Construct: ORF TRCN0000468604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017099.1_s317c1
- Derived from:
- ccsbBroadEn_12583
- DNA Barcode:
- ATCATTTCAATCTCAATGCATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHBDF2 (79651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | NM_001005498.3 | 74.8% | 74.8% | 1_624del |
| 2 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XM_005257670.1 | 74.8% | 74.8% | 1_624del |
| 3 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XM_011525249.2 | 74.8% | 74.8% | 1_624del |
| 4 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XM_011525250.2 | 74.8% | 74.8% | 1_624del |
| 5 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XM_011525251.2 | 74.8% | 74.8% | 1_624del |
| 6 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XM_017025079.1 | 74.8% | 74.8% | 1_624del |
| 7 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | NM_024599.5 | 72.3% | 72.3% | 1_711del |
| 8 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XM_005257669.3 | 72.3% | 72.3% | 1_711del |
| 9 | human | 79651 | RHBDF2 | rhomboid 5 homolog 2 | XR_001752625.2 | 50.6% | 1_998del;1676_1745del;2926_3665del | |
| 10 | mouse | 217344 | Rhbdf2 | rhomboid 5 homolog 2 | XM_017314494.1 | 85% | 85.3% | (many diffs) |
| 11 | mouse | 217344 | Rhbdf2 | rhomboid 5 homolog 2 | NM_001167680.1 | 66.1% | 69.4% | (many diffs) |
| 12 | mouse | 217344 | Rhbdf2 | rhomboid 5 homolog 2 | NM_172572.3 | 66.1% | 69.4% | (many diffs) |
| 13 | mouse | 217344 | Rhbdf2 | rhomboid 5 homolog 2 | XM_006533108.3 | 50.8% | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1923
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtggcccac atgagcttgc aagctgccgc tgccctcctc aaggggcgct 121 cggtgctgga tgccaccgga cagcggtgcc gggtggtcaa gcgcagcttt gccttcccga 181 gcttcctgga ggaggatgtg gtcgatgggg cagacacgtt tgactcctcc ttttttagta 241 aggaagaaat gagctccatg cctgatgatg tctttgagtc ccccccactc tctgccagct 301 acttccgagg gatcccacac tcagcctccc ctgtctcccc cgatggggtg caaatccctc 361 tgaaggagta tggccgagcc ccagtccccg ggccccggcg cggcaagcgc atcgcctcca 421 aggtgaagca ctttgccttt gatcggaaga agcggcacta cggcctcggc gtggtgggca 481 actggctgaa ccgcagctac cgccgcagca tcagcagcac tgtgcagcgg cagctggaga 541 gcttcgacag ccaccggccc tacttcacct actggctgac cttcgtccat gtcatcatca 601 cgctgctggt gatttgcacg tatggcatcg cacccgtggg ctttgcccag cacgtcacca 661 cccagctggt gctgcggaac aaaggtgtgt acgagagcgt gaagtacatc cagcaggaga 721 acttctgggt tggccccagc tcgattgacc tgatccacct gggggccaag ttctcaccct 781 gcatccggaa ggacgggcag atcgagcagc tggtgctgcg cgagcgagac ctggagcggg 841 actcaggctg ctgtgtccag aatgaccact ccggatgcat ccagacccag cggaaggact 901 gctcggagac tttggccact tttgtcaagt ggcaggatga cactgggccc cccatggaca 961 agtctgatct gggccagaag cggacttcgg gggctgtctg ccaccaggac cccaggacct 1021 gcgaggagcc agcctccagc ggtgcccaca tctggcccga tgacatcact aagtggccga 1081 tctgcacaga gcaggccagg agcaaccaca caggcttcct gcacatggac tgcgagatca 1141 agggccgccc ctgctgcatc ggcaccaagg gcagctgtga gatcaccacc cgggaatact 1201 gtgagttcat gcacggctat ttccatgagg aagcaacact ctgctcccag gtgcactgct 1261 tggacaaggt gtgtgggctg ctgcccttcc tcaaccctga ggtcccagat cagttctaca 1321 ggctctggct gtctctcttc ctacatgctg gcgtggtgca ctgcctcgtg tctgtggtct 1381 ttcaaatgac catcctgagg gacctggaga agctggccgg ctggcaccgt atcgccatca 1441 tcttcatcct cagtggcatc acaggcaacc tcgccagtgc catctttctc ccataccggg 1501 cagaggtggg cccggccggc tcacagttcg gcctcctcgc ctgccTCTTC GTGGAGCTCT 1561 TCCAGAGCTG GCCGCTGCTG GAGAGGCCCT GGAAGGCCTT CCTCAACCTC TCGGCCATCG 1621 TGCTCTTCCT GTTCATCTGT GGCCTCCTGC CCTGGATCGA CAACATCGCC CACATCTTCG 1681 GCTTCCTCAG TGGCCTGCTG CTGGCCTTCG CCTTCCTGCC CTACATCACC TTCGGCACCA 1741 GCGACAAGTA CCGCAAGCGG GCACTCATCC TGGTGTCACT GCTGGCCTTT GCCGGCCTCT 1801 TCGCCGCCCT CGTGCTGTGG CTGTACATCT ACCCCATTAA CTGGCCCTGG ATCGAGCACC 1861 TCACCTGCTT CCCCTTCACC AGCCGCTTCT GCGAGAAGTA TGAGCTGGAC CAGGTGCTGC 1921 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGAAT CATTTCAATC TCAATGCATT GTACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt