Construct: ORF TRCN0000467672
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013729.1_s317c1
- Derived from:
- ccsbBroadEn_01027
- DNA Barcode:
- CTTTGCTCACATTTCAGAGTCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOBP (4336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467672
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4336 | MOBP | myelin associated oligodend... | NM_182935.4 | 100% | 100% | |
| 2 | human | 4336 | MOBP | myelin associated oligodend... | NM_001278323.1 | 40.4% | 38.7% | (many diffs) |
| 3 | human | 4336 | MOBP | myelin associated oligodend... | NM_001278322.2 | 37.9% | 34.9% | (many diffs) |
| 4 | human | 4336 | MOBP | myelin associated oligodend... | NR_003090.3 | 4.2% | 1_149del;393_5717del | |
| 5 | human | 4336 | MOBP | myelin associated oligodend... | NR_103504.2 | 3.7% | (many diffs) | |
| 6 | human | 4336 | MOBP | myelin associated oligodend... | NR_103505.2 | 3.7% | (many diffs) | |
| 7 | mouse | 17433 | Mobp | myelin-associated oligodend... | NM_001039364.2 | 87.6% | 90.1% | (many diffs) |
| 8 | mouse | 17433 | Mobp | myelin-associated oligodend... | NM_008614.2 | 87.6% | 90.1% | (many diffs) |
| 9 | mouse | 17433 | Mobp | myelin-associated oligodend... | NM_001039365.2 | 40.8% | 39.4% | (many diffs) |
| 10 | mouse | 17433 | Mobp | myelin-associated oligodend... | XR_379851.1 | 8.4% | (many diffs) | |
| 11 | mouse | 17433 | Mobp | myelin-associated oligodend... | XR_871174.1 | 7.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 213
- ORF end:
- 456
- ORF length:
- 243
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGAGTCA GAAACCGGCC AAGGAGGGTC 241 CCAGACTCTC CAAAAACCAG AAGTACTCCG AACACTTCAG CATACACTGC TGCCCGCCGT 301 TCACCTTCCT CAATTCCAAG AAGGAGATAG TGGATCGGAA ATACAGCATC TGTAAGAGCG 361 GCTGCTTCTA CCAGAAGAAA GAGGAGGACT GGATCTGCTG CGCCTGCCAG AAGACCAGAT 421 TGAAAAGGAA GATCAGGCCA ACCCCAAAGA AGAAGTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 ACTTTGCTCA CATTTCAGAG TCAAAACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt