Construct: ORF TRCN0000468927
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010032.1_s317c1
- Derived from:
- ccsbBroadEn_09908
- DNA Barcode:
- GCAAGGCCTCGTTTGTGTGCGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF718 (255403)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468927
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255403 | ZNF718 | zinc finger protein 718 | NM_001039127.6 | 100% | 100% | |
| 2 | human | 255403 | ZNF718 | zinc finger protein 718 | NM_001289930.1 | 93.3% | 93.3% | 0_1ins96 |
| 3 | human | 255403 | ZNF718 | zinc finger protein 718 | NM_001289931.1 | 93.3% | 93.3% | 0_1ins96 |
| 4 | human | 255403 | ZNF718 | zinc finger protein 718 | XM_017007979.2 | 93.3% | 93.3% | 0_1ins96 |
| 5 | human | 255403 | ZNF718 | zinc finger protein 718 | XM_017007980.2 | 93.3% | 93.3% | 0_1ins96 |
| 6 | human | 152687 | ZNF595 | zinc finger protein 595 | NM_182524.4 | 63.1% | 54.6% | (many diffs) |
| 7 | human | 255403 | ZNF718 | zinc finger protein 718 | NR_110529.1 | 36.8% | (many diffs) | |
| 8 | human | 7700 | ZNF141 | zinc finger protein 141 | NM_001348278.2 | 36.1% | 30.2% | (many diffs) |
| 9 | human | 7700 | ZNF141 | zinc finger protein 141 | NM_001348280.2 | 16.6% | 14.2% | (many diffs) |
| 10 | human | 107986244 | LOC107986244 | zinc finger protein 141-like | XM_017008889.1 | 14.1% | 12% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1503
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaactctta acattcaagg atgtggccat agaattctct ccagaagagt 121 ggaaatgtct ggacacttcc cagcagaatt tatatagaga tgtgatgttg gagaactaca 181 gaaacctggt ctccctgggt gttagtatct ctaacccaga cctggtcacc agtctggagc 241 aaagaaaaga gccctacaat ttgaagatac atgaaacagc agccagaccc ccagctgtgt 301 gttctcattt cacccaaaac ctttggacag tgcagggcat agaagattca ttccacaaac 361 ttataccaaa aggacatgag aaacgtggac atgagaattt aagaaaaact tgtaaaagta 421 taaatgagtg taaggtgcag aaaggtggtt ataatagaat taaccaatgc ttattaacta 481 cccagaaaaa aacaattcaa tctaatatat gtgtcaaagt ttttcataaa ttttcaaatt 541 caaacaaaga taagataaga tatactggag ataaaacctt taaatgtaaa gaatgtggca 601 aatcatttca cgtgctctca cgcctaactc aacacaaaag aattcatact ggagagaacc 661 cctacacatg tgaagaatgt ggcaaagcct ttaattggtc ctcaattctt actaaacata 721 agagaattca tgccagagag aaattctaca agtgtgaaga atgtggtaaa ggctttactc 781 ggtcctcaca ccttactaaa cataagagaa ttcatactgg agagaaaccc tacatatgtg 841 aaaaatgtgg taaagctttt aaccaatcct caacccttaa tttacataag agaattcatt 901 ctgcacaaaa atactacaaa tgtgaagaat gtggtaaagc ctttaagtgg tcctcatccc 961 ttaatgaaca taagagaatt catgctggag agaaaccctt ctcatgcgaa gaatgtggca 1021 atgtctttac cacatcctca gactttgcta aacataagag aattcataca ggagagaaac 1081 cctacaaATG TGAAGAATGT GGAAAATCCT TTAATAGGTC CACAACTCTT ACGACACATA 1141 AGAGAATCCA TACTGGAGAG AAACCCTACA CATGTGAAGA ATGTGGAAAA GCCTTTAATT 1201 GGTCCTCAAC CCTTAATGTA CACAAGAGAA TTCACTCTGG AAAAAATCCC TACAAATGTG 1261 AAGATTGTGG CAAAGCCTTT AAAGTGTTTG CAAACCTGCA TAATCATAAG AAAATTCATA 1321 CTGGAGAGAA ACCCTACATA TGTAAACAAT GTGGCAAAGC CTTTAAACAG TCCTCACACT 1381 TGAATAAACA TAAGAAAATT CACACTGTAG ATAAACCCTA CAAATGTAAA GAATGCGGGA 1441 AAGCTTTTAA GCAGTACTCC AACCTTCCTC AACATAAGAG AACTCATACT GGAGGAAAAT 1501 TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGAGC AAGGCCTCGT TTGTGTGCGG AGACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt