Construct: ORF TRCN0000469052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000370.1_s317c1
- Derived from:
- ccsbBroadEn_15494
- DNA Barcode:
- TCAGTACTAGGAAGGTCTCTACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAX (4149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4149 | MAX | MYC associated factor X | NM_145112.3 | 99.7% | 99.3% | 382G>T |
| 2 | human | 4149 | MAX | MYC associated factor X | NM_002382.5 | 94.1% | 93.7% | 37_63del;409G>T |
| 3 | human | 4149 | MAX | MYC associated factor X | XM_011536773.3 | 69.7% | 48.7% | (many diffs) |
| 4 | human | 4149 | MAX | MYC associated factor X | NM_145113.3 | 57.9% | 55.6% | (many diffs) |
| 5 | human | 4149 | MAX | MYC associated factor X | NM_001320415.2 | 54.7% | 39.8% | (many diffs) |
| 6 | human | 4149 | MAX | MYC associated factor X | XM_017021312.2 | 54.7% | 39.8% | (many diffs) |
| 7 | human | 4149 | MAX | MYC associated factor X | XM_017021313.1 | 54.7% | 39.8% | (many diffs) |
| 8 | human | 4149 | MAX | MYC associated factor X | NM_001271069.1 | 44.5% | 32.4% | (many diffs) |
| 9 | human | 4149 | MAX | MYC associated factor X | NM_001271068.1 | 42.9% | 34.3% | (many diffs) |
| 10 | human | 4149 | MAX | MYC associated factor X | NM_197957.3 | 42% | 30.6% | (many diffs) |
| 11 | human | 4149 | MAX | MYC associated factor X | NM_145114.2 | 40.7% | 32.5% | (many diffs) |
| 12 | human | 4149 | MAX | MYC associated factor X | NR_073137.1 | 22.2% | (many diffs) | |
| 13 | human | 4149 | MAX | MYC associated factor X | XR_001750327.2 | 21.5% | (many diffs) | |
| 14 | human | 4149 | MAX | MYC associated factor X | XR_943452.3 | 20.9% | (many diffs) | |
| 15 | human | 4149 | MAX | MYC associated factor X | XR_001750326.2 | 20.7% | (many diffs) | |
| 16 | human | 4149 | MAX | MYC associated factor X | XR_943450.3 | 20.5% | (many diffs) | |
| 17 | human | 4149 | MAX | MYC associated factor X | XR_943451.3 | 20.3% | (many diffs) | |
| 18 | human | 4149 | MAX | MYC associated factor X | XR_002957553.1 | 17.3% | (many diffs) | |
| 19 | mouse | 17187 | Max | Max protein | NM_001146176.1 | 95.5% | 98.6% | (many diffs) |
| 20 | mouse | 17187 | Max | Max protein | NM_008558.2 | 90.2% | 93.1% | (many diffs) |
| 21 | mouse | 17187 | Max | Max protein | XM_006515523.3 | 76.4% | 75.4% | (many diffs) |
| 22 | mouse | 17187 | Max | Max protein | XM_006515524.3 | 56% | 55.6% | (many diffs) |
| 23 | mouse | 17187 | Max | Max protein | XM_017314968.1 | 39.2% | 40.5% | (many diffs) |
| 24 | mouse | 17187 | Max | Max protein | XR_001780418.1 | 20.4% | (many diffs) | |
| 25 | mouse | 17187 | Max | Max protein | XR_381483.3 | 19.8% | (many diffs) | |
| 26 | mouse | 17187 | Max | Max protein | XR_872708.2 | 19.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgataacgat gacatcgagg tggagagcga cgctgacaaa cgggctcatc 121 ataatgcact ggaacgaaaa cgtagggacc acatcaaaga cagctttcac agtttgcgGG 181 ACTCAGTCCC ATCACTCCAA GGAGAGAAGG CATCCCGGGC CCAAATCCTA GACAAAGCCA 241 CAGAATATAT CCAGTATATG CGAAGGAAAA ACCACACACA CCAGCAAGAT ATTGACGACC 301 TCAAGCGGCA GAATGCTCTT CTGGAGCAGC AAGTCCGTGC ACTGGAGAAG GCGAGGTCAA 361 GTGCCCAACT GCAGACCAAC TACCCCTCCT CAGACAACAG CCTCTACACC AACGCCAAGG 421 GCAGCACCAT CTCTGCCTTC GATGGGTGCT CGGACTCCAG CTCGGAGTCT GAGCCTGAAG 481 AGCCCCAAAG CAGGAAGAAG CTCCGGATGG AGGCCAGCTA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATCAGTA CTAGGAAGGT CTCTACGAAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt