Construct: ORF TRCN0000468245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011814.2_s317c1
- Derived from:
- ccsbBroadEn_14960
- DNA Barcode:
- AGCAGTCATGCCACTATTTGGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK4 (10298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014831.3 | 100% | 100% | |
| 2 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014832.2 | 100% | 100% | |
| 3 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_005884.4 | 100% | 100% | |
| 4 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526316.1 | 100% | 100% | |
| 5 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526317.2 | 100% | 100% | |
| 6 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526318.2 | 100% | 100% | |
| 7 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526319.2 | 100% | 100% | |
| 8 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014834.3 | 74.1% | 74.1% | 202_203ins459 |
| 9 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014835.2 | 74.1% | 74.1% | 202_203ins459 |
| 10 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526320.1 | 74.1% | 74.1% | 202_203ins459 |
| 11 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_024451314.1 | 74.1% | 74.1% | 202_203ins459 |
| 12 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | NM_027470.3 | 86.2% | 92.5% | (many diffs) |
| 13 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312296.1 | 86.2% | 92.5% | (many diffs) |
| 14 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312297.1 | 86.2% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1839
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgggaagagg aagaagcggg tggagatctc cgcgccgtcc aacttcgagc 121 accgcgtgca cacgggcttc gaccagcacg agcagaagtt cacggggctg ccccgccagt 181 ggcagagcct gatcgaggag tcggctcgcc ggcccaagcc cctcgtcgac cccgcctgca 241 tcacctccat ccagcccggg gcccccaaga ccatcgtgcg gggcagcaaa ggtgccaaag 301 atggggccct cacgctgctg ctggacgagt ttgagaacat gtcggtgaca cgctccaact 361 ccctgcggag agacagcccg ccgccgcccg cccgtgcccg ccaggaaaat gggatgccag 421 aggagccggc caccacggcc agagggggcc cagggaaggc aggcagccga ggccggttcg 481 ccggtcacag cgaggcgggt ggcggcagtg gtgacaggcg acgggcgggg ccagagaaga 541 ggcccaagtc ttccagggag ggctcagggg gtccccagga gtcctcccgg gacaaacgcc 601 ccctctccgg gcctgatgtc ggcacccccc agcctgctgg tctggccagt ggggcgaaac 661 tggcagctgg ccggcccttt aacacctacc cgagggctga cacggaccac ccatcccggg 721 gtgcccaggg ggagcctcat gacgtggccc ctaacgggcc atcagcgggg ggcctggcca 781 tcccccagtc ctcctcctcc tcctcccggc ctcccacccg agcccgaggt gcccccagcc 841 ctggagtgct gggaccccac gcctcagagc cccagctggc ccctccagcc tgcacccccg 901 ccgcccctgc tgttcctggg ccccctggcc cccgctcacc acagcgggag ccacagcgag 961 tatcccatga gcagttccgg gctgccctgc agctggtggt ggacccaggc gacccccgct 1021 cctacctgga caacttcatc aagattggcg agggctccac gggcatcgtg tgcatcgcca 1081 ccgtgcgcag ctcgggcaag ctggtggccg tcaagaagat ggacctgcgc aagcagcaga 1141 ggcgcgagct gctcttcaac gaggtggtaa tcatgaggga ctaccagcac gagaatgtgg 1201 tggagatgta caacagctac ctggtggggg acgagctctg ggtggtcatg gagttcctgg 1261 aaggaggcgc cctcaccgac atcgtcaccc acaccaggat gaacgaggag cagatcgcgg 1321 ccgtgtgcct tgcagtgctg caggccctgt cggtgctcca cgcccagggc gtcatccacc 1381 gggacatcaa gagcgactcg atcctgctga cccatgatgg cagggtgaag ctgtcagact 1441 ttgggttctg cgcccaggtg agcaaggaag tgccccgaag gaagtcgctg gtcggcacgc 1501 cctactggat ggccccagag ctcaTCTCCC GCCTTCCCTA CGGGCCAGAG GTAGACATCT 1561 GGTCGCTGGG GATAATGGTG ATTGAGATGG TGGACGGAGA GCCCCCCTAC TTCAACGAGC 1621 CACCCCTCAA AGCCATGAAG ATGATTCGGG ACAACCTGCC ACCCCGACTG AAGAACCTGC 1681 ACAAGGTGTC GCCATCCCTG AAGGGCTTCC TGGACCGCCT GCTGGTGCGA GACCCTGCCC 1741 AGCGGGCCAC GGCAGCCGAG CTGCTGAAGC ACCCATTCCT GGCCAAGGCA GGGCCGCCTG 1801 CCAGCATCGT GCCCCTCATG CGCCAGAACC GCACCAGATG CCCAACTTTC TTGTACAAAG 1861 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1921 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1981 ACGAAGCAGT CATGCCACTA TTTGGTAAAC GCGTTAAGTC gacaatcaac ctctggatta 2041 caaaatttgt gaaagatt