Construct: ORF TRCN0000468729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014802.1_s317c1
- Derived from:
- ccsbBroadEn_01039
- DNA Barcode:
- CAAAATATTAAAACTGATCGTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1B (4490)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 100% | 100% | |
| 2 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 91.2% | 91.8% | (many diffs) |
| 3 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 90.7% | 85.2% | (many diffs) |
| 4 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 90.1% | 85.2% | (many diffs) |
| 5 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 89.6% | 86.8% | (many diffs) |
| 6 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 89.6% | 86.8% | (many diffs) |
| 7 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 89% | 85.2% | (many diffs) |
| 8 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 88.5% | 85.2% | (many diffs) |
| 9 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 88.5% | 85.2% | (many diffs) |
| 10 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 88.1% | 83.8% | (many diffs) |
| 11 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 87.9% | 83.6% | (many diffs) |
| 12 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 39.9% | (many diffs) | |
| 13 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 37.2% | (many diffs) | |
| 14 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 35.6% | (many diffs) | |
| 15 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 32.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 216
- ORF end:
- 399
- ORF length:
- 183
- Sequence:
-
1 GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG 61 GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT 121 CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGCTAGCA 181 TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGGA TCCCAACTGC TCCTGCACCA 241 CAGGTGGCTC CTGTGCCTGC GCCGGCTCCT GCAAGTGCAA AGAGTGCAAA TGTACCTCCT 301 GCAAGAAGTG CTGCTGCTCT TGCTGCCCCG TGGGCTGTGC CAAGTGTGCC CAGGGCTGTG 361 TCTGCAAAGG CTCATCAGAG AAGTGCCGCT GCTGTGCCTG CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGACAAAAT ATTAAAACTG ATCGTATTAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt