Construct: ORF TRCN0000468541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009524.1_s317c1
- Derived from:
- ccsbBroadEn_11435
- DNA Barcode:
- GTACTCTCGCCCATACTTCCATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOG (9941)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9941 | EXOG | exo/endonuclease G | XM_017007584.1 | 100% | 100% | |
| 2 | human | 9941 | EXOG | exo/endonuclease G | XM_017007585.1 | 100% | 100% | |
| 3 | human | 9941 | EXOG | exo/endonuclease G | XM_017007586.1 | 100% | 100% | |
| 4 | human | 9941 | EXOG | exo/endonuclease G | XM_017007587.1 | 100% | 100% | |
| 5 | human | 9941 | EXOG | exo/endonuclease G | XM_017007588.1 | 100% | 100% | |
| 6 | human | 9941 | EXOG | exo/endonuclease G | XM_017007589.1 | 100% | 100% | |
| 7 | human | 9941 | EXOG | exo/endonuclease G | XM_017007590.1 | 100% | 100% | |
| 8 | human | 9941 | EXOG | exo/endonuclease G | XM_017007591.1 | 100% | 100% | |
| 9 | human | 9941 | EXOG | exo/endonuclease G | XM_017007592.1 | 100% | 100% | |
| 10 | human | 9941 | EXOG | exo/endonuclease G | XM_017007593.1 | 100% | 100% | |
| 11 | human | 9941 | EXOG | exo/endonuclease G | XM_024453847.1 | 100% | 100% | |
| 12 | human | 9941 | EXOG | exo/endonuclease G | XM_024453848.1 | 100% | 100% | |
| 13 | human | 9941 | EXOG | exo/endonuclease G | XM_024453849.1 | 100% | 100% | |
| 14 | human | 9941 | EXOG | exo/endonuclease G | XM_006713442.3 | 86% | 86% | 1_111del |
| 15 | human | 9941 | EXOG | exo/endonuclease G | XM_017007594.2 | 76.5% | 69.4% | (many diffs) |
| 16 | human | 9941 | EXOG | exo/endonuclease G | NM_001145464.1 | 71.6% | 71.6% | 1_270del |
| 17 | human | 9941 | EXOG | exo/endonuclease G | NM_005107.4 | 61.9% | 61.9% | 1_420del |
| 18 | human | 9941 | EXOG | exo/endonuclease G | XR_940529.3 | 33.6% | 1_465del;691_809del;1269_2034del | |
| 19 | human | 9941 | EXOG | exo/endonuclease G | XR_940530.3 | 31.8% | 1_465del;691_925del;1385_2150del | |
| 20 | human | 9941 | EXOG | exo/endonuclease G | XR_940528.1 | 24.7% | 1_465del;691_806del;894_895ins371 | |
| 21 | human | 9941 | EXOG | exo/endonuclease G | NR_134938.2 | 23.5% | 1_354del;580_698del;1158_2906del | |
| 22 | human | 9941 | EXOG | exo/endonuclease G | NR_153322.1 | 22.4% | 1_422del;1107_3052del | |
| 23 | human | 9941 | EXOG | exo/endonuclease G | NR_153324.1 | 22.3% | 1_427del;1112_3057del | |
| 24 | human | 9941 | EXOG | exo/endonuclease G | XM_005265635.3 | 22.2% | 20.9% | (many diffs) |
| 25 | human | 9941 | EXOG | exo/endonuclease G | NR_153325.1 | 21.9% | 1_486del;1171_3116del | |
| 26 | human | 9941 | EXOG | exo/endonuclease G | NR_153327.1 | 21.2% | 1_583del;1268_3213del | |
| 27 | human | 9941 | EXOG | exo/endonuclease G | NR_153326.1 | 21.2% | 1_584del;1269_3214del | |
| 28 | human | 9941 | EXOG | exo/endonuclease G | NR_153329.1 | 20.9% | 1_640del;1325_3270del | |
| 29 | human | 9941 | EXOG | exo/endonuclease G | NR_153330.1 | 20.6% | 1_678del;1363_3308del | |
| 30 | human | 9941 | EXOG | exo/endonuclease G | NR_153328.1 | 20.4% | 1_722del;1407_3352del | |
| 31 | human | 9941 | EXOG | exo/endonuclease G | NR_153323.1 | 18.9% | 1_516del;739_1211del;1674_3619del | |
| 32 | mouse | 208194 | Exog | endo/exonuclease (5'-3'), e... | XM_011242946.2 | 83.5% | 85% | (many diffs) |
| 33 | mouse | 208194 | Exog | endo/exonuclease (5'-3'), e... | XM_011242947.2 | 83.5% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 753
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctccagca ggaaataaca aattttcaag taaagccatg gctgaaacct 121 tttacctttc taacattgtg cctcaggatt ttgataataa ttctggatat tggaacagaa 181 tagaaatgta ctgtcgagag ctgacagaaa ggtttgaaga tgtttgggtg gtatctgggc 241 ctttgacctt acctcagact agaggcgatg gaaagaaaat agttagttac caggtgattg 301 gcgaggacaa cgtggcagtc ccctcacacc tttataagGT AATCCTGGCC CGCAGAAGCT 361 CAGTATCTAC CGAACCACTG GCGCTAGGGG CCTTTGTGGT ACCCAATGAA GCCATCGGCT 421 TCCAGCCCCA GTTAACTGAA TTCCAAGTGA GCCTCCAGGA CCTAGAGAAG TTGTCAGGAC 481 TGGTGTTTTT TCCTCATTTG GATAGAACTA GTGATATCCG GAATATCTGC TCTGTGGACA 541 CCTGTAAGCT CCTGGATTTC CAGGAGTTCA CCTTGTACTT GAGTACAAGA AAGATTGAAG 601 GAGCCCGATC AGTGCTCAGA CTGGAAAAGA TCATGGAAAA CTTGAAGAAT GCAGAGATTG 661 AACCAGATGA TTACTTTATG AGTCGCTATG AGAAGAAGCT AGAAGAACTC AAAGCTAAGG 721 AGCAGTCAGG AACCCAGATA AGAAAGCCAT CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 901 ACTCTCGCCC ATACTTCCAT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt