Construct: ORF TRCN0000468435
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016606.1_s317c1
- Derived from:
- ccsbBroadEn_09805
- DNA Barcode:
- GTGTACATTTACATTTGTTTCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3F (200316)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468435
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | NM_145298.6 | 99.7% | 99.4% | 322G>T;429C>T;691G>A |
| 2 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452179.1 | 99.7% | 99.4% | 322G>T;429C>T;691G>A |
| 3 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_152426.4 | 86.9% | 76.9% | (many diffs) |
| 4 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_024452178.1 | 84.1% | 83.9% | (many diffs) |
| 5 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XM_017028596.2 | 73.7% | 65.2% | (many diffs) |
| 6 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_017028642.2 | 55.5% | 52.9% | (many diffs) |
| 7 | human | 27350 | APOBEC3C | apolipoprotein B mRNA editi... | NM_014508.3 | 45.8% | 39.1% | (many diffs) |
| 8 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | NM_001363781.1 | 43.2% | 36.1% | (many diffs) |
| 9 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755170.2 | 35.2% | (many diffs) | |
| 10 | human | 140564 | APOBEC3D | apolipoprotein B mRNA editi... | XR_001755169.2 | 32.4% | (many diffs) | |
| 11 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | NM_001006666.2 | 23.2% | 17.8% | (many diffs) |
| 12 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XM_017028643.2 | 23.2% | 17.8% | (many diffs) |
| 13 | human | 200316 | APOBEC3F | apolipoprotein B mRNA editi... | XR_002958675.1 | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcctcacttc agaaacacag tggagcgaat gtatcgagac acattctcct 121 acaactttta taatagaccc atcctttctc gtcggaatac cgtctggctg tgctacgaag 181 tgaaaacaaa gggtccctca aggccccgtt tggacgcaaa gatctttcga ggccaggtgt 241 attcccagcc tgagcaccac gcagaaatgt gcttcctctc ttggttctgt ggcaaccagc 301 tgcctgctta caagtgtttc cagatcacct ggtttgtatc ctggaccccc tgcccggact 361 gtgtggcgaa gctggccgaa ttcctgtctg agcaccccaa tgtcaccctg accatctccg 421 ccgcccgcct ctactactac tgggaaagag attaccgaag ggcgctctgc aggctgagtc 481 aggcaggggc ccgtgtgaag attatggacg atgaagaatt tgcatactgc tgggaaaact 541 ttgtgtacag tgaaggtcag ccattcatgc cttggtacaa attcgatgac aattatgcat 601 tcctgcaccg cacgctaaag gagattctca gaaacccgat ggaggcaatg tatccacaca 661 tattctactt ccactttaaa aacctacgca aagcctatgg tcggaacgaa agctggctgt 721 gcttcaccat ggaagttgta aagcaccact cacctatctc ctggaagagg ggcgtcttcc 781 gaaaccaggt ggatccTGAG ACCCATTGTC ATGCAGAAAG GTGCTTCCTC TCTTGGTTCT 841 GTGACGACAT ACTGTCTCCT AACACAAACT ACGAGGTCAC CTGGTACACA TCTTGGAGCC 901 CTTGCCCAGA GTGTGCAGGG GAGGTGGCCG AGTTCCTGGC CAGGCACAGC AACGTGAATC 961 TCACCATCTT CACCGCCCGC CTCTACTACT TCTGGGATAC AGATTACCAG GAGGGGCTCC 1021 GCAGCCTGAG TCAGGAAGGG GCCTCCGTGG AGATCATGGG CTACAAAGAT TTTAAATATT 1081 GTTGGGAAAA CTTTGTGTAC AATGATGATG AGCCATTCAA GCCTTGGAAA GGACTAAAAT 1141 ACAACTTTCT ATTCCTGGAC AGCAAGCTGC AGGAGATTCT CGAGTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA GTGTACATTT ACATTTGTTT CTTAACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt