Transcript: Human NM_001282718.2

Homo sapiens stromal antigen 3 (STAG3), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
STAG3 (10734)
Length:
3989
CDS:
120..3623

Additional Resources:

NCBI RefSeq record:
NM_001282718.2
NBCI Gene record:
STAG3 (10734)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001282718.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230168 CCGCAGCCAGCTAGTAGATTT pLKO_005 1943 CDS 100% 13.200 18.480 N STAG3 n/a
2 TRCN0000137596 CGTGATTTCCTTAGGCCACTT pLKO.1 2400 CDS 100% 4.050 5.670 N STAG3 n/a
3 TRCN0000230169 GTGATCTACTTCTCATCTTTA pLKO_005 2356 CDS 100% 13.200 9.240 N STAG3 n/a
4 TRCN0000230170 TGATTCCCAGGAGGATCATTT pLKO_005 2510 CDS 100% 13.200 9.240 N STAG3 n/a
5 TRCN0000134797 CAGGAGGATCATTTACAGATA pLKO.1 2517 CDS 100% 4.950 3.465 N STAG3 n/a
6 TRCN0000138271 CCAGCCTTGACTCTTGTCTAT pLKO.1 2175 CDS 100% 4.950 3.465 N STAG3 n/a
7 TRCN0000135354 GCTGATGAAGACACTGACTTT pLKO.1 255 CDS 100% 4.950 3.465 N STAG3 n/a
8 TRCN0000135230 CAAGAGCATCAAGAGGAGATT pLKO.1 816 CDS 100% 4.950 2.970 N STAG3 n/a
9 TRCN0000121694 CCTCACCGACAGCTATTTAAA pLKO.1 962 CDS 100% 15.000 7.500 Y STAG3L3 n/a
10 TRCN0000062692 GCTATCTGCATTGAGGAAATT pLKO.1 909 CDS 100% 13.200 6.600 Y STAG3L1 n/a
11 TRCN0000139360 CAGCTTCTGCTGTCCTTCTTT pLKO.1 1380 CDS 100% 5.625 2.813 Y STAG3L3 n/a
12 TRCN0000139995 GAGATCCGTGCTATCTGCATT pLKO.1 900 CDS 100% 4.950 2.475 Y STAG3L3 n/a
13 TRCN0000062691 GCAAAGCTACAGCACGTCTTT pLKO.1 941 CDS 100% 4.950 2.475 Y STAG3L1 n/a
14 TRCN0000122408 GCAAAGCTACAGCACGTCTTT pLKO.1 941 CDS 100% 4.950 2.475 Y STAG3L3 n/a
15 TRCN0000138869 GCAAAGCTACAGCACGTCTTT pLKO.1 941 CDS 100% 4.950 2.475 Y STAG3 n/a
16 TRCN0000139256 CTGCATGATAAGCACCGAGAA pLKO.1 999 CDS 100% 4.050 2.025 Y STAG3L3 n/a
17 TRCN0000140844 CAGAGGACTTTCTTCCAGCTT pLKO.1 1365 CDS 100% 2.640 1.320 Y STAG3L3 n/a
18 TRCN0000167398 GAATTTCTGTACTGGAAACTT pLKO.1 1281 CDS 100% 5.625 2.813 Y STAG3L2 n/a
19 TRCN0000062690 CGCTGCTTACTTAGTAGACAA pLKO.1 1424 CDS 100% 4.950 2.475 Y STAG3L1 n/a
20 TRCN0000167941 CGCTGCTTACTTAGTAGACAA pLKO.1 1424 CDS 100% 4.950 2.475 Y STAG3L2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001282718.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07670 pDONR223 100% 95.1% 95.1% None (many diffs) n/a
2 ccsbBroad304_07670 pLX_304 0% 95.1% 95.1% V5 (many diffs) n/a
3 TRCN0000476860 CATCCTAGTGCATTCGAGCCGTGA pLX_317 11.7% 95.1% 95.1% V5 (many diffs) n/a
4 ccsbBroadEn_03986 pDONR223 100% 11.4% 8.9% None (many diffs) n/a
5 ccsbBroad304_03986 pLX_304 0% 11.4% 8.9% V5 (many diffs) n/a
6 TRCN0000469741 AGGAAACCCGCCGAGAAACTTGGA pLX_317 75.9% 11.4% 8.9% V5 (many diffs) n/a
7 ccsbBroadEn_10171 pDONR223 100% 10.2% 9.6% None (many diffs) n/a
8 ccsbBroad304_10171 pLX_304 0% 10.2% 9.6% V5 (many diffs) n/a
9 TRCN0000474301 GGCATGATAACGCGACACGTACAC pLX_317 60.6% 10.2% 9.6% V5 (many diffs) n/a
10 ccsbBroadEn_13704 pDONR223 100% 6.3% 5.8% None (many diffs) n/a
11 ccsbBroad304_13704 pLX_304 0% 6.3% 5.8% V5 (many diffs) n/a
12 ccsbBroadEn_12038 pDONR223 100% 6.2% 5.1% None (many diffs) n/a
13 ccsbBroad304_12038 pLX_304 0% 6.2% 5.1% V5 (many diffs) n/a
14 TRCN0000471778 CAAGTTTGACTAGCTTGAAGTGGC pLX_317 100% 6.2% 5.1% V5 (many diffs) n/a
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