Construct: ORF TRCN0000467892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004448.1_s317c1
- Derived from:
- ccsbBroadEn_00095
- DNA Barcode:
- AAGACCTTCCAAGGGGGGATCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARF3 (377)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 377 | ARF3 | ADP ribosylation factor 3 | NM_001659.3 | 99.8% | 100% | 117C>G |
| 2 | human | 377 | ARF3 | ADP ribosylation factor 3 | XM_005268856.1 | 99.8% | 100% | 117C>G |
| 3 | human | 377 | ARF3 | ADP ribosylation factor 3 | XM_024448972.1 | 99.8% | 100% | 117C>G |
| 4 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001024226.1 | 83.9% | 96.1% | (many diffs) |
| 5 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001024227.1 | 83.9% | 96.1% | (many diffs) |
| 6 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001024228.2 | 83.9% | 96.1% | (many diffs) |
| 7 | human | 375 | ARF1 | ADP ribosylation factor 1 | NM_001658.4 | 83.9% | 96.1% | (many diffs) |
| 8 | human | 377 | ARF3 | ADP ribosylation factor 3 | XM_006719391.4 | 74.2% | 70.7% | (many diffs) |
| 9 | mouse | 11842 | Arf3 | ADP-ribosylation factor 3 | NM_007478.3 | 92.8% | 100% | (many diffs) |
| 10 | mouse | 11842 | Arf3 | ADP-ribosylation factor 3 | XM_011245445.1 | 92.8% | 100% | (many diffs) |
| 11 | mouse | 11840 | Arf1 | ADP-ribosylation factor 1 | NM_001130408.1 | 82.8% | 96.1% | (many diffs) |
| 12 | mouse | 11840 | Arf1 | ADP-ribosylation factor 1 | NM_007476.3 | 82.8% | 96.1% | (many diffs) |
| 13 | mouse | 11841 | Arf2 | ADP-ribosylation factor 2 | NM_001304574.1 | 81.3% | 94.4% | (many diffs) |
| 14 | mouse | 11841 | Arf2 | ADP-ribosylation factor 2 | NM_007477.5 | 81.3% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caatatcttt ggaaaccttc tcaagagcct gattgggaag aaggagatgc 121 gcatcctgat ggtgggccTG GATGCCGCAG GAAAGACCAC CATCCTATAC AAGCTGAAAC 181 TGGGGGAGAT CGTCACCACC ATCCCTACCA TTGGGTTCAA TGTGGAGACA GTGGAGTATA 241 AGAACATCAG CTTTACAGTG TGGGATGTGG GTGGCCAGGA CAAGATTCGA CCCCTCTGGA 301 GACACTACTT CCAGAACACC CAAGGGTTGA TATTTGTGGT CGACAGCAAT GATCGGGAGC 361 GAGTAAATGA GGCCCGGGAA GAGCTGATGA GAATGCTGGC GGAGGACGAG CTCCGGGATG 421 CTGTACTCCT TGTCTTTGCA AACAAACAGG ATCTGCCTAA TGCTATGAAC GCTGCTGAGA 481 TCACAGACAA GCTGGGCCTG CATTCCCTTC GTCACCGTAA CTGGTACATT CAGGCCACCT 541 GTGCCACCAG CGGGGACGGG CTGTACGAAG GCCTGGACTG GCTGGCCAAT CAGCTCAAAA 601 ACAAGAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGACC TTCCAAGGGG GGATCTAGAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt