Construct: ORF TRCN0000468230

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002399.1_s317c1
Derived from:
ccsbBroadEn_00412
DNA Barcode:
ATCAACTGGCCTGCGCCACCCCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CYP3A5 (1577)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468230
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1577 CYP3A5 cytochrome P450 family 3 su... NM_000777.5 100% 100%
2 human 1577 CYP3A5 cytochrome P450 family 3 su... NM_001291830.2 96.9% 96% (many diffs)
3 human 1576 CYP3A4 cytochrome P450 family 3 su... NM_017460.6 89.2% 84% (many diffs)
4 human 1576 CYP3A4 cytochrome P450 family 3 su... NM_001202855.3 89.2% 84.2% (many diffs)
5 human 1551 CYP3A7 cytochrome P450 family 3 su... NM_000765.5 88.5% 81.7% (many diffs)
6 human 100861540 CYP3A7-CYP3A51P CYP3A7-CYP3A51P readthrough NM_001256497.3 82.9% 76.4% (many diffs)
7 human 1577 CYP3A5 cytochrome P450 family 3 su... NM_001291829.2 77.4% 77.4% 0_1ins339
8 human 1577 CYP3A5 cytochrome P450 family 3 su... NR_033807.3 45.3% (many diffs)
9 human 1577 CYP3A5 cytochrome P450 family 3 su... NM_001190484.3 25.1% 22.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1572
ORF length:
1506
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cctcatccca aatttggcgg tggaaacctg gcttctcctg gctgtcagcc
121tggtgctcct ctatctatat gggacccgta cacatggact ttttaagaga ctgggaattc
181cagggcccac acctctgcct ttgttgggaa atgttttgtc ctatcgtcag ggtctctgga
241aatttgacac agagtgctat aaaaagtatg gaaaaatgtg gggaacgtat gaaggtcaac
301tccctgtgct ggccatcaca gatcccgacg tgatcagaac agtgctagtg aaagaatgtt
361attctgtctt cacaaatcga aggtctttag gcccagtggg atttatgaaa agtgccatct
421ctttagctga ggatgaagaa tggaagagaa tacggtcatt gctgtctcca accttcacca
481gcggaaaact caaggagatg ttccccatca ttgcccagta tggagatgta ttggtgagaa
541acttgaggcg ggaagcagag aaaggcaagc ctgtcacctt gaaagacatc tttggggcct
601acagcatgga tgtgattact ggcacatcat ttggagtgaa catcgactct ctcaacaatc
661cacaagaccc ctttgtggag agcactaaga agttcctaaa atttggtttc ttagatccat
721tatttctctc aataatactc tttccattcc ttaccccagt ttttgaagca ttaaatgtct
781ctctgtttcc aaaagatacc ataaattttt taagtaaatc tgtaaacaga atgaagaaaa
841gtcgcctcaa cgacaaacaa aagcaccgac tagatttcct tcagctgatg attgactccc
901agaattcgaa agaaactgag tcccacaaag ctctgtctga tctggagctc gcagcccagt
961caataatctt catttttgct ggctatgaaa ccaccagcag tgttctttcc ttcactttat
1021atgaactggc cactcaccct gatgtccagc agaaactgca aaaggagatt gatgcagttt
1081tgcccaataa ggcaccacct accTATGATG CCGTGGTACA GATGGAGTAC CTTGACATGG
1141TGGTGAATGA AACACTCAGA TTATTCCCAG TTGCTATTAG ACTTGAGAGG ACTTGCAAGA
1201AAGATGTTGA AATCAATGGG GTATTCATTC CCAAAGGGTC AATGGTGGTG ATTCCAACTT
1261ATGCTCTTCA CCATGACCCA AAGTACTGGA CAGAGCCTGA GGAGTTCCGC CCTGAAAGGT
1321TCAGTAAGAA GAAGGACAGC ATAGATCCTT ACATATACAC ACCCTTTGGA ACTGGACCCA
1381GAAACTGCAT TGGCATGAGG TTTGCTCTCA TGAACATGAA ACTTGCTCTA ATCAGAGTCC
1441TTCAGAACTT CTCCTTCAAA CCTTGTAAAG AAACACAGAT CCCCTTGAAA TTAGACACGC
1501AAGGACTTCT TCAACCAGAA AAACCCATTG TTCTAAAGGT GGATTCAAGA GATGGAACCC
1561TAAGTGGAGA ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1621ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1681CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATC AACTGGCCTG CGCCACCCCT
1741TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)