATGTGA TCCAAACCCA GATGACCCCC 421 TAGTGCCAGA GATTGCACGG ATCTATAAAA CAGACAGAGA TAAGTACAAC AGAATATCTC 481 GGGAATGGAC TCAGAAGTAT GCCATGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAATGGAA 661 AAAATCAAAT TTAACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt