Construct: ORF TRCN0000468882
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001642.1_s317c1
- Derived from:
- ccsbBroadEn_08697
- DNA Barcode:
- TCTGTAAGCTCTGTTATACATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPLA2 (57104)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468882
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57104 | PNPLA2 | patatin like phospholipase ... | NM_020376.4 | 99.9% | 100% | 873C>G |
| 2 | human | 57104 | PNPLA2 | patatin like phospholipase ... | XM_017018028.1 | 99.9% | 100% | 873C>G |
| 3 | human | 57104 | PNPLA2 | patatin like phospholipase ... | XM_024448618.1 | 86.8% | 75.7% | 873C>G;919_1060del;1315_1400del |
| 4 | human | 57104 | PNPLA2 | patatin like phospholipase ... | XM_006718265.3 | 84.3% | 54% | 873C>G;919_1060del;1655_1791del |
| 5 | human | 57104 | PNPLA2 | patatin like phospholipase ... | XM_006718266.3 | 84.3% | 54% | 873C>G;919_1060del;1655_1791del |
| 6 | mouse | 66853 | Pnpla2 | patatin-like phospholipase ... | NM_001163689.1 | 80.3% | 83.5% | (many diffs) |
| 7 | mouse | 66853 | Pnpla2 | patatin-like phospholipase ... | NM_025802.3 | 73.2% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1578
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tccccgcgag aagacgtgga acatctcgtt cgcgggctgc ggcttcctcg 121 gcgtctacta cgtcggcgtg gcctcctgcc tccgcgagca cgcgcccttc ctggtggcca 181 acgccacgca catctacggc gcctcggccg gggcgctcac ggccacggcg ctggtcaccg 241 gggtctgcct gggtgaggct ggtgccaagt tcattgaggt atctaaagag gcccggaagc 301 ggttcctggg ccccctgcac ccctccttca acctggtaaa gatcatccgc agtttcctgc 361 tgaaggtcct gcctgctgat agccatgagc atgccagtgg gcgcctgggc atctccctga 421 cccgcgtgtc agacggcgag aatgtcatta tatcccactt caactccaag gacgagctca 481 tccaggccaa tgtctgcagc ggtttcatcc ccgtgtactg tgggctcatc cctccctccc 541 tccagggggt gcgctacgtg gatggtggca tttcagacaa cctgccactc tatgagctta 601 agaacaccat cacagtgtcc cccttctcgg gcgagagtga catctgtccg caggacagct 661 ccaccaacat ccacgagctg cgggtcacca acaccagcat ccagttcaac ctgcgcaacc 721 tctaccgcct ctccaaggcc ctcttcccgc cggagcccct ggtgctgcga gagatgtgca 781 agcagggata ccgggatggc ctgcgctttc tgcagcggaa cggcctcctg aaccggccca 841 accccttgct ggcgttgccc cccgcccgcc cccacggccc agaggacaag gaccaggcag 901 tggagagcgc ccaagcggag gattactcgc agctgccggg agaagatcac atcctggagc 961 acctgcccgc ccggctcaat gaggccctgc tggaggcctg cgtggagccc acggacctgc 1021 tgaccaccct ctccaacatg ctgcctgtgc gtctggccac ggccatgatg gtgccctaca 1081 cgctgccgct ggagagcgct ctgtccttca ccatccgctt gctggagtgg ctgcccGACG 1141 TTCCCGAGGA CATCCGGTGG ATGAAGGAGC AGACGGGCAG CATCTGCCAG TACCTGGTGA 1201 TGCGCGCCAA GAGGAAGCTG GGCAGGCACC TGCCCTCCAG GCTGCCGGAG CAGGTGGAGC 1261 TGCGCCGCGT CCAGTCGCTG CCGTCCGTGC CGCTGTCCTG CGCCGCCTAC AGAGAGGCAC 1321 TGCCCGGCTG GATGCGCAAC AACCTCTCGC TGGGGGACGC GCTGGCCAAG TGGGAGGAGT 1381 GCCAGCGCCA GCTGCTGCTC GGCCTCTTCT GCACCAACGT GGCCTTCCCG CCCGAAGCTC 1441 TGCGCATGCG CGCACCCGCC GACCCGGCTC CCGCCCCCGC GGACCCAGCA TCCCCGCAGC 1501 ACCAGCTGGC CGGGCCTGCC CCCTTGCTGA GCACCCCTGC TCCCGAGGCC CGGCCCGTGA 1561 TCGGGGCCCT GGGGCTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCTGTAA GCTCTGTTAT 1741 ACATTCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt