Construct: ORF TRCN0000469660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018425.1_s317c1
- Derived from:
- ccsbBroadEn_02176
- DNA Barcode:
- GCCGCCTCGGATATTAGTTAGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPH3AL (9501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469660
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_001190411.1 | 100% | 100% | |
| 2 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_006987.4 | 100% | 100% | |
| 3 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_001190412.2 | 90.7% | 90.7% | 349_350ins87 |
| 4 | human | 9501 | RPH3AL | rabphilin 3A like (without ... | NM_001190413.2 | 90.7% | 90.7% | 349_350ins87 |
| 5 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | NM_029548.4 | 80.3% | 78.1% | (many diffs) |
| 6 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_006533637.1 | 80.3% | 78.1% | (many diffs) |
| 7 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_006533638.3 | 80.3% | 78.1% | (many diffs) |
| 8 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_006533639.3 | 80.3% | 78.1% | (many diffs) |
| 9 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_006533640.3 | 80.3% | 78.1% | (many diffs) |
| 10 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_006533641.3 | 80.3% | 78.1% | (many diffs) |
| 11 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_006533642.3 | 80.3% | 78.1% | (many diffs) |
| 12 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_011249100.1 | 60.1% | 60.8% | (many diffs) |
| 13 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | NM_001291159.1 | 38.1% | 34.5% | (many diffs) |
| 14 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_011249101.1 | 38.1% | 34.5% | (many diffs) |
| 15 | mouse | 380714 | Rph3al | rabphilin 3A-like (without ... | XM_011249102.1 | 38.1% | 34.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1014
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgacacc atcttcggca gcgggaatga tcagtgggtt tgccccaatg 121 accggcagct tgcccttcga gccaagctgc agacgggctg gtccgtgcac acctaccaga 181 cggagaagca gaggaggaag cagcacctca gcccggcgga ggtggaggcc atcctgcagg 241 tcatccagag ggcagagcgg ctcgacgtcc tggagcagca gagaatcggg cggctggtgg 301 agcggctgga gaccatgagg cggaatgtga tggggaacgg cctgtcccag tgtctgctct 361 gcggggaggt gctgggcttc ctgggcagct cgtcggtgtt ctgcaaagac tgcaggaaga 421 aagtctgcac caaatgtggg atcgaggcct cccctggcca gaagcggccc ctgtggctgt 481 gtaagatctg cagtgagcaa agagaggtct ggaagaggtc gggggcctgg ttctacaaag 541 ggctccccaa gtatatcttg cccctgaaga cccctggccg agctgatgac ccccacttcc 601 gacctttgcc cacggaaccg gcagagcgag agcccagaag ctctgagacc agccgcatct 661 acacgtgggc ccgaggaaga gtggtttcca gtgacagtga cagtgactcg gatcttagcT 721 CCTCCAGCCT AGAGGACAGA CTCCCATCCA CTGGGGTCAG GGACCGGAAA GGCGACAAAC 781 CCTGGAAGGA GTCAGGTGGC AGCGTGGAGG CCCCCAGGAT GGGGTTCACC CACCCGCCGG 841 GCCACCTCTC TGGGTGCCAG AGCAGCCTGG CCAGTGGTGA GACGGGGACA GGCTCTGCTG 901 ACCCGCCAGG GGGACCCCGC CCCGGGCTGA CCCGAAGGGC CCCGGTAAAA GACACACCTG 961 GACGAGCCCC CGCTGCTGAC GCAGCTCCAG CAGGCCCCTC CAGCTGCCTG GGCTTGCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAG CCGCCTCGGA TATTAGTTAG CCGACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att