Construct: ORF TRCN0000468230
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002399.1_s317c1
- Derived from:
- ccsbBroadEn_00412
- DNA Barcode:
- ATCAACTGGCCTGCGCCACCCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP3A5 (1577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468230
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_000777.5 | 100% | 100% | |
| 2 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001291830.2 | 96.9% | 96% | (many diffs) |
| 3 | human | 1576 | CYP3A4 | cytochrome P450 family 3 su... | NM_017460.6 | 89.2% | 84% | (many diffs) |
| 4 | human | 1576 | CYP3A4 | cytochrome P450 family 3 su... | NM_001202855.3 | 89.2% | 84.2% | (many diffs) |
| 5 | human | 1551 | CYP3A7 | cytochrome P450 family 3 su... | NM_000765.5 | 88.5% | 81.7% | (many diffs) |
| 6 | human | 100861540 | CYP3A7-CYP3A51P | CYP3A7-CYP3A51P readthrough | NM_001256497.3 | 82.9% | 76.4% | (many diffs) |
| 7 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001291829.2 | 77.4% | 77.4% | 0_1ins339 |
| 8 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NR_033807.3 | 45.3% | (many diffs) | |
| 9 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001190484.3 | 25.1% | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1572
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctcatccca aatttggcgg tggaaacctg gcttctcctg gctgtcagcc 121 tggtgctcct ctatctatat gggacccgta cacatggact ttttaagaga ctgggaattc 181 cagggcccac acctctgcct ttgttgggaa atgttttgtc ctatcgtcag ggtctctgga 241 aatttgacac agagtgctat aaaaagtatg gaaaaatgtg gggaacgtat gaaggtcaac 301 tccctgtgct ggccatcaca gatcccgacg tgatcagaac agtgctagtg aaagaatgtt 361 attctgtctt cacaaatcga aggtctttag gcccagtggg atttatgaaa agtgccatct 421 ctttagctga ggatgaagaa tggaagagaa tacggtcatt gctgtctcca accttcacca 481 gcggaaaact caaggagatg ttccccatca ttgcccagta tggagatgta ttggtgagaa 541 acttgaggcg ggaagcagag aaaggcaagc ctgtcacctt gaaagacatc tttggggcct 601 acagcatgga tgtgattact ggcacatcat ttggagtgaa catcgactct ctcaacaatc 661 cacaagaccc ctttgtggag agcactaaga agttcctaaa atttggtttc ttagatccat 721 tatttctctc aataatactc tttccattcc ttaccccagt ttttgaagca ttaaatgtct 781 ctctgtttcc aaaagatacc ataaattttt taagtaaatc tgtaaacaga atgaagaaaa 841 gtcgcctcaa cgacaaacaa aagcaccgac tagatttcct tcagctgatg attgactccc 901 agaattcgaa agaaactgag tcccacaaag ctctgtctga tctggagctc gcagcccagt 961 caataatctt catttttgct ggctatgaaa ccaccagcag tgttctttcc ttcactttat 1021 atgaactggc cactcaccct gatgtccagc agaaactgca aaaggagatt gatgcagttt 1081 tgcccaataa ggcaccacct accTATGATG CCGTGGTACA GATGGAGTAC CTTGACATGG 1141 TGGTGAATGA AACACTCAGA TTATTCCCAG TTGCTATTAG ACTTGAGAGG ACTTGCAAGA 1201 AAGATGTTGA AATCAATGGG GTATTCATTC CCAAAGGGTC AATGGTGGTG ATTCCAACTT 1261 ATGCTCTTCA CCATGACCCA AAGTACTGGA CAGAGCCTGA GGAGTTCCGC CCTGAAAGGT 1321 TCAGTAAGAA GAAGGACAGC ATAGATCCTT ACATATACAC ACCCTTTGGA ACTGGACCCA 1381 GAAACTGCAT TGGCATGAGG TTTGCTCTCA TGAACATGAA ACTTGCTCTA ATCAGAGTCC 1441 TTCAGAACTT CTCCTTCAAA CCTTGTAAAG AAACACAGAT CCCCTTGAAA TTAGACACGC 1501 AAGGACTTCT TCAACCAGAA AAACCCATTG TTCTAAAGGT GGATTCAAGA GATGGAACCC 1561 TAAGTGGAGA ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1621 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1681 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATC AACTGGCCTG CGCCACCCCT 1741 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t