Construct: ORF TRCN0000469640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014349.1_s317c1
- Derived from:
- ccsbBroadEn_06667
- DNA Barcode:
- GCATCCATCTACTCGCACACCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OCLN (100506658)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100506658 | OCLN | occludin | NM_001205254.2 | 99.8% | 99.8% | 96G>A;698T>C |
| 2 | human | 100506658 | OCLN | occludin | NM_002538.4 | 99.8% | 99.8% | 96G>A;698T>C |
| 3 | human | 100506658 | OCLN | occludin | XM_017008913.2 | 89.5% | 89.4% | 96G>A;698T>C;728_729ins162 |
| 4 | human | 100506658 | OCLN | occludin | XM_017008914.2 | 89.5% | 89.4% | 96G>A;698T>C;728_729ins162 |
| 5 | human | 100506658 | OCLN | occludin | NM_001205255.1 | 51.9% | 51.9% | 0_1ins753 |
| 6 | human | 647859 | OCLNP1 | OCLN pseudogene 1 | NR_026578.1 | 34.6% | (many diffs) | |
| 7 | mouse | 18260 | Ocln | occludin | NM_001360536.1 | 85.8% | 89.6% | (many diffs) |
| 8 | mouse | 18260 | Ocln | occludin | NM_001360537.1 | 85.8% | 89.6% | (many diffs) |
| 9 | mouse | 18260 | Ocln | occludin | NM_001360538.1 | 85.8% | 89.6% | (many diffs) |
| 10 | mouse | 18260 | Ocln | occludin | NM_008756.2 | 85.8% | 89.6% | (many diffs) |
| 11 | mouse | 18260 | Ocln | occludin | XM_006517566.3 | 85.8% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1632
- ORF length:
- 1566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atccaggcct cttgaaagtc cacctcctta caggcctgat gaattcaaac 121 cgaatcatta tgcaccaagc aatgacatat atggtggaga aatgcatgtt cgaccaatgc 181 tctctcagcc agcctactct ttttacccag aagatgaaat tcttcacttc tacaaatgga 241 cctctcctcc aggagtgatt cggatcctgt ctatgctcat tattgtgatg tgcattgcca 301 tctttgcctg tgtggcctcc acgcttgcct gggacagagg ctatggaact tcccttttag 361 gaggtagtgt aggctaccct tatggaggaa gtggctttgg tagctacgga agtggctatg 421 gctatggcta tggttatggc tatggctacg gaggctatac agacccaaga gcagcaaagg 481 gcttcatgtt ggccatggct gccttttgtt tcattgccgc gttggtgatc tttgttacca 541 gtgttataag atctgaaatg tccagaacaa gaagatacta cttaagtgtg ataatagtga 601 gtgctatcct gggcatcatg gtgtttattg ccacaattgt ctatataatg ggagtgaacc 661 caactgctca gtcttctgga tctctatatg gttcacaaat atatgccctc tgcaaccaat 721 tttatacacc tgcagctact ggactctacg tggatcagta ttcgtatcac tactgtgttg 781 tggatcccca ggaggccatt gccattgtac tggggttcat gattattgtg gcttttgctt 841 taataatttt ctttgctgtg aaaactcgaa gaaagatgga caggtatgac aagtccaata 901 ttttgtggga caaggaacac atttatgatg agcagccccc caatgtcgag gagtgggtta 961 aaaatgtgtc tgcaggcaca caggacgtgc cttcaccccc atctgactat gtggaaagag 1021 ttgacagtcc catggcatac tcttccaatg gcaaagtgaa tgacaagcgg ttttatccag 1081 agtcttccta taaatccacg ccggttcctg aagtggttca ggagcttcca ttaacttcgc 1141 ctgtggatga cttcaggcag cctcgttaca gcagcggtgg taactttgag acaccttcaa 1201 aaagagcacc TGCAAAGGGA AGAGCAGGAA GGTCAAAGAG AACAGAGCAA GATCACTATG 1261 AGACAGACTA CACAACTGGC GGCGAGTCCT GTGATGAGCT GGAGGAGGAC TGGATCAGGG 1321 AATATCCACC TATCACTTCA GATCAACAAA GACAACTGTA CAAGAGGAAT TTTGACACTG 1381 GCCTACAGGA ATACAAGAGC TTACAATCAG AACTTGATGA GATCAATAAA GAACTCTCCC 1441 GTTTGGATAA AGAATTGGAT GACTATAGAG AAGAAAGTGA AGAGTACATG GCTGCTGCTG 1501 ATGAATACAA TAGACTGAAG CAAGTGAAGG GATCTGCAGA TTACAAAAGT AAGAAGAATC 1561 ATTGCAAGCA GTTAAAGAGC AAATTGTCAC ACATCAAGAA GATGGTTGGA GACTATGATA 1621 GACAGAAAAC ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTTG AAAGTATTTC GATTTCTTGG 1741 CTTTATATAT CTTGTGGAAA GGACGAGCAT CCATCTACTC GCACACCTTC ACGCGTTAAG 1801 TCgacaatca acctctggat tacaaaattt gtgaaagatt