Construct: ORF TRCN0000469342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018932.2_s317c1
- Derived from:
- ccsbBroadEn_14876
- DNA Barcode:
- AACACCAGCCCCCAACCGAACCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- VRK1 (7443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469342
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | NM_003384.3 | 99.9% | 52.2% | 619_620insA |
| 2 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021624.2 | 99.6% | 52.4% | 619_620insA;1065_1066insAAG |
| 3 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021625.1 | 99.4% | 52% | 1_6delATGAAA;625_626insA |
| 4 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_006720247.4 | 94.2% | 49.2% | 619_620insA;1160_1231del |
| 5 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_011537132.1 | 93.9% | 49.4% | 619_620insA;1065_1066insAAG;1157_1228del |
| 6 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XR_001750539.2 | 64% | (many diffs) | |
| 7 | human | 7443 | VRK1 | VRK serine/threonine kinase 1 | XM_017021626.2 | 61.5% | 84.8% | 619_620insA;708_709ins121;732_733ins335 |
| 8 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_001029844.1 | 84.4% | 47.9% | (many diffs) |
| 9 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_017315046.1 | 84.4% | 47.9% | (many diffs) |
| 10 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_001029843.1 | 80.7% | 45.6% | (many diffs) |
| 11 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_011244103.1 | 79.8% | 45.2% | (many diffs) |
| 12 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | NM_011705.3 | 76.3% | 43.1% | (many diffs) |
| 13 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515809.2 | 76.3% | 43.1% | (many diffs) |
| 14 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515810.3 | 76.3% | 43.1% | (many diffs) |
| 15 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515811.3 | 76.3% | 43.1% | (many diffs) |
| 16 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_006515812.2 | 76.3% | 43.1% | (many diffs) |
| 17 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_017315045.1 | 76.1% | 43.2% | (many diffs) |
| 18 | mouse | 22367 | Vrk1 | vaccinia related kinase 1 | XM_011244104.2 | 51.6% | 17.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 699
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcgtgta aaagcagctc aagctggaag acagagctct gcaaagagac 121 atcttgcaga acaatttgca gttggagaga taataactga catggcaaaa aaggaatgga 181 aagtaggatt acccattggc caaggaggct ttggctgtat atatcttgct gatatgaatt 241 cttcagagtc agttggcagt gatgcacctt gtgttgtaaa agtggaaccc agtgacaatg 301 gacctctttt tactgaatta aagttctacc aacgagctgc aaaaccagag caaattcaga 361 aatggattcg tacccgtaag ctgaagtacc tgggtgttcc taagtattgg gggtctggtc 421 tacatgacaa aaatggaaaa agttacaggt ttatgataat ggatcgcttt gggagtgacc 481 ttcagaaaat atatgaagca aatgccaaaa ggttttctcg gaaaactgtc ttgcagctaa 541 gcttaagaat tctggatatt ctggaatata ttcacgagca tgagtatgtg catggagata 601 tcaaggcctc aaatcttctt ctgaactaca agaatcctga ccaggtgtac ttggtagatt 661 atggccttgc ttatcggtac tgcccagaaa ggagttcata aagaatacaa agaagacccc 721 aaaagatgtc acgatggcac tattgaattc acgagcatcg atgcacacaa tggcgtggcc 781 ccatcaagac gtggtgattt ggaaatactt ggttattgca tgatccaatg gcttactggc 841 catcttcctt gggaggataa tttGAAAGAT CCTAAATATG TTAGAGATTC CAAAATTAGA 901 TACAGAGAAA ATATTGCAAG TTTGATGGAC AAATGTTTTC CTGAGAAAAA CAAACCAGGT 961 GAAATTGCCA AATACATGGA AACAGTGAAA TTACTAGACT ACACTGAAAA ACCTCTTTAT 1021 GAAAATTTAC GTGACATTCT TTTGCAAGGA CTAAAAGCTA TAGGAAGTAA GGATGATGGC 1081 AAATTGGACC TCAGTGTTGT GGAGAATGGA GGTTTGAAAG CAAAAACAAT AACAAAGAAG 1141 CGAAAGAAAG AAATTGAAGA AAGCAAGGAA CCTGGTGTTG AAGATACGGA ATGGTCAAAC 1201 ACACAGACAG AGGAGGCCAT ACAGACCCGT TCAAGAACCA GAAAGAGAGT CCAGAAGTTG 1261 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1321 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1381 TATATATCTT GTGGAAAGGA CGAAACACCA GCCCCCAACC GAACCCGACG CGTTAAGTCg 1441 acaatcaacc tctggattac aaaatttgtg aaagatt