Construct: ORF TRCN0000467901
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003414.1_s317c1
- Derived from:
- ccsbBroadEn_02387
- DNA Barcode:
- AATAATGACTCCTACGGATTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPEG (10290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467901
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10290 | SPEG | striated muscle enriched pr... | NM_001173476.2 | 100% | 100% | |
| 2 | human | 10290 | SPEG | striated muscle enriched pr... | XM_024452526.1 | 100% | 100% | |
| 3 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003162.2 | 66.4% | 66.4% | 1_171del |
| 4 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003161.2 | 62.5% | 62.2% | (many diffs) |
| 5 | human | 10290 | SPEG | striated muscle enriched pr... | XR_001738587.2 | 7% | (many diffs) | |
| 6 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003159.2 | 6.1% | 6% | (many diffs) |
| 7 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246240.2 | 4.6% | 4.5% | 335_342delCACACCCT;344_345delAAinsCG;348_7254del |
| 8 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246241.1 | 4.6% | 4.5% | 335_342delCACACCCT;344_345delAAinsCG;348_7254del |
| 9 | human | 10290 | SPEG | striated muscle enriched pr... | XM_006712193.3 | 4.6% | 4.5% | 335_342delCACACCCT;344_345delAAinsCG;348_7254del |
| 10 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003158.2 | 4.6% | 4.5% | 335_342delCACACCCT;344_345delAAinsCG;348_7254del |
| 11 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246239.2 | 4.5% | 4.4% | (many diffs) |
| 12 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246242.4 | 4.5% | 4.4% | (many diffs) |
| 13 | human | 10290 | SPEG | striated muscle enriched pr... | XM_006712189.3 | 3.5% | 3.5% | (many diffs) |
| 14 | human | 10290 | SPEG | striated muscle enriched pr... | XM_005246237.2 | 3.5% | 3.4% | (many diffs) |
| 15 | human | 10290 | SPEG | striated muscle enriched pr... | XM_017003157.1 | 3.5% | 3.4% | (many diffs) |
| 16 | human | 10290 | SPEG | striated muscle enriched pr... | XM_011510483.2 | 3.5% | 3.4% | (many diffs) |
| 17 | human | 10290 | SPEG | striated muscle enriched pr... | NM_005876.5 | 3.4% | 3.3% | (many diffs) |
| 18 | human | 10290 | SPEG | striated muscle enriched pr... | XM_011510479.2 | 3.4% | 3.3% | (many diffs) |
| 19 | mouse | 11790 | Speg | SPEG complex locus | NM_001173477.1 | 87.6% | 94.6% | (many diffs) |
| 20 | mouse | 11790 | Speg | SPEG complex locus | XM_017312276.1 | 11.6% | 12.5% | (many diffs) |
| 21 | mouse | 11790 | Speg | SPEG complex locus | NM_001085371.1 | 11.4% | 12.4% | (many diffs) |
| 22 | mouse | 11790 | Speg | SPEG complex locus | XM_011238651.2 | 10.1% | 10.9% | (many diffs) |
| 23 | mouse | 11790 | Speg | SPEG complex locus | XM_011238650.2 | 5.6% | 6% | (many diffs) |
| 24 | mouse | 11790 | Speg | SPEG complex locus | XM_006496403.3 | 4% | 4.3% | (many diffs) |
| 25 | mouse | 11790 | Speg | SPEG complex locus | XM_006496404.3 | 4% | 4.3% | (many diffs) |
| 26 | mouse | 11790 | Speg | SPEG complex locus | XM_006496402.2 | 3.9% | 4.2% | (many diffs) |
| 27 | mouse | 11790 | Speg | SPEG complex locus | NM_001085370.1 | 3.8% | 4.1% | (many diffs) |
| 28 | mouse | 11790 | Speg | SPEG complex locus | XM_006496400.2 | 3.8% | 4.1% | (many diffs) |
| 29 | mouse | 11790 | Speg | SPEG complex locus | XM_006496401.2 | 3.8% | 4.1% | (many diffs) |
| 30 | mouse | 11790 | Speg | SPEG complex locus | XM_011238648.2 | 3.8% | 4.1% | (many diffs) |
| 31 | mouse | 11790 | Speg | SPEG complex locus | XM_011238649.2 | 3.8% | 4.1% | (many diffs) |
| 32 | mouse | 11790 | Speg | SPEG complex locus | XM_011238647.1 | 3.1% | 3.3% | (many diffs) |
| 33 | mouse | 11790 | Speg | SPEG complex locus | XM_017322375.1 | 3.1% | 3.3% | (many diffs) |
| 34 | mouse | 11790 | Speg | SPEG complex locus | XM_011238646.1 | 3.1% | 3.3% | (many diffs) |
| 35 | mouse | 11790 | Speg | SPEG complex locus | XM_011238645.1 | 3.1% | 3.3% | (many diffs) |
| 36 | mouse | 11790 | Speg | SPEG complex locus | XM_017322354.1 | 3% | 3.2% | (many diffs) |
| 37 | mouse | 11790 | Speg | SPEG complex locus | XM_011238644.1 | 3% | 3.2% | (many diffs) |
| 38 | mouse | 11790 | Speg | SPEG complex locus | NM_007463.4 | 3% | 3.2% | (many diffs) |
| 39 | mouse | 11790 | Speg | SPEG complex locus | XM_011238643.1 | 3% | 3.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcccagtccc agccagaacc gccgttcttc tgacactggc tccaaggcac 121 cccccacctt caaggTCTCA CTTATGGACC AGTCAGTAAG AGAAGGCCAA GATGTCATCA 181 TGAGCATCCG CGTGCAGGGG GAGCCCAAGC CTGTGGTCTC CTGGCTGAGA AACCGCCAGC 241 CCGTGCGCCC AGACCAGCGG CGCTTTGCGG AGGAGGCTGA GGGTGGGCTG TGCCGGCTGC 301 GGATCCTGGC TGCAGAGCGT GGCGATGCTG GTTTCTACAC TTGCAAAGCG GTCAATGAGT 361 ATGGTGCTCG GCAGTGCGAG GCCCGCTTGG AGGTCCGAGG CGAGTGCCCA ACTTTCTTGT 421 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 481 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 541 GAAAGGACGA AATAATGACT CCTACGGATT TTGCACGCGT TAAGTCgaca atcaacctct 601 ggattacaaa atttgtgaaa gatt