Construct: ORF TRCN0000469741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015475.1_s317c1
- Derived from:
- ccsbBroadEn_03986
- DNA Barcode:
- AGGAAACCCGCCGAGAAACTTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAG3L4 (64940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64940 | STAG3L4 | stromal antigen 3-like 4 (p... | NR_040586.1 | 20.9% | 1_258del;709_2143del | |
| 2 | human | 64940 | STAG3L4 | stromal antigen 3-like 4 (p... | NR_040585.1 | 20.2% | 1_258del;344_419del;785_2219del | |
| 3 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282716.1 | 11.6% | 9.1% | (many diffs) |
| 4 | human | 10734 | STAG3 | stromal antigen 3 | NM_012447.4 | 11.6% | 9.1% | (many diffs) |
| 5 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282717.1 | 11.6% | 9.1% | (many diffs) |
| 6 | human | 10734 | STAG3 | stromal antigen 3 | XM_011515742.1 | 11.6% | 9.1% | (many diffs) |
| 7 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011686.2 | 11.5% | 9% | (many diffs) |
| 8 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011683.2 | 11.5% | 9% | (many diffs) |
| 9 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011684.1 | 11.5% | 9% | (many diffs) |
| 10 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011685.1 | 11.5% | 9% | (many diffs) |
| 11 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282718.2 | 11.4% | 8.9% | (many diffs) |
| 12 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011687.1 | 11.3% | 8.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tggctggata gctacaagca agaccaggat gcaggatttc tggagcttgt 121 taactttttc atctgatctt gtggatgtaa aggactcggg ggactatccT CTGACAGCTC 181 CAGGTCTATC CTGGAAGAAG TTCCAGGGCA GCTTCTGTGA GTTTGTGGGG ACATTGGTCT 241 GTCGGTGCCA GTACATCCTC CTCCATGATG ACTTCCCTAT GGACAACCTC ATCTCCCTGC 301 TCACTGGCTT CTCAGACTCA CAAGTCTGCG CCTTCTGTCA CACTAGCACC CTGGCTGCTA 361 TGAAACTGAT GACCTCCCTG GTAAGAGTTG CCCTCCAACT GAGTCTGCAC GAAGATATCA 421 ATCAGCGTCA GTATGAGGCT GAAAGAAACA AGGGGCCAGG GCAGAGGGCA CCTGAGCGGC 481 TGGAGAGCCT GTTGGAGAAA CACAAAGAGC TGCATTACCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 AAGGAAACCC GCCGAGAAAC TTGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt