Construct: ORF TRCN0000469640

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014349.1_s317c1
Derived from:
ccsbBroadEn_06667
DNA Barcode:
GCATCCATCTACTCGCACACCTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
OCLN (100506658)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469640
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100506658 OCLN occludin NM_001205254.2 99.8% 99.8% 96G>A;698T>C
2 human 100506658 OCLN occludin NM_002538.4 99.8% 99.8% 96G>A;698T>C
3 human 100506658 OCLN occludin XM_017008913.2 89.5% 89.4% 96G>A;698T>C;728_729ins162
4 human 100506658 OCLN occludin XM_017008914.2 89.5% 89.4% 96G>A;698T>C;728_729ins162
5 human 100506658 OCLN occludin NM_001205255.1 51.9% 51.9% 0_1ins753
6 human 647859 OCLNP1 OCLN pseudogene 1 NR_026578.1 34.6% (many diffs)
7 mouse 18260 Ocln occludin NM_001360536.1 85.8% 89.6% (many diffs)
8 mouse 18260 Ocln occludin NM_001360537.1 85.8% 89.6% (many diffs)
9 mouse 18260 Ocln occludin NM_001360538.1 85.8% 89.6% (many diffs)
10 mouse 18260 Ocln occludin NM_008756.2 85.8% 89.6% (many diffs)
11 mouse 18260 Ocln occludin XM_006517566.3 85.8% 89.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1632
ORF length:
1566
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc atccaggcct cttgaaagtc cacctcctta caggcctgat gaattcaaac
121cgaatcatta tgcaccaagc aatgacatat atggtggaga aatgcatgtt cgaccaatgc
181tctctcagcc agcctactct ttttacccag aagatgaaat tcttcacttc tacaaatgga
241cctctcctcc aggagtgatt cggatcctgt ctatgctcat tattgtgatg tgcattgcca
301tctttgcctg tgtggcctcc acgcttgcct gggacagagg ctatggaact tcccttttag
361gaggtagtgt aggctaccct tatggaggaa gtggctttgg tagctacgga agtggctatg
421gctatggcta tggttatggc tatggctacg gaggctatac agacccaaga gcagcaaagg
481gcttcatgtt ggccatggct gccttttgtt tcattgccgc gttggtgatc tttgttacca
541gtgttataag atctgaaatg tccagaacaa gaagatacta cttaagtgtg ataatagtga
601gtgctatcct gggcatcatg gtgtttattg ccacaattgt ctatataatg ggagtgaacc
661caactgctca gtcttctgga tctctatatg gttcacaaat atatgccctc tgcaaccaat
721tttatacacc tgcagctact ggactctacg tggatcagta ttcgtatcac tactgtgttg
781tggatcccca ggaggccatt gccattgtac tggggttcat gattattgtg gcttttgctt
841taataatttt ctttgctgtg aaaactcgaa gaaagatgga caggtatgac aagtccaata
901ttttgtggga caaggaacac atttatgatg agcagccccc caatgtcgag gagtgggtta
961aaaatgtgtc tgcaggcaca caggacgtgc cttcaccccc atctgactat gtggaaagag
1021ttgacagtcc catggcatac tcttccaatg gcaaagtgaa tgacaagcgg ttttatccag
1081agtcttccta taaatccacg ccggttcctg aagtggttca ggagcttcca ttaacttcgc
1141ctgtggatga cttcaggcag cctcgttaca gcagcggtgg taactttgag acaccttcaa
1201aaagagcacc TGCAAAGGGA AGAGCAGGAA GGTCAAAGAG AACAGAGCAA GATCACTATG
1261AGACAGACTA CACAACTGGC GGCGAGTCCT GTGATGAGCT GGAGGAGGAC TGGATCAGGG
1321AATATCCACC TATCACTTCA GATCAACAAA GACAACTGTA CAAGAGGAAT TTTGACACTG
1381GCCTACAGGA ATACAAGAGC TTACAATCAG AACTTGATGA GATCAATAAA GAACTCTCCC
1441GTTTGGATAA AGAATTGGAT GACTATAGAG AAGAAAGTGA AGAGTACATG GCTGCTGCTG
1501ATGAATACAA TAGACTGAAG CAAGTGAAGG GATCTGCAGA TTACAAAAGT AAGAAGAATC
1561ATTGCAAGCA GTTAAAGAGC AAATTGTCAC ACATCAAGAA GATGGTTGGA GACTATGATA
1621GACAGAAAAC ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1681ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTTG AAAGTATTTC GATTTCTTGG
1741CTTTATATAT CTTGTGGAAA GGACGAGCAT CCATCTACTC GCACACCTTC ACGCGTTAAG
1801TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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