Construct: ORF TRCN0000469520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012945.1_s317c1
- Derived from:
- ccsbBroadEn_08027
- DNA Barcode:
- ACGGAGTGAACTCCAGACTTATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR10H1 (26539)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26539 | OR10H1 | olfactory receptor family 1... | NM_013940.3 | 99.8% | 100% | 393C>T |
| 2 | human | 26539 | OR10H1 | olfactory receptor family 1... | XM_011527911.1 | 99.8% | 100% | 393C>T |
| 3 | human | 284433 | OR10H5 | olfactory receptor family 1... | NM_001004466.1 | 94.7% | 92.1% | (many diffs) |
| 4 | human | 26538 | OR10H2 | olfactory receptor family 1... | NM_013939.2 | 88.6% | 86.7% | (many diffs) |
| 5 | mouse | 100038860 | Olfr239 | olfactory receptor 239 | NM_207175.2 | 83.2% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1023
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagagagcc aatcactcca cagtgaccca attcatcctc gtcggcttct 121 ctgtcttccc ccacctccag ctgatgctct tcctgctgtt cctgctgatg tacctgttca 181 cgctgctggg caacctgctc atcatggcca ccgtctggag cgagcgcagc ctccacacgc 241 ccatgtacct cttcctgtgc gccctctccg tctccgagat cctctacacc gtggccatca 301 tcccgcgcat gctggccgac ctgctgtcca cccagcgctc catcgccttc ctggcctgtg 361 ccagtcagat gttcttctcc ttcagcttcg gcttcaccca ctccttcctg ctcaccgtca 421 tgggctacga ccgctacgtg gccatctgcc accccctgcg ttacaacgtg ctcatgagcc 481 cgcggggctg cgcctgcctg gtgggctgct cctgggctgg tggcttggtc atggggatgg 541 tggtgacctc ggccattttc cacctcgcct tctgtggaca caaggagatc caccattttg 601 cttgccatgt gccacctctg ttgaagttgg cctgtggaga cgatgtgctg gtggtggcca 661 aaggcgtggg cttggtgtgt atcacggccc tgctgggctg ttttctcctc atcctcctct 721 cctatgcctt catcgtggcc gccatcttga agatcccttc tgctgaaggt cggaacaagg 781 ccttctccac ctgtgcctct cacctcactg tggtggtcgt gcactatggc tttgccTCCG 841 TCATTTACCT GAAGCCCAAA AGTCCCCAGT CTCTGGAAGG AGACACCTTG ATGGGCATCA 901 CCTACACGGT CCTCACACCC TTCCTCAGCC CCATCATCTT CAGCCTCAGG AACAAGGAGC 961 TGAAGGTCGC CATGAAGAAG ACCTTCTTCA GTAAACTCTA CCCAGAAAAA AATGTAATGA 1021 TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GGAGTGAACT CCAGACTTAT CCACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt