Construct: ORF TRCN0000468750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007210.1_s317c1
- Derived from:
- ccsbBroadEn_08101
- DNA Barcode:
- GCGGACCAATCTGGGAATACGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM230 (29058)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001009924.1 | 99.7% | 99.1% | 356A>C |
| 2 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001009925.1 | 99.7% | 99.1% | 356A>C |
| 3 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330984.1 | 99.7% | 99.1% | 356A>C |
| 4 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330985.1 | 99.7% | 99.1% | 356A>C |
| 5 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330986.1 | 99.7% | 99.1% | 356A>C |
| 6 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_014145.4 | 99.7% | 99.1% | 356A>C |
| 7 | human | 29058 | TMEM230 | transmembrane protein 230 | XM_017027836.2 | 99.7% | 99.1% | 356A>C |
| 8 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330987.1 | 66.4% | 56.9% | (many diffs) |
| 9 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001009923.2 | 65.3% | 65% | 1_189del;545A>C |
| 10 | human | 29058 | TMEM230 | transmembrane protein 230 | XM_024451879.1 | 46.3% | 40.1% | (many diffs) |
| 11 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958485.1 | 26% | (many diffs) | |
| 12 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958479.1 | 25.6% | (many diffs) | |
| 13 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958476.1 | 23.6% | (many diffs) | |
| 14 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958484.1 | 23.1% | (many diffs) | |
| 15 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958482.1 | 22.3% | (many diffs) | |
| 16 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958481.1 | 21.9% | (many diffs) | |
| 17 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958483.1 | 21.7% | (many diffs) | |
| 18 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958478.1 | 14.7% | (many diffs) | |
| 19 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958480.1 | 14.3% | (many diffs) | |
| 20 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958477.1 | 12.8% | (many diffs) | |
| 21 | mouse | 70612 | Tmem230 | transmembrane protein 230 | NM_001141971.1 | 90.8% | 95.8% | (many diffs) |
| 22 | mouse | 70612 | Tmem230 | transmembrane protein 230 | NM_027478.3 | 90.8% | 95.8% | (many diffs) |
| 23 | mouse | 70612 | Tmem230 | transmembrane protein 230 | XM_006500166.3 | 90.8% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 450
- ORF length:
- 360
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATGCCGTC CCGTACCAAC CTGGCTACTG 121 GAATCCCCAG TAGTAAAGTG AAATATTCAA GGCTCTCCAG CACAGACGAT GGCTACATTG 181 ACCTTCAGTT TAAGAAAACC CCTCCTAAGA TCCCTTATAA GGCCATCGCA CTTGCCACTG 241 TGCTGTTTTT GATTGGCGCC TTTCTCATTA TTATAGGCTC CCTCCTGCTG TCAGGCTACA 301 TCAGCAAAGG GGGGGCAGAC CGGGCCGTTC CAGTGCTGAT CATTGGCATT CTGGTGTTCC 361 TACCCGGATT TTACCACCTG CGCATCGCTT ACTATGCATC CAAAGGCTAC CGTGGTTACT 421 CCTATGATGA CATTCCAGAC TTTGCTGACT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCGGA 601 CCAATCTGGG AATACGTATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt