Transcript: Human NM_005953.5

Homo sapiens metallothionein 2A (MT2A), mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
MT2A (4502)
Length:
401
CDS:
73..258

Additional Resources:

NCBI RefSeq record:
NM_005953.5
NBCI Gene record:
MT2A (4502)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005953.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000147402 GCAAATGCAAAGAGTGCAAAT pLKO.1 128 CDS 100% 10.800 5.400 Y MT2A n/a
2 TRCN0000150838 GCAAATGCAAAGAGTGCAAAT pLKO.1 128 CDS 100% 10.800 5.400 Y MT1X n/a
3 TRCN0000159156 GCAAATGCAAAGAGTGCAAAT pLKO.1 128 CDS 100% 10.800 5.400 Y MT1A n/a
4 TRCN0000072611 CAAATGCACCTCCTGCAAGAA pLKO.1 144 CDS 100% 4.950 2.475 Y MT1F n/a
5 TRCN0000155121 CAAATGCACCTCCTGCAAGAA pLKO.1 144 CDS 100% 4.950 2.475 Y MT1X n/a
6 TRCN0000162839 CTCCTGCAAATGCAAAGAGTG pLKO.1 123 CDS 100% 4.050 2.025 Y MT1A n/a
7 TRCN0000242660 GCAAATGCACCTCCTGCAAGA pLKO_005 143 CDS 100% 4.050 2.025 Y MT1H n/a
8 TRCN0000364944 TGCAAATGCACCTCCTGCAAG pLKO_005 142 CDS 100% 4.050 2.025 Y MT1E n/a
9 TRCN0000148783 CTGCAAATGCAAAGAGTGCAA pLKO.1 126 CDS 100% 2.640 1.320 Y MT2A n/a
10 TRCN0000155298 GCAAAGAGTGCAAATGCACCT pLKO.1 134 CDS 100% 2.160 1.080 Y MT1X n/a
11 TRCN0000242658 CCCAGGGCTGCATCTGCAAAG pLKO_005 206 CDS 100% 0.000 0.000 Y MT1H n/a
12 TRCN0000154166 CTCCTGCAAATGCAAAGAGTA pLKO.1 123 CDS 100% 4.950 2.475 Y MT1DP n/a
13 TRCN0000148975 GCAAAGAGTGCAAATGCACTT pLKO.1 134 CDS 100% 0.405 0.203 Y MT2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005953.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01045 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01045 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472152 TGCCGATATAGCTCTCTATACTCA pLX_317 100% 100% 100% V5 n/a
4 ccsbBroadEn_01044 pDONR223 100% 92.9% 90.1% None (many diffs) n/a
5 ccsbBroad304_01044 pLX_304 0% 92.9% 90.1% V5 (many diffs) n/a
6 TRCN0000475214 ATCGACCACCTTCTCGGATCAACG pLX_317 100% 92.9% 90.1% V5 (many diffs) n/a
7 ccsbBroadEn_01043 pDONR223 100% 92.9% 90.1% None (many diffs) n/a
8 ccsbBroad304_01043 pLX_304 0% 92.9% 90.1% V5 (many diffs) n/a
9 ccsbBroadEn_01040 pDONR223 100% 92.3% 93.4% None (many diffs) n/a
10 ccsbBroad304_01040 pLX_304 0% 92.3% 93.4% V5 (many diffs) n/a
11 TRCN0000465872 GTAGAGTCACTGCCTTGTCCTAGG pLX_317 100% 92.3% 93.4% V5 (many diffs) n/a
12 ccsbBroadEn_06598 pDONR223 100% 91.8% 88.5% None (many diffs) n/a
13 ccsbBroad304_06598 pLX_304 0% 91.8% 88.5% V5 (many diffs) n/a
14 TRCN0000473594 TGCAAGCTAGAGCAGCCGCGTATG pLX_317 100% 91.8% 88.5% V5 (many diffs) n/a
15 ccsbBroadEn_01041 pDONR223 100% 91.2% 91.8% None (many diffs) n/a
16 ccsbBroad304_01041 pLX_304 95.5% 91.2% 91.8% V5 (many diffs) n/a
17 ccsbBroadEn_06599 pDONR223 100% 91.2% 90.1% None (many diffs) n/a
18 ccsbBroad304_06599 pLX_304 0% 91.2% 90.1% V5 (many diffs) n/a
19 TRCN0000474764 TTGATAACTTTGGCATCACCATAC pLX_317 100% 91.2% 90.1% V5 (many diffs) n/a
20 ccsbBroadEn_01042 pDONR223 100% 90.1% 81.9% None (many diffs) n/a
21 ccsbBroad304_01042 pLX_304 0% 90.1% 81.9% V5 (many diffs) n/a
22 TRCN0000470527 TTTTTAACCATTGCTGGCACAAAG pLX_317 100% 90.1% 81.9% V5 (many diffs) n/a
23 ccsbBroadEn_01039 pDONR223 100% 88.5% 85.2% None (many diffs) n/a
24 ccsbBroad304_01039 pLX_304 0% 88.5% 85.2% V5 (many diffs) n/a
25 TRCN0000468729 CAAAATATTAAAACTGATCGTATT pLX_317 100% 88.5% 85.2% V5 (many diffs) n/a
26 ccsbBroadEn_13207 pDONR223 100% 87.4% 85.2% None (many diffs) n/a
27 ccsbBroad304_13207 pLX_304 0% 87.4% 85.2% V5 (many diffs) n/a
28 TRCN0000467056 AGATTTTCATGCAACTTTTCTCGA pLX_317 100% 87.4% 85.2% V5 (many diffs) n/a
29 ccsbBroadEn_10353 pDONR223 100% 72.1% 65.5% None (many diffs) n/a
30 ccsbBroad304_10353 pLX_304 0% 72.1% 65.5% V5 (many diffs) n/a
31 TRCN0000473861 CGCCCGCAGATCATTCGATACGCC pLX_317 100% 72.1% 65.5% V5 (many diffs) n/a
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