Transcript: Human NM_001265605.1

Homo sapiens mortality factor 4 like 1 (MORF4L1), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Homo sapiens (human)
Gene:
MORF4L1 (10933)
Length:
1747
CDS:
355..1062

Additional Resources:

NCBI RefSeq record:
NM_001265605.1
NBCI Gene record:
MORF4L1 (10933)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001265605.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000107582 GTTGCCATAAAGGACAAACAA pLKO.1 187 5UTR 100% 5.625 7.875 N MORF4L1 n/a
2 TRCN0000239891 CATGAACAGAGTTGAAGTTAA pLKO_005 540 CDS 100% 13.200 9.240 N Morf4l1b n/a
3 TRCN0000107584 CGGAGAGCAGAGTACTCAAAT pLKO.1 263 5UTR 100% 13.200 9.240 N MORF4L1 n/a
4 TRCN0000300138 CGGAGAGCAGAGTACTCAAAT pLKO_005 263 5UTR 100% 13.200 9.240 N MORF4L1 n/a
5 TRCN0000107583 GTGTGTAAAGGTTGCCATAAA pLKO.1 177 5UTR 100% 13.200 9.240 N MORF4L1 n/a
6 TRCN0000300199 GTGTGTAAAGGTTGCCATAAA pLKO_005 177 5UTR 100% 13.200 9.240 N MORF4L1 n/a
7 TRCN0000107581 GCTGAAATTCTTGCAGATCAT pLKO.1 817 CDS 100% 4.950 3.465 N MORF4L1 n/a
8 TRCN0000300200 GCTGAAATTCTTGCAGATCAT pLKO_005 817 CDS 100% 4.950 3.465 N MORF4L1 n/a
9 TRCN0000107580 GCTTTGAAGATGTTAGTGTAT pLKO.1 1141 3UTR 100% 4.950 3.465 N MORF4L1 n/a
10 TRCN0000310570 GCTTTGAAGATGTTAGTGTAT pLKO_005 1141 3UTR 100% 4.950 3.465 N MORF4L1 n/a
11 TRCN0000015007 GCTTGTTGATGACTGGGACTT pLKO.1 591 CDS 100% 4.050 2.430 N MORF4 n/a
12 TRCN0000071526 CCTGCCAAGAAGAATGTGGAT pLKO.1 643 CDS 100% 2.640 1.584 N Morf4l1 n/a
13 TRCN0000238603 GGTGTATGGAGCGCCACATTT pLKO_005 858 CDS 100% 13.200 18.480 N Gm4835 n/a
14 TRCN0000218744 GAAACAGCGAGAACTTCAATA pLKO_005 303 5UTR 100% 13.200 9.240 N EG546908 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001265605.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14059 pDONR223 100% 99.8% 99.5% None 7G>T n/a
2 ccsbBroad304_14059 pLX_304 0% 99.8% 99.5% V5 7G>T n/a
3 ccsbBroadEn_15732 pDONR223 0% 99.8% 99.5% None 7G>T n/a
4 ccsbBroad304_15732 pLX_304 0% 99.8% 99.5% V5 7G>T n/a
5 TRCN0000472961 ACCCGGTGTCATGCCACGCTAAAA pLX_317 76.3% 99.8% 99.5% V5 7G>T n/a
6 ccsbBroadEn_02566 pDONR223 100% 72.7% 72.7% None 0_1ins264 n/a
7 ccsbBroad304_02566 pLX_304 0% 72.7% 72.7% V5 0_1ins264 n/a
8 TRCN0000468389 GCTCCCCGGTCGGACCGAAGGCAC pLX_317 38.5% 72.7% 72.7% V5 0_1ins264 n/a
9 ccsbBroadEn_02219 pDONR223 100% 68.3% 71.1% None (many diffs) n/a
10 ccsbBroad304_02219 pLX_304 0% 68.3% 71.1% V5 (many diffs) n/a
11 TRCN0000473386 TACGACTGAGGTCCGCGTAAGGTC pLX_317 65.6% 68.3% 71.1% V5 (many diffs) n/a
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