Transcript: Human NM_005949.4

Homo sapiens metallothionein 1F (MT1F), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
MT1F (4494)
Length:
425
CDS:
101..286

Additional Resources:

NCBI RefSeq record:
NM_005949.4
NBCI Gene record:
MT1F (4494)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005949.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000072608 ACAACCTTTCTCCCAGATGTA pLKO.1 294 3UTR 100% 4.950 6.930 N MT1F n/a
2 TRCN0000072612 CGCTGGTTCCTGCAAGTGCAA pLKO.1 145 CDS 100% 0.880 0.616 N MT1F n/a
3 TRCN0000072610 GTAGCAAGTGTGCCCAGGGCT pLKO.1 222 CDS 100% 0.000 0.000 N MT1F n/a
4 TRCN0000242867 CAAGTGCAAAGAGTGCAAATG pLKO_005 157 CDS 100% 10.800 5.400 Y MT1G n/a
5 TRCN0000072609 GCAAGTGCAAAGAGTGCAAAT pLKO.1 156 CDS 100% 10.800 5.400 Y MT1F n/a
6 TRCN0000153590 GCAAGTGCAAAGAGTGCAAAT pLKO.1 156 CDS 100% 10.800 5.400 Y MT1E n/a
7 TRCN0000072611 CAAATGCACCTCCTGCAAGAA pLKO.1 172 CDS 100% 4.950 2.475 Y MT1F n/a
8 TRCN0000155121 CAAATGCACCTCCTGCAAGAA pLKO.1 172 CDS 100% 4.950 2.475 Y MT1X n/a
9 TRCN0000440406 ATGCACCTCCTGCAAGAAGAG pLKO_005 175 CDS 100% 4.050 2.025 Y MT1A n/a
10 TRCN0000242660 GCAAATGCACCTCCTGCAAGA pLKO_005 171 CDS 100% 4.050 2.025 Y MT1H n/a
11 TRCN0000364944 TGCAAATGCACCTCCTGCAAG pLKO_005 170 CDS 100% 4.050 2.025 Y MT1E n/a
12 TRCN0000156365 CTGCAAGTGCAAAGAGTGCAA pLKO.1 154 CDS 100% 2.640 1.320 Y MT1E n/a
13 TRCN0000155298 GCAAAGAGTGCAAATGCACCT pLKO.1 162 CDS 100% 2.160 1.080 Y MT1X n/a
14 TRCN0000242865 CCTGCAAGAAGAGCTGCTGCT pLKO_005 183 CDS 100% 0.720 0.360 Y MT1G n/a
15 TRCN0000219454 CTGCAAGAAGAGCTGCTGCTC pLKO.1 184 CDS 100% 0.720 0.360 Y Mt1 n/a
16 TRCN0000242659 AGAAGAGCTGCTGCTCCTGTT pLKO_005 189 CDS 100% 4.050 2.025 Y MT1H n/a
17 TRCN0000364945 CCTGCAAGAAGAGCTGCTGTT pLKO_005 183 CDS 100% 4.050 2.025 Y MT1E n/a
18 TRCN0000148975 GCAAAGAGTGCAAATGCACTT pLKO.1 162 CDS 100% 0.405 0.203 Y MT2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005949.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01041 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01041 pLX_304 95.5% 100% 100% V5 n/a
3 ccsbBroadEn_01040 pDONR223 100% 91.8% 93.4% None (many diffs) n/a
4 ccsbBroad304_01040 pLX_304 0% 91.8% 93.4% V5 (many diffs) n/a
5 TRCN0000465872 GTAGAGTCACTGCCTTGTCCTAGG pLX_317 100% 91.8% 93.4% V5 (many diffs) n/a
6 ccsbBroadEn_06598 pDONR223 100% 91.2% 85.2% None (many diffs) n/a
7 ccsbBroad304_06598 pLX_304 0% 91.2% 85.2% V5 (many diffs) n/a
8 TRCN0000473594 TGCAAGCTAGAGCAGCCGCGTATG pLX_317 100% 91.2% 85.2% V5 (many diffs) n/a
9 ccsbBroadEn_01045 pDONR223 100% 91.2% 91.8% None (many diffs) n/a
10 ccsbBroad304_01045 pLX_304 0% 91.2% 91.8% V5 (many diffs) n/a
11 TRCN0000472152 TGCCGATATAGCTCTCTATACTCA pLX_317 100% 91.2% 91.8% V5 (many diffs) n/a
12 ccsbBroadEn_01042 pDONR223 100% 91.2% 83.6% None (many diffs) n/a
13 ccsbBroad304_01042 pLX_304 0% 91.2% 83.6% V5 (many diffs) n/a
14 TRCN0000470527 TTTTTAACCATTGCTGGCACAAAG pLX_317 100% 91.2% 83.6% V5 (many diffs) n/a
15 ccsbBroadEn_06599 pDONR223 100% 90.7% 86.8% None (many diffs) n/a
16 ccsbBroad304_06599 pLX_304 0% 90.7% 86.8% V5 (many diffs) n/a
17 TRCN0000474764 TTGATAACTTTGGCATCACCATAC pLX_317 100% 90.7% 86.8% V5 (many diffs) n/a
18 ccsbBroadEn_01044 pDONR223 100% 88.5% 83.6% None (many diffs) n/a
19 ccsbBroad304_01044 pLX_304 0% 88.5% 83.6% V5 (many diffs) n/a
20 TRCN0000475214 ATCGACCACCTTCTCGGATCAACG pLX_317 100% 88.5% 83.6% V5 (many diffs) n/a
21 ccsbBroadEn_13207 pDONR223 100% 88.5% 85.2% None (many diffs) n/a
22 ccsbBroad304_13207 pLX_304 0% 88.5% 85.2% V5 (many diffs) n/a
23 TRCN0000467056 AGATTTTCATGCAACTTTTCTCGA pLX_317 100% 88.5% 85.2% V5 (many diffs) n/a
24 ccsbBroadEn_01039 pDONR223 100% 88.5% 85.2% None (many diffs) n/a
25 ccsbBroad304_01039 pLX_304 0% 88.5% 85.2% V5 (many diffs) n/a
26 TRCN0000468729 CAAAATATTAAAACTGATCGTATT pLX_317 100% 88.5% 85.2% V5 (many diffs) n/a
27 ccsbBroadEn_01043 pDONR223 100% 88.5% 83.6% None (many diffs) n/a
28 ccsbBroad304_01043 pLX_304 0% 88.5% 83.6% V5 (many diffs) n/a
29 ccsbBroadEn_10353 pDONR223 100% 71% 63.9% None (many diffs) n/a
30 ccsbBroad304_10353 pLX_304 0% 71% 63.9% V5 (many diffs) n/a
31 TRCN0000473861 CGCCCGCAGATCATTCGATACGCC pLX_317 100% 71% 63.9% V5 (many diffs) n/a
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