Transcript: Human NM_001024226.1

Homo sapiens ADP ribosylation factor 1 (ARF1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
ARF1 (375)
Length:
1986
CDS:
229..774

Additional Resources:

NCBI RefSeq record:
NM_001024226.1
NBCI Gene record:
ARF1 (375)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001024226.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039876 CAATGACAGAGAGCGTGTGAA pLKO.1 510 CDS 100% 4.950 3.465 N ARF1 n/a
2 TRCN0000288643 CAATGACAGAGAGCGTGTGAA pLKO_005 510 CDS 100% 4.950 3.465 N ARF1 n/a
3 TRCN0000009822 CAATTCTGCATGGTCACAGTA pLKO.1 1337 3UTR 100% 4.950 3.465 N ARF1 n/a
4 TRCN0000039873 CCTAGCATAGATTTGCAGTTA pLKO.1 1241 3UTR 100% 4.950 3.465 N ARF1 n/a
5 TRCN0000288585 CCTAGCATAGATTTGCAGTTA pLKO_005 1241 3UTR 100% 4.950 3.465 N ARF1 n/a
6 TRCN0000039874 GAAGACCACGATCCTCTACAA pLKO.1 315 CDS 100% 4.950 3.465 N ARF1 n/a
7 TRCN0000288583 GAAGACCACGATCCTCTACAA pLKO_005 315 CDS 100% 4.950 3.465 N ARF1 n/a
8 TRCN0000010298 CAAGAACATCAGCTTCACTGT pLKO.1 402 CDS 100% 2.640 1.848 N ARF1 n/a
9 TRCN0000039877 GCACTCACTACGCCACAGGAA pLKO.1 663 CDS 100% 0.880 0.616 N ARF1 n/a
10 TRCN0000288645 GCACTCACTACGCCACAGGAA pLKO_005 663 CDS 100% 0.880 0.616 N ARF1 n/a
11 TRCN0000039875 CCTCCTGGTGTTCGCCAACAA pLKO.1 588 CDS 100% 1.650 0.990 N ARF1 n/a
12 TRCN0000288584 CCTCCTGGTGTTCGCCAACAA pLKO_005 588 CDS 100% 1.650 0.990 N ARF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001024226.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00094 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00094 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470051 AGACATCCAACAGCAAGTTGGGTG pLX_317 77.4% 100% 100% V5 n/a
4 ccsbBroadEn_00095 pDONR223 100% 83.9% 96.1% None (many diffs) n/a
5 ccsbBroad304_00095 pLX_304 0% 83.9% 96.1% V5 (many diffs) n/a
6 TRCN0000467892 AAGACCTTCCAAGGGGGGATCTAG pLX_317 85.4% 83.9% 96.1% V5 (many diffs) n/a
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