Construct: ORF TRCN0000468306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005510.2_s317c1
- Derived from:
- ccsbBroadEn_00624
- DNA Barcode:
- GCGATAACTTATGCTACTCCATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCK (2645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2645 | GCK | glucokinase | NM_000162.5 | 100% | 100% | |
| 2 | human | 2645 | GCK | glucokinase | NM_001354800.1 | 98% | 98% | 1368_1369ins27 |
| 3 | human | 2645 | GCK | glucokinase | NM_033508.3 | 97.8% | 97.2% | (many diffs) |
| 4 | human | 2645 | GCK | glucokinase | NM_033507.3 | 97.7% | 97.4% | (many diffs) |
| 5 | human | 2645 | GCK | glucokinase | NM_001354803.1 | 29.1% | 27.3% | (many diffs) |
| 6 | human | 2645 | GCK | glucokinase | NM_001354801.1 | 27.3% | 27% | 1_1delAins1003;6_6delCinsGCAGGTGGAG |
| 7 | human | 2645 | GCK | glucokinase | XM_024446707.1 | 18.2% | 18.2% | 0_1ins1140 |
| 8 | human | 2645 | GCK | glucokinase | NM_001354802.1 | 16.3% | 16.3% | 0_1ins1140;228_229ins27 |
| 9 | mouse | 103988 | Gck | glucokinase | NM_010292.5 | 89% | 95.9% | (many diffs) |
| 10 | mouse | 103988 | Gck | glucokinase | NM_001287386.1 | 87.3% | 93.7% | (many diffs) |
| 11 | mouse | 103988 | Gck | glucokinase | XM_006514444.1 | 76.2% | 84.1% | (many diffs) |
| 12 | mouse | 103988 | Gck | glucokinase | XM_006514443.3 | 74.8% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggacgacaga gccaggatgg aggccgccaa gaaggagaag gtagagcaga 121 tcctggcaga gttccagctg caggaggagg acctgaagaa ggtgatgaga cggatgcaga 181 aggagatgga ccgcggcctg aggctggaga cccatgaaga ggccagtgtg aagatgctgc 241 ccacctacgt gcgctccacc ccagaaggct cagaagtcgg ggacttcctc tccctggacc 301 tgggtggcac taacttcagg gtgatgctgg tgaaggtggg agaaggtgag gaggggcagt 361 ggagcgtgaa gaccaaacac cagatgtact ccatccccga ggacgccatg accggcactg 421 ctgagatgct cttcgactac atctctgagt gcatctccga cttcctggac aagcatcaga 481 tgaaacacaa gaagctgccc ctgggcttca ccttctcctt tcctgtgagg cacgaagaca 541 tcgataaggg catccttctc aactggacca agggcttcaa ggcctcagga gcagaaggga 601 acaatgtcgt ggggcttctg cgagacgcta tcaaacggag aggggacttt gaaatggatg 661 tggtggcaat ggtgaatgac acggtggcca cgatgatctc ctgctactac gaagaccatc 721 agtgcgaggt cggcatgatc gtgggcacgg gctgcaatgc ctgctacatg gaggagatgc 781 agaatgtgga gctggtggag ggggacgagg gccgcatgtg cgtcaatacc gagtggggcg 841 ccttcgggga ctccggcgag ctggacgagt tcctgctgga gtatgaccgc ctggtggacg 901 agagctctgc aaaccccggt cagcagctgt atgagaagct cataggtggc aagtacatgg 961 gcgagctggt gcggcttgtg ctgctcaggc tcgtggacga aaacctgctc ttccacgggg 1021 aggCCTCCGA GCAGCTGCGC ACACGCGGAG CCTTCGAGAC GCGCTTCGTG TCGCAGGTGG 1081 AGAGCGACAC GGGCGACCGC AAGCAGATCT ACAACATCCT GAGCACGCTG GGGCTGCGAC 1141 CCTCGACCAC CGACTGCGAC ATCGTGCGCC GCGCCTGCGA GAGCGTGTCT ACGCGCGCTG 1201 CGCACATGTG CTCGGCGGGG CTGGCGGGCG TCATCAACCG CATGCGCGAG AGCCGCAGCG 1261 AGGACGTAAT GCGCATCACT GTGGGCGTGG ATGGCTCCGT GTACAAGCTG CACCCCAGCT 1321 TCAAGGAGCG GTTCCATGCC AGCGTGCGCA GGCTGACGCC CAGCTGCGAG ATCACCTTCA 1381 TCGAGTCGGA GGAGGGCAGT GGCCGGGGCG CGGCCCTGGT CTCGGCGGTG GCCTGTAAGA 1441 AGGCCTGTAT GCTGGGCCAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1501 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1561 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCGA TAACTTATGC 1621 TACTCCATAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt